Wheat leaf rust (Puccinia triticina Eriks.) virulence frequency and detection of resistance genes in wheat cultivars registered in the Czech Republic in 2016–2018

https://doi.org/10.17221/86/2019-CJGPBCitation:Hanzalová A., Dumalasová V., Zelba O. (2020): Wheat leaf rust (Puccinia triticina Eriks.) virulence frequency and detection of resistance genes in wheat cultivars registered in the Czech Republic in 2016–2018. Czech J. Genet. Plant Breed., 56: 87-92.
download PDF

In 2016–2018 virulence of the Czech wheat leaf rust population was studied on Thatcher near-isogenic lines, carrying different Lr genes, and 130 leaf rust isolates. Virulence to Lr9 was found only sporadically. Virulence frequency to Lr2a, Lr2b, Lr2c and Lr28 was lower than in previous years. All tested isolates were avirulent to Lr19. Lr24 conditioned resistance to majority of isolates. Nineteen recently registered Czech cultivars were tested with six isolates of the pathogen and Lr genes were postulated. Presence of genes Lr1, Lr10, Lr19, Lr24, Lr26, Lr28, Lr34 and Lr37 was tested by molecular markers. Lr37 prevailed, followed by Lr genes 10, 24, 28, 1 and 26; genes Lr19 and Lr34 were not determined.

Cherukuri D.P., Gupta S.K., Charpe A., Koul S., Prabhu K.V., Singh R.B., Haq Q.M.R. (2005): Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica, 143: 19–26. https://doi.org/10.1007/s10681-005-1680-6
de Froidmont D. (1998): A co-dominant marker for the 1BL/1RS wheat-rye translocation via multiplex PCR. Journal of Cereal Science, 27: 229–232.  https://doi.org/10.1006/jcrs.1998.0194
Goyeau H., Lannou C. (2011): Specific resistance to leaf rust expressed at the seedling stage in cultivars grown in France from 1983 to 2007. Euphytica, 178: 45–62. https://doi.org/10.1007/s10681-010-0261-5
Gultyaeva E.I., Kanyuka I.A., Alpateva N.V., Baranova O.A., Dmitriev A.P., Pavlyushin V.A. (2009): Molecular approaches in identifying leaf rust resistance genes in Russian wheat varieties. Plant Industry, 35: 316–319. https://doi.org/10.3103/S1068367409050085
Gupta S.K., Charpe A., Prabhu K.V., Haque Q.M.R. (2006a): Identification and validation of molecular marker linked to the leaf rust resistance gene Lr19 in wheat. Theoretical and Applied Genetics, 113: 1027–1036. https://doi.org/10.1007/s00122-006-0362-7
Gupta S.K., Charpe A., Koul S., Haque Q.M.R., Prabhu K.V. (2006b): Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica, 150: 233–240. https://doi.org/10.1007/s10681-006-9113-8
Hanzalová A., Bartoš P., Sumíková T. (2017): Physiologic specialization of wheat leaf rust and resistance of registered cultivars in the Czech Republic in 2012–2015. Czech Journal of Genetics and Plant Breeding, 53: 122–126. https://doi.org/10.17221/121/2016-CJGPB
Helguera M., Khan I.A., Kolmer J., Lijavetzky D., Zhong-Gi L., Dubcovsky J. (2003): PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Science, 43: 1839–1847. https://doi.org/10.2135/cropsci2003.1839
Huerta-Espino J., Singh R.P., Germán S., McCallum B.D., Park R.F., Chen W.Q., Bhardway S.C., Goyeau H. (2011): Global status of wheat leaf rust caused by Puccinia triti-cina. Euphytica, 179: 143–160. https://doi.org/10.1007/s10681-011-0361-x
Kolmer J.A., Hanzalova A., Goyeau H., Bayles R., Morgounov A. (2013): Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Europe. Plant Pathology, 62: 21–31. https://doi.org/10.1111/j.1365-3059.2012.02626.x
Lagudah E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. (2006): Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics, 114: 21–30. https://doi.org/10.1007/s00122-006-0406-z
McDonald B.A., Linde C. (2002): Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review of Phytopathology, 40: 349–379. https://doi.org/10.1146/annurev.phyto.40.120501.101443
McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. (2016): Catalogue of gene symbols for wheat: 2015–2016 Supplement. Available at https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2015.pdf
McIntosh R.A., Wellings C.R., Park R.F. (1995): Wheat Rusts: An Atlas of Resistance Genes. East Melbourne, CSIRO.
Mesterházy A., Bartoš P., Goyeau H., Niks R., Csösz M., Andersen O., Casulli F., Ittu M., Jones E., Manisterski J., Manninger K., Pasquini M., Rubiales D., Schachermayr G., Strzembicka A., Szunics L., Todorova M., Unger O., Vančo B., Vida G., Walther U. (2000): European virulence survey for leaf rust in wheat. Agronomie, 20: 793–804. https://doi.org/10.1051/agro:2000104
Qiu J.-W., Schürch A.Ch., Yahiaoui N., Dong L.-L., Fan H.-J., Zhang Z.-J., Keller B., Ling H.-Q. (2007): Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theoretical and Applied Genetics, 115: 159–168. https://doi.org/10.1007/s00122-007-0551-z
Roefls A.P., Singh R.P., Saari E.E. (1992): Rust Disease of Wheat: Concepts and Methods of Management. Mexico D.F., CIMMYT.
Serfling A., Krämer I., Volker L., Schliephake E., Ordon F. (2011): Diagnostic value of molecular markers for Lr genes and characterization of leaf rust resistance of German winter wheat cultivars with regard to the stability of vertical resistance. European Journal of Plant Pathology, 130: 559–575. https://doi.org/10.1007/s10658-011-9778-2
Stakman E.C., Stewart P.M., Loegering W.O. (1962): Identification of Physiologic Races of Puccinia graminis var. tritici. Agricultural Research Service E617, Washington D.C., USDA.
download PDF

© 2020 Czech Academy of Agricultural Sciences