SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon F., Garramone R., Aversano R., Carputo D.: (2017): SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon. Czech J. Genet. Plant Breed., 53: 168-171.
download PDF
In this study, 12 microsatellite markers (SSR) were evaluated for their applicability to protect from frauds and misuse the Italian PGI product “Common bean from Lamon”. SSR analysis generated polymorphic alleles, with an average of 4 alleles per locus and all in the range of molecular weight between 181 and 284 bp. Twenty-nine variety-specific fragments were identified, which might be reasonably adopted for characterization and traceability purposes. Cluster analysis well outlined the relationships between the genotypes studied. Overall, our study underlines the use and usefulness of molecular markers to protect both farmers and consumers from frauds.
Adamo Paola, Zampella Mariavittoria, Quétel Christophe R., Aversano Riccardo, Dal Piaz Fabrizio, De Tommasi Nunziatina, Frusciante Luigi, Iorizzo Massimo, Lepore Laura, Carputo Domenico (2012): Biological and geochemical markers of the geographical origin and genetic identity of potatoes. Journal of Geochemical Exploration, 121, 62-68
Angioi S. A., Rau D., Attene G., Nanni L., Bellucci E., Logozzo G., Negri V., Spagnoletti Zeuli P. L., Papa R. (2010): Beans in Europe: origin and structure of the European landraces of Phaseolus vulgaris L.. Theoretical and Applied Genetics, 121, 829-843
Carputo D., Alioto D., Aversano R., Garramone R., Miraglia V., Villano C., Frusciante L. (2013): Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers. Plant Genetic Resources, 11, 131-139
Guerra-Sanz J. M. (2004): New SSR markers of Phaseolus vulgaris from sequence databases. Plant Breeding, 123, 87-89
Ibarra-Perez Francisco J., Ehdaie Bahman, Waines J. Giles (1997): Estimation of Outcrossing Rate in Common Bean. Crop Science, 37, 60-
Kwak Myounghai, Gepts Paul (2009): Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae). Theoretical and Applied Genetics, 118, 979-992
Mercati Francesco, Leone Maria, Lupini Antonio, Sorgonà Agostino, Bacchi Monica, Abenavoli Maria Rosa, Sunseri Francesco (2013): Genetic diversity and population structure of a common bean (Phaseolus vulgaris L.) collection from Calabria (Italy). Genetic Resources and Crop Evolution, 60, 839-852
Piergiovanni Angela R., Lioi Lucia (2010): Italian Common Bean Landraces: History, Genetic Diversity and Seed Quality. Diversity, 2, 837-862
Varshney Rajeev K., Graner Andreas, Sorrells Mark E. (2005): Genic microsatellite markers in plants: features and applications. Trends in Biotechnology, 23, 48-55
Wang Aihua, Ding Yi, Hu Zhenhua, Lin Chufa, Wang Shuzhen, Wang Bingcai, Zhang Hongyuan, Zhou Guolin (2012): Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. International Journal of Molecular Sciences, 13, 11188-11193
download PDF

© 2018 Czech Academy of Agricultural Sciences