Reaction of Nicotiana species and cultivars of tobacco to Tobacco mosaic virus and detection of the N gene that confers hypersensitive resistance

https://doi.org/10.17221/81/2017-CJGPBCitation:Depta A., Kursa K., Doroszewska T., Laskowska D., Trojak-Goluch A. (2018): Reaction of Nicotiana species and cultivars of tobacco to Tobacco mosaic virus and detection of the N gene that confers hypersensitive resistance. Czech J. Genet. Plant Breed., 54: 143-146.
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Tobacco mosaic virus (TMV) brings increasing losses in the cultivation of tobacco. Sixty-two cultivars of tobacco and eleven species of Nicotiana were evaluated for resistance to TMV. Biological tests at two temperature ranges, DAS-ELISA and molecular markers were applied to assess the resistance to TMV. Most cultivars of tobacco showed susceptibility (S) to TMV, two were tolerant (T), while others revealed a hypersensitive response (HR). Hypersensitivity, determined by the N gene, occurred only at a temperature below 22°C. At a temperature above 28°C, all the cultivars showed mosaic discolorations or extensive necrosis. The reaction of the Nicotiana species was dependent on growth conditions. At 22°C, the reactions of sensitivity, tolerance and hypersensitivity to TMV were all observed, whereas above 28°C the species showed systemic necrotic symptoms. N. gossei was an exception because hypersensitivity occurred regardless of the thermal conditions. The resistance of this species was not conditioned by the N gene, which suggests that N. gossei could be an additional genetic resource for tobacco breeding.

 

References:
Clayton E.E., Smith H.H., Foster H.H. (1938): Mosaic resistance in Nicotiana tabacum L. Phytopathology, 28: 286–288.
 
Czubacka A., Doroszewska T. (2010): Combination of different sources of resistance to PVY in tobacco doubled haploids. In: Proc. CORESTA Joint Study Groups Meeting, Edinburgh, Sept, 12–16, 2010: AP-08.
 
Gera A., Tam Y., Teverovsky E., Loebenstein G. (1993): Enhanced tobacco mosaic virus production and suppressed synthesis of the inhibitor of virus replication in protoplasts and plants of local lesion responding cultivars exposed to 35 °C. Physiological and Molecular Plant Pathology, 43, 299-306  https://doi.org/10.1006/pmpp.1993.1059
 
Gwynn G.R. (1977): Evaluation of tobacco mosaic virus resistant germplasm. Tobacco Research, 3: 89–94.
 
Lewis R. S., Milla S. R., Levin J. S. (2005): Molecular and Genetic Characterization of L. Chromosome Segments in -Resistant Tobacco Accessions. Crop Science, 45, 2355-  https://doi.org/10.2135/cropsci2005.0121
 
Valleau W. D. (1952): Breeding tobacco for disease resistance. Economic Botany, 6, 69-102  https://doi.org/10.1007/BF02859199
 
Dijk P., Cuperus C. (1989): Reactions on Nicotiana species to potato viruses A, X and Y and tobacco mosaic virus in relation to their taxonomy and geographical origin. Netherlands Journal of Plant Pathology, 95, 343-356  https://doi.org/10.1007/BF01976621
 
White R. F., Sugars J. M. (1996): The Systemic Infection by Tobacco Mosaic Virus of Tobacco Plants Coutaining the N Gene at Temperatures Below 28°C. Journal of Phytopathology, 144, 139-142  https://doi.org/10.1111/j.1439-0434.1996.tb01503.x
 
Yuan Xinjie, Yan Chenghuan, Wu Zhujun, Ren Feihong, Zhang Hui, Baker Barbara, Chen Jiongjiong, Kuang Hanhui (2015): Frequent Gain and Loss of Resistance against Tobacco Mosaic Virus in Nicotiana Species. Molecular Plant, 8, 1813-1815  https://doi.org/10.1016/j.molp.2015.09.001
 
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