Uni- and multivariate approaches to evaluating the susceptibility of wheat hybrids to Fusarium head blight
M. Surma, T. Adamski, H. Wiśniewska, Z. Kaczmarek, I. Mejza, S. Mejza, A. Kuczyńska, K. Krystkowiak, K. Mikołajczak, P. Ogrodowiczhttps://doi.org/10.17221/86/2016-CJGPBCitation:Surma M., Adamski T., Wiśniewska H., Kaczmarek Z., Mejza I., Mejza S., Kuczyńska A., Krystkowiak K., Mikołajczak K., Ogrodowicz P. (2016): Uni- and multivariate approaches to evaluating the susceptibility of wheat hybrids to Fusarium head blight. Czech J. Genet. Plant Breed., 52: 132-138.
Fusarium head blight (FHB) is a disease of small grain cereals caused by Fusarium species. The pathogens affect spikes and kernels, resulting in reductions of yield and its quality. The present study was conducted to evaluate variation in the FHB resistance of wheat F2 hybrids derived from 16 crosses between winter wheat cultivars of various origin and with different susceptibility to FHB. Plants were inoculated with a conidial suspension consisting of a mixture of F. culmorum, F. graminearum and F. avenaceum isolates. After harvest 1000-kernel weight, number of kernels per spike and kernel weight per spike were evaluated in inoculated and control plants. Disease symptoms were observed on kernels of infected plants and the percentage of Fusarium-damaged kernels (FDK) was calculated. The data were statistically evaluated using uni- and multivariate analyses. A significant influence of genotype and treatment on all observed characteristics was detected. Contrasts between control and inoculated plants showed that inoculation lowered the mean values of all the yield-related traits significantly (in the statistical sense). Results of uni- and multivariate analyses enabled us to find three cross combinations which exhibited a low FDK percentage and simultaneously a relatively low reduction of 1000-kernel weight after inoculation. They may be promising for breeding wheat with improved resistance to FHB.Keywords:
ANOVA; kernel damage; MANOVA; resistance to Fusarium head blight; winter wheatReferences:
Bottalico A., Perrone G. (2002): Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108: 998–1003. https://doi.org/10.1023/A:1020635214971Buerstmayr H., Ban T., Anderson J. A. (2009): QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breeding, 128, 1-26 https://doi.org/10.1111/j.1439-0523.2008.01550.xCaliński T. (1982): Dendrites. In: Kotz S., Johnson N.I. (eds): Encyclopedia of Statistical Sciences. Vol. 2, New York, John Wiley & Sons: 302–305.Caliński T., Kaczmarek Z. (1973): Statistical methods in the analysis of multivariate experiments. In: 3rd Methodological Colloquium in Agro-Biometrics, PAS, Warsaw, Sept 10–15, 1973: 258–320. (in Polish)Chełkowski J., Wiśniewska H., Adamski T., Goliński P., Kaczmarek Z., Kostecki M., Perkowski J., Surma M. (2000): Effects of Fusarium culmorum head blight on mycotoxin accumulation and yield traits in barley doubled haploids. Journal of Phytopathology, 148, 541-545 https://doi.org/10.1046/j.1439-0434.2000.00557.xCowger Christina, Patton-Özkurt Jennifer, Brown-Guedira Gina, Perugini Leandro (2009): Post-Anthesis Moisture Increased Fusarium Head Blight and Deoxynivalenol Levels in North Carolina Winter Wheat. Phytopathology, 99, 320-327 https://doi.org/10.1094/PHYTO-99-4-0320Hanna Anita Dobek, Kaczmarek Zygmunt, Kielczewska H. (1989): Genetic Analysis of Line x Tester Progenies Compared in Orthogonally Supplemented Efficiency Balanced Incomplete Block Designs. Biometrical Journal, 31, 753-757 https://doi.org/10.1002/bimj.4710310613Gosman N., Bayles R., Jennings P., Kirby J., Nicholson P. (2007): Evaluation and characterization of resistance to fusarium head blight caused by Fusarium culmorum in UK winter wheat cultivars. Plant Pathology, 56, 264-276 https://doi.org/10.1111/j.1365-3059.2006.01508.xGriffing B (1956): Concept of General and Specific Combining Ability in Relation to Diallel Crossing Systems. Australian Journal of Biological Sciences, 9, 463- https://doi.org/10.1071/BI9560463Kosová K., Chrpová J., Šíp V. (2009): Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding. Czech Journal of Genetics and Plant Breeding, 45: 87–105.Ma H.-X., Bai G.-H., Zhang X., Lu W.-Z. (2006): Main Effects, Epistasis, and Environmental Interactions of Quantitative Trait Loci for Fusarium Head Blight Resistance in a Recombinant Inbred Population. Phytopathology, 96, 534-541 https://doi.org/10.1094/PHYTO-96-0534Mardi M., Buerstmayr H., Ghareyazie B., Lemmens M., Mohammadi S. A., Nolz R., Ruckenbauer P. (2005): QTL analysis of resistance to Fusarium head blight in wheat using a 'Wangshuibai'-derived population. Plant Breeding, 124, 329-333 https://doi.org/10.1111/j.1439-0523.2005.01103.xMarin S., Ramos A.J., Cano-Sancho G., Sanchis V. (2013): Mycotoxins: Occurrence, toxicology, and exposure assessment. Food and Chemical Toxicology, 60, 218-237 https://doi.org/10.1016/j.fct.2013.07.047Mesterhazy A. (1995): Types and components of resistance to Fusarium head blight of wheat. Plant Breeding, 114, 377-386 https://doi.org/10.1111/j.1439-0523.1995.tb00816.xMesterházy A. (2002): Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. European Journal of Plant Pathology, 108: 675–684. https://doi.org/10.1023/A:1020631114063Mesterhazy A., Bartok T., Mirocha C. G., Komoroczy R. (1999): Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding, 118, 97-110 https://doi.org/10.1046/j.1439-0523.1999.118002097.xMesterházy Akos, Lehoczki-Krsjak Szabolcs, Varga Mónika, Szabó-Hevér Ágnes, Tóth Beata, Lemmens Marc (2015): Breeding for FHB Resistance via Fusarium Damaged Kernels and Deoxynivalenol Accumulation as Well as Inoculation Methods in Winter Wheat. Agricultural Sciences, 06, 970-1002 https://doi.org/10.4236/as.2015.69094Miedaner T. (1997): Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding, 116, 201-220 https://doi.org/10.1111/j.1439-0523.1997.tb00985.xMiedaner , Reinbrecht , Lauber , Schollenberger , Geiger (2001): Effects of genotype and genotype-environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breeding, 120, 97-105 https://doi.org/10.1046/j.1439-0523.2001.00580.xMorrison D.F. (1976): Multivariate Statistical Methods. 2nd Ed., Tokyo, McGraw-Hill Kogakusha, Ltd.Snijders C.H.A. (2004): Resistance in wheat to Fusarium infection and trichothecene formation. Toxicology Letters, 153, 37-46 https://doi.org/10.1016/j.toxlet.2004.04.044Tamburic-Ilincic L., Falk D., Schaafsma A. (2011): Fusarium ratings in Ontario Winter Wheat Performance Trial (OWWPT) using index that combines Fusarium head blight symptoms and deoxynivalenol levels. Czech Journal of Genetics and Plant Breeding, 47 (Special Issue): 115–122.Varshney R. K., Prasad M., Roy J. K., Kumar N., Not Available Not Available, Dhaliwal H. S., Balyan H.S., Gupta P.K. (2000): Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTL for grain weight in bread wheat. TAG Theoretical and Applied Genetics, 100, 1290-1294 https://doi.org/10.1007/s001220051437Warzecha T., Adamski T., Kaczmarek Z., Surma M., Chełkowski J., Wiśniewska H., Krystkowiak K., Kuczyńska A. (2011): Genotype-by-environment interaction of barley DH lines infected with Fusarium culmorum (W.G.Sm.) Sacc.. Field Crops Research, 120, 21-30 https://doi.org/10.1016/j.fcr.2010.08.009Wiśniewska Halina, Surma Maria, Krystkowiak Karolina, Adamski Tadeusz, Kuczyńska Anetta, Ogrodowicz Piotr, Mikołajczak Krzysztof, Belter Jolanta, Majka Maciej, Kaczmarek Zygmunt, Krajewski Paweł, Sawikowska Aneta, Lenc Leszek, Baturo-Cieśniewska Anna, Łukanowski Aleksander, Góral Tomasz, Sadowski Czesław (2016): Simultaneous selection for yield-related traits and susceptibility to Fusarium head blight in spring wheat RIL population. Breeding Science, 66, 281-292 https://doi.org/10.1270/jsbbs.66.281