Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza Y., Chen X., Gan X., Yan Z., Mu D., Wang Q. (2018): Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza. Czech J. Genet. Plant Breed., 54: 177-182.
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Copalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In this study, we investigated the SmCPS1 gene promoter region together with the 388 bp downstream from the translation start site and the content of tanshinones of 12 different genotypes of Salvia miltiorrhiza. The cis-elements of SmCPS1 promoter were predicted and analysed by the Plant Transcriptional Regulatory Map database. We found (1) a different correlation between the polymorphism in the promoter region and the contents of tanshinones; (2) functional polymorphic loci – four tandem repeat variations, three indels and five single nucleotide polymorphisms (SNPs) in five cis-elements, three SNPs in exons and two SNPs in introns; (3) the correlation coefficient was higher when only functional (informative) polymorphic loci were considered. These findings have laid the foundation for further exploring the interspecific variation of S. miltiorrhiza and its relationship with the contents of tanshinones.

Cui Guanghong, Duan Lixin, Jin Baolong, Qian Jun, Xue Zheyong, Shen Guoan, Snyder John, Hugh, Song Jingyuan, Chen Shilin, Huang Luqi, Peters Reuben, J., Qi Xiaoquan (): Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza Bunge. Plant Physiology, , pp.00695.2015-
Guo J., Zhou Y. J., Hillwig M. L., Shen Y., Yang L., Wang Y., Zhang X., Liu W., Peters R. J., Chen X., Zhao Z. K., Huang L. (2013): CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts. Proceedings of the National Academy of Sciences, 110, 12108-12113
Jin J., Tian F., Yang D. C., Meng Y. Q., Kong L., Luo J., Gao G. (2017): PlantTFDB 4.0: toward a central hub for transcription factors and regulatory interactions in plants. Nucleic Acids Research, 45: D1040–D1045.
Kage Udaykumar, Kumar Arun, Dhokane Dhananjay, Karre Shailesh, Kushalappa Ajjamada C. (2015): Functional molecular markers for crop improvement. Critical Reviews in Biotechnology, 36, 917-930
Kumar Sudhir, Stecher Glen, Tamura Koichiro (2016): MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874
Le Hir Hervé, Nott Ajit, Moore Melissa J. (2003): How introns influence and enhance eukaryotic gene expression. Trends in Biochemical Sciences, 28, 215-220
Li Bin, Cui Guanghong, Shen Guoan, Zhan Zhilai, Huang Luqi, Chen Jiachun, Qi Xiaoquan (2017): Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza. Scientific Reports, 7, -
Li T., Fan H., Gao Z., Zhou Y., Yang H. (2008): Studies on genetic diversity of Salvia miltiorrhiza bunge with a molecular marker SRAP. Journal of Nuclear Agricultural Sciences, 22: 576–580.
Ma Yimian, Yuan Lichai, Wu Bin, Li Xian’en, Chen Shilin, Lu Shanfa (2012): Genome-wide identification and characterization of novel genes involved in terpenoid biosynthesis in Salvia miltiorrhiza. Journal of Experimental Botany, 63, 2809-2823
Nithianantharajah Jess, Hannan Anthony J. (2007): Dynamic mutations as digital genetic modulators of brain development, function and dysfunction. BioEssays, 29, 525-535
Peakall R., Smouse P. E. (2012): GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics, 28, 2537-2539
Pham T., Day S.M., Glassford W.J., Williams T.M., Rebeiz M. (2017): The evolutionary origination of a novel expression pattern through an extreme heterochronic shift. Evolution & Development, 19: 43–55.
Song Zhenqiao, Li Xingfeng, Wang Honggang, Wang Jianhua (2010): Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica, 138, 241-249
Stewart A. J., Hannenhalli S., Plotkin J. B. (2012): Why Transcription Factor Binding Sites Are Ten Nucleotides Long. Genetics, 192, 973-985
Wang W., Shan C., Ni D., Wang Z. (2010): SRAP – Analysis of genetic diversity about germplasm in Salvia miltiorrhiza from different sources. Chinese Traditional and Herbal Drugs, 41: 632–635.
Wittkopp Patricia J., Kalay Gizem (2012): Cis-regulatory elements: molecular mechanisms and evolutionary processes underlying divergence. Nature Reviews Genetics, 13, 59-69
Yan X. (2015): Dan Shen (Salvia miltiorrhiza) in Medicine. Beijing, Springer Netherlands and People’s Medical Publishing House.
Yang L., Ding G., Lin H., Cheng H., Kong Y., Wei Y., Fang X., Liu R., Wang L., Chen X., Yang C. (2013): Transcriptome analysis of medicinal plant Salvia miltiorrhiza and identification of genes related to tanshinone biosynthesis. PLoS ONE, 8: e80464.
Zeng Huiting, Su Shulan, Xiang Xiang, Sha Xiuxiu, Zhu Zhenhua, Wang Yanyan, Guo Sheng, Yan Hui, Qian Dawei, Duan Jinao (2017): Comparative Analysis of the Major Chemical Constituents in Salvia miltiorrhiza Roots, Stems, Leaves and Flowers during Different Growth Periods by UPLC-TQ-MS/MS and HPLC-ELSD Methods. Molecules, 22, 771-
Zhang Yuan, Li Xing, Wang Zhezhi (2013): Diversity Evaluation of Salvia miltiorrhiza Using ISSR Markers. Biochemical Genetics, 51, 707-721
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