Development of CAPS marker for identifi cation of rym4 and rym5 alleles conferring resistance to the Barley Yellow Mosaic Virus complex in barley
T. Sedláček, P. Mařík, J. Chrpováhttps://doi.org/10.17221/7/2010-CJGPBCitation:Sedláček T., Mařík P., Chrpová J. (2010): Development of CAPS marker for identifi cation of rym4 and rym5 alleles conferring resistance to the Barley Yellow Mosaic Virus complex in barley. Czech J. Genet. Plant Breed., 46: 159-163.
In barley, soil-borne viruses of the barley mosaic virus complex (BaYMV, BaMMV, BaYMV-2) are of high importance due to their increased spread, yield losses, and the fact that protection with chemicals is not possible. Concerning resistance breeding, simple, cheap, and robust selection methods are required. Markerassisted selection (MAS) has a great potential to meet this demand. A CAPS marker designed directly for SNPs causing mutations responsible for resistance of rym4/rym5 alleles of the Hv-eIF4E gene was developed in this study. Results showed perfect correspondence between the CAPS marker and the allele identified indirectly on the basis of field tests.Keywords:
barley; marker-assisted breeding; mosaic virus complex; resistance; rym allele identification