Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato
M. Staniaszek, W. Szczechura, W. Marczewskihttps://doi.org/10.17221/70/2014-CJGPBCitation:Staniaszek M., Szczechura W., Marczewski W. (2014): Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato. Czech J. Genet. Plant Breed., 50: 285-287.
Fusarium oxysporum Schlecht. f.sp. radicis-lycopersici Jarvis & Schoemaker (FORL) is a saprophytic fungus, responsible for the fusarium crown and root rot disease in tomato (Solanum lycopersicum L.). This is one of the most destructive pathogens of this species. A new cleaved amplified polymorphic sequence (CAPS) marker C2-25 was developed for the detection of the dominant gene Frl, which confers tomato resistance to FORL. C2-25 was amplified from a conserved ortholog set II (COSII) sequence C2_At2g38025. The XapI-derived restriction product of 700 bp was informative for the identification of FORL resistant tomato genotypes and can be used as a diagnostic marker in tomato breeding programmes and hybrid seed production.Keywords:
CAPS marker; fusarium crown and root rot; marker assisted selection (MAS); Solanum lycopersicum L.