Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication
J. Sedlák, F. Papršteinhttps://doi.org/10.17221/115/2010-HORTSCICitation:Sedlák J., Paprštein F. (2011): Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication. Hort. Sci. (Prague), 38: 159-162.
The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson’s rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with the concentration 1 mg/l of zeatin. The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, auxin NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation.Keywords:
in vitro; multiplication; rooting; explant; Howes