In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

Pranit Kumar Mukherjee; Raju Mondal; Sourav Dutta; Kanti Meena; Madhumita Roy; Asit Baran Mandal

doi:10.17221/79/2017-CJGPB

In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

Pranit Kumar Mukherjee, Raju Mondal, Sourav Dutta, Kanti Meena, Madhumita Roy, Asit Baran Mandal

https://doi.org/10.17221/79/2017-CJGPBCitation:Mukherjee P.K., Mondal R., Dutta S., Meena K., Roy M., Mandal A.B. (2018): In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation. Czech J. Genet. Plant Breed., 54: 183-189.

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An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO₃. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.

Keywords:

clonal fidelity; molecular markers; ramie; tissue culture; vegetative propagation

References:

Batra S.K., Bell C. (1975): On the Efﬁcient Utilization of Natural (Vegetable) Fibers. A Technology Adaptation Program Report. Cambridge, Massachusetts Institute of Technology.

Bradford Marion M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3

Chi Gek-Lan, Pua Eng-Chong (1989): Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (Chinese cabbage) in vitro. Plant Science, 64, 243-250 https://doi.org/10.1016/0168-9452(89)90030-7

Chi G.-L., Pua E.-C., Goh C.-J. (1991): Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro. PLANT PHYSIOLOGY, 96, 178-183 https://doi.org/10.1104/pp.96.1.178

Chraibi B.K.M., Latche A., Roustan J.P., Fallot J. (1991): Stimulation of shoot regeneration from cotyledons of Helianthus annuus by ethylene inhibitors, silver and cobalt. Plant Cell Reports, 10: 204–207.

Del Rosario A.G., Corcolon B.M. (1991): Clonal multiplication of ramie by tissue culture. Fibre Research Journal, 1: 28–35.

Deng G., Liu L., Wang H., Lao C., Wang B., Zhu C., Peng D. (2013): Establishment and optimization of two-dimensional electrophoresis (2-DE) technology for proteomic analysis of ramie. International Journal of Agriculture and Biology, 15: 570‒574.

Devendra N.K., Rajanna L., Sheetal C., Seetharam Y.N. (2008): In vitro clonal propagation of Trichosanthes cucumerina L.var. cucumerina. Plant Tissue Culture and Biotechnology, 18: 103–111.

Dumanois R.C., Boucher B.F., Cosson L., Paris M. (1986): Multiplication végétative in vitro du chanvre (Cannabis sativa L.). Application à la conservation des clones sélectionnés. Agronomie, 6: 487−495. (in Parsian)

Fontanelli D. (1998): Proprieta meccaniche e morfologia di fibre di Spartium junceum L. (Ginestra) in Italian. [Ph.D. Thesis.] Pisa, Department of Chemical Engineering, University of Pisa.

George E.F., Sherrington P.D. (1984): Plant propagation by tissue culture. In: Handbook and Dictionary of Commercial Laboratories. Basingstoke, Exegetics, Ltd.: 223–227.

Goda Koichi, Sreekala M.S., Gomes Alexandre, Kaji Takeshi, Ohgi Junji (2006): Improvement of plant based natural fibers for toughening green composites—Effect of load application during mercerization of ramie fibers. Composites Part A: Applied Science and Manufacturing, 37, 2213-2220 https://doi.org/10.1016/j.compositesa.2005.12.014

Hazarika B.N., Parthasarathy V.A., Nagaraju V., Bhowmik G. (2000): Sucrose induced biochemical changes in in vitro microshoots of Citrus species. Indian Journal of Horticulture, 57: 27–31.

LAEMMLI U. K. (1970): Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685 https://doi.org/10.1038/227680a0

Malek MA, Khanam D, Khatun M, Molla MH, Mannan MA (2010): In vitro culture of pointed gourd (Trichosanthes dioica Roxb.). Bangladesh Journal of Agricultural Research, 35, - https://doi.org/10.3329/bjar.v35i1.5874

Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Murray M.G., Thompson W.F. (1980): Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321-4326 https://doi.org/10.1093/nar/8.19.4321

Naik Soumendra K., Chand Pradeep K. (2003): Silver nitrate and aminoethoxyvinylglycine promotein vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). Journal of Plant Physiology, 160, 423-430 https://doi.org/10.1078/0176-1617-00949

Palmer C.E. (1992): Enhanced shoot regeneration from Brassica campestris by silver nitrate. Plant Cell Reports, 11, - https://doi.org/10.1007/BF00233088

Purnhauser L�szl�, Medgyesy P�ter, Czak� Mih�ly, Dix Philip J., M�rton L�szl� (1987): Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3. Plant Cell Reports, 6, 1-4 https://doi.org/10.1007/BF00269725

Saha Soumen, Kader Abdul, Sengupta Chandan, Ghosh Parthadeb (2012): In Vitro Propagation of Ocimum Gratissimum L. (Lamiaceae) and Its Evaluation of Genetic Fidelity Using RAPD Marker. American Journal of Plant Sciences, 03, 64-74 https://doi.org/10.4236/ajps.2012.31006

Singh S.K., Shymal M.M. (2001): Effect of media and physical factors on in vitro rooting in roses. Horticulture Journal, 14: 91–97.

Sut D., Talukdar P., Singh S. (2004): Micro-propagation of ramie (Boehmeria nivea (L.) Gaud) through shoot-tip and nodal segment culture. Indian Journal of Agriculture Research, 38: 131–134.

Wainwright H., Scrace J. (1989): Influence of in vitro preconditioning with carbohydrates during the rooting of microcuttings on in vivo establishment. Scientia Horticulturae, 38, 261-267 https://doi.org/10.1016/0304-4238(89)90073-3

Wang B., Peng D., Liu L., Sun Z., Zhang N., Gao S. (2007): An efﬁcient adventitious shoot regeneration system for ramie (Boehmeria nivea Gaud) using thidiazuron. Botanical Studies, 48: 173–180.

Yoshida S., Forna D.A., Cock J.A., Gomez K.A. (1972): Laboratory Manual for Physiological Studies on Rice. Manila, International Rice Research Institute.

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Czech Academy of Agricultural Sciences

In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

Pranit Kumar Mukherjee, Raju Mondal, Sourav Dutta, Kanti Meena, Madhumita Roy, Asit Baran Mandal

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