Effects of garlic genotype on cloves formation under in vitro conditions

https://doi.org/10.17221/151/2015-HORTSCICitation:Fišerová H., Vyhnánek T., Staňková Z., Kozák V., Klemš M., Havel L. (2016): Effects of garlic genotype on cloves formation under in vitro conditions. Hort. Sci. (Prague), 43: 142-148.
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Prior to the transfer of multiplicated garlic plants from in vitro to ex vitro conditions it is necessary to induce the formation of bulbs and to verify the identity of propagated young individuals with their maternal plant. This study deals with effects of exogenous compounds (i.e. paclobutrazol, abscisic acid and ethylene) on formation of bulbs of four garlic cultivars (Lan, Lukan, Emilie, Japo) and on production of ethylene and carbon dioxide (CO2). In the softneck garlic cv. Emilie, the exogenous application of paclobutrazol increased numbers of daughter cloves and production of both ethylene and CO2. In the softneck cv. Lukan the formation of cloves was higher after the application of ABA than after the application of paclobutrazol (PP 333). An exogenous application of ethylene inhibited the formation of bulbs. Analyses of DNA polymorphism, performed by means of microsatellite markers, verified the identity of bulbs produced under in vitro conditions with their mother plants. The hardneck cv. Lan differed form softneck cvs Lukan, Emilie and Japo.
BIRAN I., LESHEM B., GUR I., HALEVY A. H. (1974): Further Studies on the Relationship between Growth Regulators and Tuberization of Dahlias. Physiologia Plantarum, 31, 23-28  https://doi.org/10.1111/j.1399-3054.1974.tb03672.x
Burg S. P., Burg E. A. (1968): Ethylene Formation in Pea Seedlings; Its Relation to the Inhibition of Bud Growth Caused by Indole-3-Acetic Acid. PLANT PHYSIOLOGY, 43, 1069-1074  https://doi.org/10.1104/pp.43.7.1069
Cvikrová M., Sukhova L.S., Eder J., Korableva N.P. (1994): Possible involvement of abscisic acid, ethylene and phenolic acids in potato tuber dormancy. Plant Physiology and Biochemistry, 32: 685–691. 
(1991): Davis, T. D., Haissig, B. E., Sankhla, N. (ed.): Adventitious Root Formation in Cuttings. Advances in Plant Sciences Series. Volume 2. Advances in Plant Sciences Series. Volume 2. Biologia Plantarum, 33, 114-114  https://doi.org/10.1007/BF02897787
De Mattia Fabrizio, Imazio Serena, Grassi Fabrizio, Lovicu Gianni, Tardáguila Javier, Failla Osvaldo, Maitt Chiara, Scienza Attilio, Labra Massimo (2016): Genetic characterization of Sardinia grapevine cultivars by SSR markers analysis. OENO One, 41, 175-  https://doi.org/10.20870/oeno-one.2007.41.4.837
England D.J.F. (1978): PP 333 – A new grass retardant. In: I. H. Rorison and R. Hunt (eds): Amenity Grassland: An Ecological Perspective. Chichester, Wiley.
Fišerová H., Kula E., Klemš M., Reinöhl V. (2001): Phytohormones as indicators of the degree of damage in birch. Biológia, 56: 405–409.
Fišerová H., Klemš M., Čepl J., Macháčková I.,Procházka S. (2004): Dynamics of ethylene and abscisic acid levels during the growth of potato tubers. Acta Physiologie Plantarum, 26: 18.
Fišerová H., Mikušová Z., Klemš M. (2008): Estimation of
ethylene production and 1-aminocyclopropane-1-carbo-xylic acid content in plants by means of gas chromatography. Plant, Soil and Environment, 54: 55–60.
FIŠEROVÁ HELENA, PROKEŠ JOSEF, HELÁNOVÁ ALENA, HARTMANN JIŘÍ (2010): Malt quality during the post-harvest maturation of barley.. Kvasny Prumysl, 56, 93-99  https://doi.org/10.18832/kp2010012
Hampl V., Pavlicek A., Flegr J. (): Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 51, 731-735  https://doi.org/10.1099/00207713-51-3-731
Haque M., Wada T., Hattori K. (2000): GARLIC ROOTS FOR MICROPROPAGATION THROUGH IN VITRO BULBLET FORMATION. Acta Horticulturae, , 45-52  https://doi.org/10.17660/ActaHortic.2000.520.2
Havel L., Novák F.J. (1988): Regulation of Somatic Embryogenesis and Organogenesis in Allium carinatum L.. Journal of Plant Physiology, 132, 373-377  https://doi.org/10.1016/S0176-1617(88)80123-8
Hradilík J., Fišerová H. (1986): Interakce mezi giberelinem (GA3) a paclobutrazolem (P 333) u salátu, hrachu a lnu. Acta Universitatis Agriculturae at Silviculturae (Brno), XXXV: 5–11.
Hradilík J., Fišerová H. (1987): Vliv kyseliny giberelové (GA3) a paclobutrazolu (PP 333) na morfologii bramboříku. Acta Universitatis Agriculturae at Silviculturae (Brno), XXXV: 23–26.
Jaccard P. (1908): Nouvelles recherches sur la distribution florale. Bulletin del la Societé Vaudoise des Scientes Naturelles, 44: 223–270.
Králík J., Šebánek J. (1989): On the stimulating effects of paclobutrazol on the rhizogenezis of Ligustrum vulgare L. cuttings. Acta Universitatis Agriculturae at Silviculturae (Brno), 37: 51–54.
Li Sha Liu, Ren Li, Yongqin Zhao, Chang Long Wen, Shuxin Ren, Yang Dong Guo (2011): High efficiency regeneration and genetic stability analysis of somatic clones of Gynura bicolor DC.. African Journal of Biotechnology, 10, 10380-10386  https://doi.org/10.5897/AJB10.1790
Ma K.H., Kwag J.G., Zhao W., Dixit A., Lee G.A., Kim H.H., Chung I.M., Kim N.S., Lee J.S., Ji J.J., Kim T.S., Park Y.J. (2009): Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic
(Allium sativum L.). Scientia Horticulturae, 122: 355–361.
Macháčková I., Ullmann J. (1987): Ethylene – a natural regulator of plant growth and development. Biologické listy, 52: 282–306.
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nagakubo Takayuki, Nagasawa Akitsu, Ohkawa Hideo (1993): Micropropagation of garlic through in vitro bulblet formation. Plant Cell, Tissue and Organ Culture, 32, 175-183  https://doi.org/10.1007/BF00029840
Nambara Eiji, Okamoto Masanori, Tatematsu Kiyoshi, Yano Ryoichi, Seo Mitsunori, Kamiya Yuji (2010): Abscisic acid and the control of seed dormancy and germination. Seed Science Research, 20, 55-  https://doi.org/10.1017/S0960258510000012
Novák F. J., Havel L. (1981): Shoot production fromin vitro cultured flower heads ofAllium porrum L. Biologia Plantarum, 23, 266-269  https://doi.org/10.1007/BF02895362
Ovesná J., Leišová-Svobodová L., Kučera L. (2014): Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic. Czech Journal of Genetics and Plant Breeding, 50: 226–234.
Page R.D.M. (1996): Treeview: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12: 357–358.
Phulwaria Mahendra, Rai Manoj K., Shekhawat N. S. (2013): An Improved Micropropagation of Arnebia hispidissima (Lehm.) DC. and Assessment of Genetic Fidelity of Micropropagated Plants Using DNA-Based Molecular Markers. Applied Biochemistry and Biotechnology, 170, 1163-1173  https://doi.org/10.1007/s12010-013-0266-3
PROKEŠ JOSEF, FIŠEROVÁ HELENA, HELÁNOVÁ ALENA, HARTMANN JIŘÍ (2006): Role of carbon dioxide and ethylene during storage process.. Kvasny Prumysl, 52, 349-352  https://doi.org/10.18832/kp2006029
Ridge Irene, Amarasinghe Ivan (1984): Ethylene and growth control in the fringed waterlily (Nymphoides peltata): Stimulation of cell division and interaction with buoyant tension in petioles. Plant Growth Regulation, 2, 235-249  https://doi.org/10.1007/BF00124772
Russell Joanne, Fuller John, Young George, Thomas Bill, Macaulay Malcolm, Waugh Robbie, Powell Wayne, Taramino Graziana (1997): Discriminating between barley genotypes using microsatellite markers. Genome, 40, 442-450  https://doi.org/10.1139/g97-059
Sohn E.Y., Kim Y.H., Kim J.T., Jang S.W., Lee I.J. (2011): Changes in endogenous abscisic acid, jasmonic acid and sucrose content during the bulb development in the cold-type cultivar of garlic (Allium sativum L.) of Korea. Korean Journal of Horticultural Science & Technology, 29: 1–9.
Šebánek J., Klíčová S., Králík J., Psota V., Vítková H., Kudová D., Reinöhl V. (1991): The Effect of paclobutrazol on the Level of endogenous IAA in Relation to the Rooting of Cuttings and Abscission of Petioles. Biochemie und Physiologie der Pflanzen, 187, 89-94  https://doi.org/10.1016/S0015-3796(11)80195-2
Vyhnánek T., Nevrtalová E., Slezáková K. (2009): Detection of the genetic variability of triticale using wheat and Rye SSR markers. Cereal Research Communications, 37, 23-29  https://doi.org/10.1556/CRC.37.2009.1.3
Yang S.F. (1980): Regulation of ethylene biosynthesis. Horticulturae Science. 15: 238–243.
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