Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe
A. Lisek, E. Rozpara, A. Głowacka, D. Kucharska, M. Zawadzkahttps://doi.org/10.17221/103/2014-HORTSCICitation:Lisek A., Rozpara E., Głowacka A., Kucharska D., Zawadzka M. (2015): Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe. Hort. Sci. (Prague), 42: 13-21.
Sweet cherry is a species that is characterized by self-incompatibility, which causes difficulties in obtaining high yields of sweet cherry fruit. Determination of the composition of the S-alleles of sweet cherry cultivars is useful both to growers producing the fruit and breeders when selecting cultivars for cross-fertilizations. In this work, S-alleles have been identified for 47 sweet cherry cultivars from Central and Eastern Europe, mostly from Ukraine and the Czech Republic, with S-genotypes of 43 cultivars identified for the first time. To identify the S-alleles, PCR-based methods were used. In each cultivar, two of the following eight S-alleles were identified: S1, S2, S3, S4, S5, S6, S9 and S13. In the cultivars from the Czech Republic, the most frequently occurring were the alleles S3, S1 and S4. In the cultivars originating in Ukraine, the most frequently found were the alleles S5 and S9, which makes them differ considerably from the sweet cherry cultivars from other regions of Europe. The tested sweet cherry cultivars were assigned to 20 of the existing incompatibility groups.Keywords:
Prunus avium L.; self-incompatibility; S-RNase; incompatibility groups; allele frequencyReferences:
Békefi Z., Tobutt K.R., Sonneveld T. (2003): Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods. International Journal of Horticultural Science, 9: 37–42.Békefi Z., Vaughan S. P., Tobutt K. R. (2010): Determination of incompatibility <i>(S)</i> genotypes of sweet cherries in the Hungarian gene-bank by a PCR-based method.. Acta Agronomica Hungarica, 58, 377-384 https://doi.org/10.1556/AAgr.58.2010.4.6Bošković R., Russel K., Tobutt K.R. (1997): Inheritance of stylar ribonucleases in cherry progenies, and reassignment of incompatibility alleles to two incompatibility groups. 1997. Euphytica, 95: 221–228. https://doi.org/10.1023/A:1002945529157Boˇskovi´c R., Tobutt K. R. (2001): Genotyping cherry cultivars assigned to incompatibility groups, by analysing stylar ribonucleases. TAG Theoretical and Applied Genetics, 103, 475-485 https://doi.org/10.1007/PL00002906Cachi Ariana M., Wünsch Ana (2014): S-genotyping of sweet cherry varieties from Spain and S-locus diversity in Europe. Euphytica, 197, 229-236 https://doi.org/10.1007/s10681-014-1061-0Choi Ch., Tao R., Andersen R.L. (2002): Identification of self-incompatibility alleles and pollen incompatibility groups in sweet cherry by PCR based S-allele typing and controlled pollination. Euphytica, 123: 9–20. https://doi.org/10.1023/A:1014403802677De Cuyper B., Sonneveld T., Tobutt K.R. (2005): Determining self-incompatibility genotypes in Belgian wild cherries.. Molecular Ecology, 14, 945-955 https://doi.org/10.1111/j.1365-294X.2005.02460.xDe Nettancourt D. (1984): Incompatibility. In: Cellular Interactions. Berlin Heidelberg, Springer: 624–639.Ercisli S., Radunic M., Gadze J., Ipek A., Skaljac M., Cmelik Z. (2012). S-RNase based S-genotyping of Croatian sweet cherry (Prunus avium L.) genotypes. Scientia Horticulturae, 139: 21–24.FAOSTAT (2012): Available at http://faostat3.fao.org/home/index.html#VISUALIZEGanopoulos I.V., Argiriou A., Tsaftaris A.S. (2010): Determination of self-incompatible genotypes in 21 cultivated sweet cherry cultivars in Greece and implications for orchard cultivation. Journal of Horticultural Science and Biotechnology, 85: 444–448.Gisbert A.D., Badenes M.L., Tobutt K.R., Llacer G., Romero C. (2008): Determination of the S-allele composition of sweet cherry (Prunus avium L.) cultivars grown in the southeast of Spain by PCR analysis. Journal of Horticultural Science and Biotechnology, 83: 246–252.Hauck N.R., Iezzoni A.F., Yamane H., Tao R. (2001): Revisiting the S-allele nomenclature in sweet cherry (Prunus avium) using RFLP profiles. Journal of the American Society for Horticultural Science, 126: 654–660.Ipek A., Gulen H., Ipek M., Akcay M.E., Ergin S., Eris A. (2011): Determination of self-incompatibility groups of sweet cherry genotypes from Turkey. Genetics and Molecular Research, 10, 253-260 https://doi.org/10.4238/vol10-1gmr1024Lacis Gunars, Kaufmane Edite, Rashal Isaak, Trajkovski Viktor, Iezzoni Amy F. (2008): Identification of self-incompatibility (S) alleles in Latvian and Swedish sweet cherry genetic resources collections by PCR based typing. Euphytica, 160, 155-163 https://doi.org/10.1007/s10681-007-9496-1Marchese A., Tobutt K.R., Raimondo A., Motisi A., Bošković R.I., Clarke J., Caruso T. (2007): Morphological characteristics, microsatellite fingerprinting and determination of incompatibility genotypes of Sicilian sweet cherry cultivars. Journal of Horticultural Science and Biotechnology, 82: 41–48.Schuster M., Flachowsky H., Köhler D. (2007): Determination of self-incompatible genotypes in sweet cherry (Prunus avium L.) accessions and cultivars of the German Fruit Gene Bank and from private collections. Plant Breeding, 126, 533-540 https://doi.org/10.1111/j.1439-0523.2007.01401.xSchuster Mirko (2012): Incompatible (S-) genotypes of sweet cherry cultivars (Prunus avium L.). Scientia Horticulturae, 148, 59-73 https://doi.org/10.1016/j.scienta.2012.09.012Sonneveld T., Robbins T. P., Bošković R., Tobutt K. R. (2001): Cloning of six cherry self-incompatibility alleles and development of allele-specific PCR detection. TAG Theoretical and Applied Genetics, 102, 1046-1055 https://doi.org/10.1007/s001220000525Sonneveld T., Tobutt K. R., Robbins T. P. (2003): Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers. TAG Theoretical and Applied Genetics, 107, 1059-1070 https://doi.org/10.1007/s00122-003-1274-4Sonneveld T., Robbins T. P., Tobutt K. R. (2006): Improved discrimination of self-incompatibility S-RNase alleles in cherry and high throughput genotyping by automated sizing of first intron polymerase chain reaction products. Plant Breeding, 125, 305-307 https://doi.org/10.1111/j.1439-0523.2006.01205.xSzikriszt B., Doğan A., Ercisli S., Akcay M. E., Hegedűs A., Halász J. (2013): Molecular typing of the self-incompatibility locus of Turkish sweet cherry genotypes reflects phylogenetic relationships among cherries and other Prunus species. Tree Genetics & Genomes, 9, 155-165 https://doi.org/10.1007/s11295-012-0543-2Tobutt K.R., Sonneveld T., Békefi Z., Bošković R. (2004): Cherry (in)compatibility genotypes – an updated cultivar table. Acta Horticulturae (ISHS), 663: 667–671.Ushijima K. (): Structural and Transcriptional Analysis of the Self-Incompatibility Locus of Almond: Identification of a Pollen-Expressed F-Box Gene with Haplotype-Specific Polymorphism. THE PLANT CELL ONLINE, 15, 771-781 https://doi.org/10.1105/tpc.009290Vaughan S. P., Bošković R. I., Gisbert-Climent A., Russell K., Tobutt K. R. (2008): Characterisation of novel S-alleles from cherry (Prunus avium L.). Tree Genetics & Genomes, 4, 531-541 https://doi.org/10.1007/s11295-007-0129-6Wiersma P. A., Wu Z., Zhou L., Hampson C., Kappel F. (2001): Identification of new self-incompatibility alleles in sweet cherry ( Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis. TAG Theoretical and Applied Genetics, 102, 700-708 https://doi.org/10.1007/s001220051700Wunsch A., Hormaza J. I. (2004): S-allele identification by PCR analysis in sweet cherry cultivars. Plant Breeding, 123, 327-331 https://doi.org/10.1111/j.1439-0523.2004.00984.xYamane H. (2003): A Pollen-Expressed Gene for a Novel Protein with an F-box Motif that is Very Tightly Linked to a Gene for S-RNase in Two Species of Cherry, Prunus cerasus and P. avium. Plant and Cell Physiology, 44, 764-769 https://doi.org/10.1093/pcp/pcg088