Evaluation and Mapping of a Leaf Rust Resistance Gene Derived from Hordeum vulgare subsp. spontaneum
D. Kopahnke, M. Nachtigal, F. Ordon, J. Steffenson Bhttps://doi.org/10.17221/3704-CJGPBCitation:Kopahnke D., Nachtigal M., Ordon F., Steffenson B J. (2004): Evaluation and Mapping of a Leaf Rust Resistance Gene Derived from Hordeum vulgare subsp. spontaneum. Czech J. Genet. Plant Breed., 40: 86-90.
Studies of marker development were performed on a doubled haploid population derived from the cross of a highly resistant line H. spontaneum 677 × Krona (susceptible). Previous segregation studies on F2 and F3 populations revealed that the resistance of H. spontaneum 677 was likely due to a single dominant gene. Bulked segregant analysis using AFLPs and SSRs was conducted to identify markers linked to this leaf rust resistance gene. By this approach the resistance gene was located on barley chromosome 2H with the closest markers linked at 6.1 cM (E35M54b) and 13.6 cM (Bmac0218) based on the analysis of 83 DH-lines. In order to get first hints whether this gene may be allelic to rph16 located on chromosome 2H STS marker MWG 2133 co-segregating with rph16 was tested but it turned out to be monomorphic. However, in a resistance test with a set of four different isolates of Puccinia hordei, H. spontaneum 677 showed a different reaction pattern from that of H. spontaneum 680, the source of rph16. Tests of allelism to confirm these results are in progress.
Hordeum vulgare; barley; Puccinia hordei; leaf rust; resistance; SSRs; AFLPs; genetic mapping