The effect of copper on plant regeneration in barley microspore culture K., Oleszczuk S., Zimny J. (2017): The effect of copper on plant regeneration in barley microspore culture. Czech J. Genet. Plant Breed., 53: 17-22.
download PDF
Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.
Caredda S., Doncoeur C., Devaux P., Sangwan R. S., Clément C. (2000): Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.). Sexual Plant Reproduction, 13, 95-104
Caredda S., Devaux P., Sangwan R.S., Proult I., Clemént C. (2004): Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare) cultivars. Plant Cell, Tissue and Organ Culture, 76: 35–43.
Castillo A.M., Valles M.P., Cistué L. (2000): Comparison of anther and isolated microspore cultures in barley. Effects of culture density and regeneration medium. Euphytica, 113: 1–8.
Castillo A. M., Nielsen N. H., Jensen A., Vallés M. P. (2014): Effects of n-butanol on barley microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC), 117, 411-418
Coronado María-José, Hensel Götz, Broeders Sylvia, Otto Ingrid, Kumlehn Jochen (2005): Immature pollen-derived doubled haploid formation in barley cv. Golden Promise as a tool for transgene recombination. Acta Physiologiae Plantarum, 27, 591-599
Daghma D. S., Kumlehn J., Hensel G., Rutten T., Melzer M. (): Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.). Journal of Experimental Botany, 63, 6017-6021
Devaux P., Kasha K.J. (2009): Overview of barley doubled haploid production. In: Touraev A., Forster B.P., Jain S.M. (eds): Advances in Haploid Production in Higher Plants. Heidelberg, Springer: 47–63.
Esteves Patricio, Clermont Isabelle, Marchand Suzanne, Belzile François (2014): Improving the efficiency of isolated microspore culture in six-row spring barley: II-exploring novel growth regulators to maximize embryogenesis and reduce albinism. Plant Cell Reports, 33, 871-879
Forster Brian P., Heberle-Bors Erwin, Kasha Ken J., Touraev Alisher (2007): The resurgence of haploids in higher plants. Trends in Plant Science, 12, 368-375
Germanà Maria Antonietta (2011): Anther culture for haploid and doubled haploid production. Plant Cell, Tissue and Organ Culture (PCTOC), 104, 283-300
González-Melendi Pablo, Ramírez Carmen, Testillano Pilar S., Kumlehn Jochen, Risueño María Carmen (2005): Three dimensional confocal and electron microscopy imaging define the dynamics and mechanisms of diploidisation at early stages of barley microspore-derived embryogenesis. Planta, 222, 47-57
Grauda D., Mikelsone A., Lsina N., Žagata K., Ornicāns R., Fokina O., Lapina L., Rashal I. (2014): Anther culture effectiveness in producing doubled haploids of cereals. Proceedings of the Latvian Academy of Sciences. Section B, 68: 142–147.
Hofinger B.J., Ankele E., Gully C., Heberle-Bors E., Pfosser M.F. (2000): The involvment of the plastid genome in albino plant regeneration from microspores on wheat. In: Bohanec B. (ed.): Biotechnological Approaches for Utilization of Gametic Cell, COST 824, Final Meeting, Bled, July 1–5, 2000: 215–228.
Jacquard Cédric, Nolin Frédérique, Hécart Carine, Grauda Dace, Rashal Isaak, Dhondt-Cordelier Sandrine, Sangwan Rajbir S., Devaux Pierre, Mazeyrat-Gourbeyre Florence, Clément Christophe (2009): Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars. Plant Cell Reports, 28, 1329-1339
Jähne Alwine, Lörz Horst (1995): Cereal microspore culture. Plant Science, 109, 1-12
Kasha K.J. (): Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores. Journal of Experimental Botany, 52, 1227-1238
Kumlehn Jochen, Serazetdinova Liliya, Hensel Goetz, Becker Dirk, Loerz Horst (2006): Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens. Plant Biotechnology Journal, 4, 251-261
Lantos Csaba, Bóna Lajos, Boda Krisztina, Pauk János (2014): Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters. Euphytica, 197, 27-37
Larsen E. T., Tuvesson I. K. D., Andersen S. B. (1991): Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture. Theoretical and Applied Genetics, 82, 417-420
Li Hongchao, Devaux Pierre (2003): High frequency regeneration of barley doubled haploid plants from isolated microspore culture. Plant Science, 164, 379-386
Li Hongchao, Devaux Pierre (2005): Isolated microspore culture overperforms anther culture for green plant regeneration in barley (Hordeum vulgare L.). Acta Physiologiae Plantarum, 27, 611-619
Makowska Katarzyna, Oleszczuk Sylwia (2014): Albinism in barley androgenesis. Plant Cell Reports, 33, 385-392
Makowska Katarzyna, Oleszczuk Sylwia, Zimny Aleksandra, Czaplicki Andrzej, Zimny Janusz, Hartl L. (2015): Androgenic capability among genotypes of winter and spring barley. Plant Breeding, 134, 668-674
Maksymiec W. (1998): Effect of copper on cellular processes in higher plants. Photosynthetica, 34: 321–342.
Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (2003): Doubled Haploid Production in Crop Plants: A Manual. Dordrecht, Kluwer : 309–335.
Muñoz-Amatriaín M., Castillo A. M., Chen X. W., Cistué L., Vallés M. P. (2008): Identification and validation of QTLs for green plant percentage in barley (Hordeum vulgare L.) anther culture. Molecular Breeding, 22, 119-129
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497
Nuutila A.M, Hämäläinen J, Mannonen L (2000): Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.). Plant Science, 151, 85-92
Oleszczuk S., Sowa S., Zimny J. (2006): Androgenic response to preculture stress in microspore cultures of barley. Protoplasma, 228, 95-100
Ritala A., Mannonen L., Oksman-Caldentey K.-M. (2001): Factors affecting the regeneration capacity of isolated barley microspores (Hordeum vulgare L.). Plant Cell Reports, 20, 403-407
Wojnarowiez G., Jacquard C., Devaux P., Sangwan R.S., Clément C. (2002): Influence of copper sulfate on anther culture in barley (Hordeum vulgare L.). Plant Science, 162, 843-847
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti