In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation
Pranit Kumar Mukherjee, Raju Mondal, Sourav Dutta, Kanti Meena, Madhumita Roy, Asit Baran Mandal
https://doi.org/10.17221/79/2017-CJGPBCitation:Mukherjee P.K., Mondal R., Dutta S., Meena K., Roy M., Mandal A.B. (2018): In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation. Czech J. Genet. Plant Breed., 54: 183-189.An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO3. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.
clonal fidelity; molecular markers; ramie; tissue culture; vegetative propagation
References:Impact factor (Web of Science):
2020: 0.865
Q4 – Agronomy
Q4 – Plant Sciences
5-Year Impact Factor: 1.017
SCImago Journal Rank (SCOPUS):
New Issue Alert
Join the journal on Facebook!
Ask for email notification.
Similarity Check
All the submitted manuscripts are checked by the CrossRef Similarity Check.
Referred to in
AGRICOLA
Agrindex of AGRIS/FAO database
CAB Abstracts, Czech Agricultural and Food Bibliography
CNKI
EBSCO – Academic Search Ultimate
DOAJ (Directory of Open Access Journals)
Science Citation Index Expanded®
SCOPUS
SI Web of KnowledgeSM
ProQuest
Web of Science®
Licence terms
All content is made freely available for non-commercial purposes, users are allowed to copy and redistribute the material, transform, and build upon the material as long as they cite the source.
Open Access Policy
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
Contact
Ing. Markéta Knížková
Executive Editor
phone: + 420 227 010 373
e-mail: cjgpb@cazv.cz
Address
Czech Journal of Genetics and Plant Breeding
Czech Academy of Agricultural Sciences
Slezská 7, 120 00 Praha 2, Czech Republic