Grain hardness in triticale: a physical and molecular evaluationíková V., Vyhnánek T., Hanáček P., Martinek P. (2020): Grain hardness in triticale: a physical and molecular evaluation. Czech J. Genet. Plant Breed., 56: 102-110.
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Grain hardness is an important quality trait of cereals. In the present work, the particle size index (PSI) was used to assess the variability in grain hardness of thirteen triticale genotypes, three wheat genotypes and one rye genotype grown in two subsequent seasons. The PSI values of the triticale samples ranged between 7.7 to 19.2%, representing a medium hard to a very hard grain. Moreover, the sequencing of genes in the Hardness (Ha) locus revealed a limited genetic variability in the grain hardness genes, namely secaloindoline-a (Sina) and secaloindoline-b (Sinb). Two possible new allelic variants were identified, one for each of the secaloindoline genes. The existing variability in the grain hardness as well as polymorphisms in its candidate genes are a good starting point for efforts in breeding new varieties of triticale with improved grain hardness.

Barneveld R.J. van, Cooper K.V. (2002): Nutritional quality of triticale for pigs and poultry. In: Arseniuk E. (ed.): Proc. 5th Int Triticale Symposium, Radzików, June 30–July 5, 2002: 277–282.
Bassu S., Asseng S., Richards R. (2011): Yield benefits of triticale traits for wheat under current and future climates. Field Crops Research, 124: 14–24.
Beecher B., Bowman J., Martin J., Bettge A., Morris C., Blake T., Giroux M. (2002): Hordoindolines are associated with a major endosperm-texture QTL in barley (Hordeum vulgare). Genome, 45: 584–591.
Boros D. (2002): Physico-chemical quality indicators suitable in selection of triticale for high nutritive value. In: Arseniuk E. (ed.): Proc. 5th Int Triticale Symposium, Radzików, June 30–July 5, 2002: 239–244.
Chantret N., Salse J., Sabot F., Bellec A., Laubin B., Dubois I., Dossat C., Sourdille P., Joudrier P., Gautier M.-F., Cattolico L., Beckert M., Aubourg S., Weissenbach J., Caboche M., Leroy P., Bernard M., Chalhoub B. (2008): Contrasted microcolinearity and gene evolution within a homoeologous region of wheat and barley species. Journal of Molecular Evolution, 66: 138–150.
Darlington H., Rouster J., Hoffmann L., Halford N., Shewry P., Simpson D. (2001): Identification and molecular characterisation of hordoindolines from barley grain. Plant Molecular Biology, 47: 785–794.
Dennett A.L., Trethowan R.M. (2013): Milling efficiency of triticale grain for commercial flour production. Journal of Cereal Science, 57: 527–530.
Doxastakis G., Zafiriadis I., Irakli M., Marlani H., Tananaki C. (2002): Lupin, soya and triticale addition to wheat flour doughs and their effect on rheological properties. Food Chemistry, 77: 219–227.
Faměra O., Hrušková M., Novotná D. (2004): Evaluation of methods for wheat grain hardness determination. Plant Soil and Environment, 50: 489–493.
Gasparis S., Orczyk W., Nadolska-Orczyk A. (2013): Sina and Sinb genes in triticale do not determine grain hardness contrary to their orthologs Pina and Pinb in wheat. BMC Plant Biology, 13: 190.
Gautier M.F., Cosson P., Guirao A., Alary R., Joudrier P. (2000): Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species. Plant Science, 153: 81–91.
Gazza L., Taddei F., Conti S., Gazzelloni G., Muccilli V., Janni M., D’ovidio R., Alfieri M. Redaelli R., Pogna N.E. (2015): Biochemical and molecular characterization of Avena indolines and their role in kernel texture. Molecular Genetics and Genomics, 290: 39–54.
Giroux M.J., Morris C.F. (1997): A glycine to serine change in puroindoline b is associated with wheat grain hardness and low levels of starch-surface friabilin. Theoretical and Applied Genetics, 95: 857–864.
Gollan P., Smith K., Bhave M. (2007): Gsp-1 genes comprise a multigene family in wheat that exhibits a unique combination of sequence diversity yet conservation. Journal of Cereal Science, 45: 184–198.
Greenblatt G., Bettge A., Morris C. (1995): Relationship between endosperm texture and the occurrence of friabilin and bound polar lipids on wheat starch. Cereal Chemistry, 72: 172–176.
Greenwell P., Schofield J. (1986): A starch granule protein associated with endosperm softness in wheat. Cereal Chemistry, 63: 379–380.
Győri Z., Kruppa J., Ungai D., Mile I.G., Sipos P. (2009): Examination of technological and nutritional properties of breads made from triticale flour. In: Ugarčić-Hardi Z., Jukić M., Komlenić D.K., Planinić M., Obad L. (eds.): Proc. 5th Int. Congress Flour-Bread’09. 7th Croatian Congress of Cereal Technologists , Opatija, Oct 21–23, 2009: 503–507.
Hansen H.B., Møller B., Andersen S.B., Jørgensen J.R., Hansen Å. (2004): Grain characteristics, chemical composition, and functional properties of rye (Secale cereale L.) as influenced by genotype and harvest year. Journal of Agricultural and Food Chemistry, 52: 2282–2291.
Hrušková M., Švec I. (2009): Wheat hardness in relation to other quality factors. Czech Journal of Food Sciences, 27: 240–248.
Jing W., Demcoe A.R., Vogel H.J. (2003): Conformation of a bactericidal domain of puroindoline a: Structure and mechanism of action of a 13-residue antimicrobial peptide. Journal of Bacteriology, 185: 4938–4947.
Jolly C.J., Glenn G.M., Rahman S. (1996): Gsp-1 genes are linked to the grain hardness locus (Ha) on wheat chromosome 5D. Proceedings of the National Academy of Sciences, 93: 2408–2413.
Leon A., Rubiolo A., Anon M. (1996): Use of triticale flours in cookies: quality factors. Cereal Chemistry, 73: 779–784.
Li G., He Z., Peña R.J., Xia X., Lillemo M., Sun Q. (2006): Identification of novel secaloindoline-a and secaloindoline-b alleles in CIMMYT hexaploid triticale lines. Journal of Cereal Science, 43: 378–386.
Li G., Gao D., La S., Wang H., Li J., He W., Yang E., Yang Z. (2016): Characterization of wheat-Secale africanum chromosome 5R(a) derivatives carrying Secale specific genes for grain hardness. Planta, 243: 1203–1212.
Lillemo M., Simeone C.M., Morris C.F. (2002): Analysis of puroindoline a and b sequences from Triticum aestivum cv. 'Penawawa' and related diploid taxa. Euphytica, 126: 321–331.
Lukaszewski A.J. (2000): Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Science, 40: 216.
Lukaszewski A.J. (2006): Cytogenetically engineered rye chromosomes 1R to improve bread-making quality of hexaploid triticale. Crop Science, 46: 2183.
Massa A.N., Morris C.F. (2006): Molecular evolution of the puroindoline-a, puroindoline-b, and grain softness protein-1 genes in the tribe Triticeae. Journal of Molecular Evolution, 63: 526–536.
Massa A.N., Morris C.F., Gill B.S. (2004): Sequence diversity of puroindoline-a, puroindoline-b, and the grain softness protein genes in Aegilops tauschii Coss. Crop Science, 44: 1808.
Oliete B., Pérez G.T., Gómez M., Ribotta P.D., Moiraghi M., León A.E. (2010): Use of wheat, triticale and rye flours in layer cake production. International Journal of Food and Science Technology, 45: 697–706.
Oury F.X., Lasme P., Michelet C., Rousset M., Abecassis J., Lullien-Pellerin V. (2015): Relationships between wheat grain physical characteristics studied through near-isogenic lines with distinct puroindoline-b allele. Theoretical and Applied Genetics, 128: 913–929.
Peña R.J. (2004): Food uses of triticale. In: Mergoum M., Gómez-Macpherson H. (eds.): Triticale Improvement and Production. Rome, FAO: 37–48.
Ragupathy R., Cloutier S. (2008): Genome organisation and retrotransposon driven molecular evolution of the endosperm Hardness (Ha) locus in Triticum aestivum cv Glenlea. Molecular Genetics and Genomics, 280: 467–481.
Ramírez A., Pérez G.T., Ribotta P.D., León A.E. (2003): The occurrence of friabilins in triticale and their relationship with grain hardness and baking quality. Journal of Agricultural and Food Chemistry, 51: 7176–7181.
Simeone M.C., Lafiandra D. (2005): Isolation and characterisation of friabilin genes in rye. Journal of Cereal Science, 41: 115–122.
Tanchak M.A., Schernthaner J.P., Giband M., Altosaar I. (1998): Tryptophanins: isolation and molecular characterization of oat cDNA clones encoding proteins structurally related to puroindoline and wheat grain softness proteins. Plant Science, 137: 173–184.
Tranquilli G., Heaton J., Chicaiza O., Dubcovsky J. (2002): Substitutions and deletions of genes related to grain hardness in wheat and their effect on grain texture. Crop Science, 42: 1812–1817.
Trojan V., Musilová M., Vyhnánek T., Klejdus B., Hanáček P., Havel L. (2014): Chalcone synthase expression and pigment deposition in wheat with purple and blue colored caryopsis. Journal of Cereal Science, 59:48–55.
Wall M., Wheeler H., Huebsch M., Smith J., Figeys D., Altosaar I. (2010): The tryptophan-rich domain of puroindoline is directly associated with the starch granule surface as judged by tryptic shaving and mass spectrometry. Journal of Cereal Science, 52: 115–120.
Williams P.C. (1986): The influence of chromosome number and species on wheat hardness. Cereal Chemistry, 63: 56–58.
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