Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding N., Xu B.-., Bi Y.-., Lou Q.-., Chen J.-., Qian C.-., Zhang Y.-., Yi H.-. (2017): Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding. Czech J. Genet. Plant Breed., 53: 23-29.
download PDF

Gummy stem blight (GSB), caused by Didymella bryoniae (Auersw.) Rehm., is a severe disease affecting Cucurbitaceae crops including melons. The resistance of current melon varieties that carry a single Gsb resistance gene is insufficient to protect against the abundant variation of the D. bryoniae isolates. Pyramiding multiple Gsb resistance genes into melon cultivars is an effective way to develop a broad resistance spectrum and to increase the duration of GSB resistance. In this study, two resistance genes (Gsb-4 and Gsb-6) from two resistant accessions, PI482398 and PI420145, were pyramided into one variety using marker-assisted selection (MAS). The donor parent 4598 that contained Gsb-4 and Gsb-6 was hybridized with muskmelon Baipicui to produce BC1F1 and BC2F1. Phenotyping and MAS enabled identification and pyramiding of two Gsb genes in individuals of F1, BC1F1 and BC2F1. Field cultivation showed that individuals carrying both Gsb-4 and Gsb-6 had improved resistance to GSB and improved fruit quality. The results indicated that MAS-based pyramiding is an effective strategy for breeding melon cultivars with increased resistance to GSB.

Amand P.C., Wehner T.C. (1991): Crop loss to 14 diseases of cucumber in North Carolina from 1983 to 1988. Cucurbit Genetics Cooperative Report, 14: 15–17.
Ashutosh , Sharma P. C., Prakash Shyam, Bhat S. R. (2006): Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia arvensis) Brassica juncea and conversion to SCAR marker. Theoretical and Applied Genetics, 114, 385-392
Frantz J. D., Jahn M. M. (2004): Five independent loci each control monogenic resistance to gummy stem blight in melon ( Cucumis melo L.). TAG Theoretical and Applied Genetics, 108, 1033-1038
Gusmini G., Ellington T.L., Wehner T.C. (2003): Mass production of gummy stem blight spores for resistance screening. Cucurbit Genetics Cooperative Report, 26: 26–30.
Gusmini Gabriele, Song Ronghao, Wehner Todd C. (2005): New Sources of Resistance to Gummy Stem Blight in Watermelon. Crop Science, 45, 582-
Hanson Peter, Lu Shu-Fen, Wang Jaw-Fen, Chen Wallace, Kenyon Lawrence, Tan Chee-Wee, Tee Kwee Lian, Wang Ya-Ying, Hsu Yun-Che, Schafleitner Roland, Ledesma Dolores, Yang Ray-Yu (2016): Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Scientia Horticulturae, 201, 346-354
Jena K. K., Mackill D. J. (2008): Molecular Markers and Their Use in Marker-Assisted Selection in Rice. Crop Science, 48, 1266-
Kang Byoung-Cheorl, Yeam Inhwa, Jahn Molly M. (2005): Genetics of Plant Virus Resistance. Annual Review of Phytopathology, 43, 581-621
Keinath A. (1996): Morphological, Pathological, and Genetic Differentiation of Didymella bryoniae and Phoma spp. Isolated from Cucurbits. Phytopathology, 85, 364-
Lebeda A. (1985): Dangerous and lesser known pathogen of cucumbers. Záhradníctvo 10, 122–124. (in Czech)
McDonald B.A., Linde C. (2002): The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica, 124: 163–180.
Mienie C.M.S., Fourie H., Smit M.A., Van Staden J., Botha F.C. (2002): Identification of AFLP markers in soybean linked to resistance to Meloidogyne javanica and conversion to Sequence Characterized Amplified Regions (SCARs). Plant Growth Regulation, 37: 157–166.
Nowicki Marcin, Foolad Majid R., Nowakowska Marzena, Kozik Elzbieta U. (2012): Potato and Tomato Late Blight Caused by Phytophthora infestans : An Overview of Pathology and Resistance Breeding. Plant Disease, 96, 4-17
Rogers S.O., Bendich A.J. (1994): Extraction of total cellular DNA from plants, algae and fungi. In: Plant Molecular Biology Manual. Dordrecht, Kluwer Academic Publishers: 1–8.
Sitterly W.R., Keinath A.P. (1996): Gummy stem blight. In: Zitter T.A., Hopkins D.L., Thomas C.E. (eds): Compendium of Cucurbit Diseases. St. Paul, American Phytopathological Society: 27–28.
Sowell Grover (1981): Additional Sources of Resistance to Gummy Stem Blight of Muskmelon. Plant Disease, 65, 253-
Suh Jung-Pil, Jeung Ji-Ung, Noh Tae-Hwan, Cho Young-Chan, Park So-Hyun, Park Hyun-Su, Shin Mun-Sik, Kim Chung-Kon, Jena Kshirod K (2013): Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice. Rice, 6, 5-
Tsutsumi Cláudio Yuji, Silva Norberto da (2004): Screening of melon populations for resistance to Didymella bryoniae in greenhouse and plastic tunnel conditions. Brazilian Archives of Biology and Technology, 47, -
Vidavski F., Czosnek H., Gazit S., Levy D., Lapidot M. (2008): Pyramiding of genes conferring resistance to Tomato yellow leaf curl virus from different wild tomato species. Plant Breeding, 127, 625-631
Vos Pieter, Hogers Rene, Bleeker Marjo, Reijans Martin, Lee Theo van de, Hornes Miranda, Friters Adrie, Pot Jerina, Paleman Johan, Kuiper Martin, Zabeau Marc (1995): AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23, 4407-4414
Wang H.Y., Qian C.T., Lou L.N., Lou Q.F., Zhang Y.B., Yi H.P., Wu M.Z., Chen J.F. (2012): A SSR marker linked to Gsb-4 loci resistance to gummy stem blight in melon. Acta Horticulturae Sinica, 39: 574–580. (in Chinese)
Wolukau J.N. (2007): Genetic, phylogenetic and molecular studies of melon (Cucumis melo L.) resistance to gummy stem blight (Didymella bryoniae). [PhD. Thesis.] Nanjing, Nanjing Agriculture University.
Ye G.Y., Smith K.F. (2010): Marker-assisted gene pyramiding for cultivar development. Plant Breeding Reviews, 33: 219–256.
Zhang L.J, Fan J.J. (2007): Plant Physiology Experiments Tutorial. Beijing, China Agricultural University Press. (in Chinese)
Zhang Y.P., Kyle M., Anagnostou K., Zitter T.A. (1997): Screening melon (Cucumis melo L.) for resistance to gummy stem blight in the greenhouse and field. HortScience, 32: 117–121.
Zuniga T. L., Jantz J. P., Zitter T. A., Jahn M. K. (1999): Monogenic Dominant Resistance to Gummy Stem Blight in Two Melon ( Cucumis melo ) Accessions. Plant Disease, 83, 1105-1107
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti