An RNA-Seq analysis of the peach transcriptome with a focus on genes associated with skin colour
Red pigmentation of the skin is an important consumer trait in peach (Prunus persica). The pigment consists largely of anthocyanin. Here, a transcriptomic contrast, based on RNA-Seq technology, was drawn between a white-skinned (Feitao) and a red-skinned (Qiuxue) peach cultivar. The analysis identified 2407 genes as differentially transcribed in the fruit skin of the two cultivars. Among these were a number of genes known to contribute to anthocyanin synthesis. A quantitative real-time PCR assay was used to validate the RNA-Seq-based estimates of transcript abundance for 14 differentially transcribed genes. Anthocyanin synthesis was observed in the skin of Qiuxue fruit during the late ripening stage, matching the high transcript abundance of the gene encoding UDP glucose: flavonoid 3-O-glucosyltransferase, the final step in the synthesis of anthocyanin.
anthocyanin; pericarp colour; Prunus persica; sequencing
Dai H., Han G., Yan Y., Zhang F., Liu Z. (2013): Transcript assembly and quantification by RNA-Seq reveals differentially expressed genes between soft-endocarp and hard-endocarp hawthorns. PLoS ONE, 8: e72910. https://doi.org/10.1371/journal.pone.0072910
Kobayashi S., Ishimaru M., Hiraoka K., Honda C. (2002): Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis. Planta, 215: 924–933. https://doi.org/10.1007/s00425-002-0830-5
Liu G., Li W., Zheng P., Xu T., Chen L. (2012): Transcriptomic analysis of ‘Suli’ pear (Pyrus pyrifolia white pear group) buds during the dormancy by RNA-Seq. BMC Genomics, 13: 700. https://doi.org/10.1186/1471-2164-13-700
Rahim M.A., Busatto N., Trainotti L. (2014): Regulation of anthocyanin biosynthesis in peach fruits. Planta, 240: 913–929. https://doi.org/10.1007/s00425-014-2078-2
Tsuda T., Yamaguchi M., Honda C., Moriguchi T. (2004): Expression of anthocyanin biosynthesis genes in the skin of peach and nectarine fruit. Journal of the American Society for Horticultural Science, 129: 857–862. https://doi.org/10.21273/JASHS.129.6.0857
Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F., Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F. (2013): The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nature Genetics, 45: 487–494. https://doi.org/10.1038/ng.2586
Welch C.R., Wu Q., Simon J.E. (2008): Recent advances in anthocyanin analysis and characterization. Current Analytical Chemistry, 4: 75. https://doi.org/10.2174/157341108784587795
Zhang Q., Chen W., Sun L., Zhao F., Huang B., Yang W., Tao Y., Wang J. (2012): The genome of Prunus mume. Nature Communications, 3: 1318. https://doi.org/10.1038/ncomms2290
Zhou Y., Guo D., Li J., Cheng J., Zhou H., Gu C., Gardiner S., Han Y. (2013): Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea). Tree Genetics & Genomes, 9: 265–278.