Genome-wide identification and analysis of the MLO gene families in three Cucurbita species

Zhu L., Li Y., Li J., Wang Y., Zhang Z., Wang Y., Wang Z., Hu J., Yang L., Sun S. (2021): Genome-wide identification and analysis of the MLO gene families in three Cucurbita species. Czech J. Genet. Plant Breed., 57: 119123.

supplementary materialdownload PDF

Powdery mildew (PM) is a major fungal disease in the Cucurbita species in the world, which can cause significant yield loss. The Mildew Locus O (MLO) family genes play important roles in the PM stress response. In this paper, twenty, twenty-one, and eighteen candidate MLO genes in Cucurbita moschata, Cucurbita maxima and Cucurbita pepo, respectively, were identified and designated as CmoMLO, CmaMLO and CpeMLO, respectively. The phylogenetic analysis indicated that these MLOs were divided into five clades and the number of MLOs belonging to clade V in the Cucurbita species was more than that in other crops. Furthermore, the expression analysis in the susceptibility (S) and resistance (R) lines showed that CpeMLO1, CpeMLO2 and CpeMLO5 might be involved in the susceptibility response. CpeMLO4 and CpeMLO6 showing opposite expression patterns in the R/S lines might be involved in the resistance response. All these data would be beneficial for future functional analysis of MLOs in the Cucurbita species.

Andolfo G., Iovieno P., Ricciardi L., Lotti C., Filippone E., Pavan S., Ercolano M.R. (2019): Evolutionary conservation of MLO gene promoter signatures. BMC Plant Biology, 19: 150.
Appiano M., Catalano D., Martinez M.S, Lotti C., Zheng Z., Visser R.G.F., Ricciardi L., Bai Y.L., Pavan S. (2015): Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features. BMC Plant Biology, 15: 257.
Berg J.A., Appiano M., Bijsterbosch G., Visser R.G.F., Schouten H.J., Bai Y.L. (2017): Functional characterization of cucumber (Cucumis sativus L.) Clade V MLO genes. BMC Plant Biology, 17: 80.
Consonni C., Humphry M.E., Hartmann H.A., Livaja M., Durner J., Westphal L., Vogel J., LipkaV., Kemmerling B., Schulze-Lefert P., Somerville S.C., Panstruga R. (2006): Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nature Genetics, 38: 716–720.
Inuma T., Khodaparast S.A., Takamatsu S. (2007): Multilocus phylogenetic analyses within Blumeria graminis, a powdery mildew fungus of cereals. Molecular Phylogenetics and Evolution, 44: 741–751.
Iovieno P., Andolfo G., Schiavulli A., Catalano D., Ricciardi L., Frusciante L., Ercolano M.R., Pavan S. (2015): Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp. BMC Genomics, 16: 1112.
Montero-Pau J., Blanca J., Bombarely A., Ziarsolo P., Este-ras C., Marti-Gomez C., Ferriol M., Gomez P., Jamilena M., Mueller L., Pico B., Canizares J. (2018): De novo assembly of the zucchini genome reveals a whole-genome duplication associated with the origin of the Cucurbita genus. Plant Biotechnology Journal, 16: 1161–1171.
Livak K.J., Schmittgen T.D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 25: 402–408.
Pessina S., Lenzi L., Perazzolli M., Campa M., Dalla Costa L., Urso S., Vale G., Salamini F., Velasco R., Malnoy M. (2016): Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine. Horticulture Research, 3: 16016.
Sedlarova M., Lebeda A., Miksikova P., Duchoslav M., Sedlakova B., McCreight J.D. (2009): Histological aspects of Cucumis melo PI 313970 resistance to Podosphaera xanthii and Golovinomyces cichoracearum. Journal of Plant Diseases and Protection, 116: 169–176.
Seifi A., Gao D.L., Zheng Z., Pavan S., Faino L., Visser R.G.F., Wolters A.M.A., Bai Y.L. (2014): Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives. European Journal of Plant Pathology, 138: 641–665.
Vakalounakis D.J., Klironomou E., Papadakis A. (2010): Species spectrum, host range and distribution of powdery mildews on Cucurbitaceae in Crete. Plant Pathology, 43: 813–818.
Van Schie C.C.N., Takken F.L.W. (2014): Susceptibility genes 101: How to be a good host. Annual Review of Phytopathology, 52: 551–581.
Zheng Z., Appiano M., Pavan S., Bracuto V., Ricciardi L., Visser R.G., Wolters A.M., Bai Y. (2016): Genome-wide study of the tomato SlMLO gene family and its functional characterization in response to the powdery mildew fungus Oidium neolycopersici. Frontiers in Plant Science, 7: 380.
Zhu J.H., Wang J.M., Jia Q.J., Yang J.M., Zhou Y.J., Lin F., Hua W., Shang Y. (2010): Pathotypes and genetic diversity of Blumeria graminis f.sp. hordei in the winter barley regions in China. Agricultural Sciences in China, 9: 1787–1798.
supplementary materialdownload PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti