Expression analysis of NAC genes during the growth and ripening of apples

https://doi.org/10.17221/153/2016-HORTSCICitation:Zhang Q., Li T., Zhang L., Dong W., Wang A. (2018): Expression analysis of NAC genes during the growth and ripening of apples. Hort. Sci. (Prague), 45: 1-10.
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Plant-specific NAC transcription factors (TFs) play crucial roles in various pathways related to the stress response. However, to date, little information regarding NAC gene regulation during fruit ripening is available for the apple (Malus domestica). Here, we report that 13 out of 182 MdNAC genes were differentially expressed during the stages of fruit growth and ripening. Sequence analysis indicates that these 13 MdNAC genes harbour distinct structures and potentially diverse functions. The expression of both MdNAC1a and MdNAC78 was repressed by ethylene and induced by 1-MCP during storage. MdNAC2, MdNAC26, MdNAC41, MdNAC57, MdNAC80, MdNAC91, MdNAC119 and MdNAC141 were up-regulated by ethylene and their transcription mirrored ethylene production rates during storage. MdNAC1, MdNAC16 and MdNAC32 did not respond to 1-MCP exposure. Additionally, the 13 MdNAC genes identified displayed differential tissue-specific expression patterns. These results suggest that NAC TFs play an important role in the regulation of apple development via both ethylene-dependent and -independent mechanisms.
References:
Aida M. (): Genes Involved in Organ Separation in Arabidopsis: An Analysis of the cup-shaped cotyledon Mutant. THE PLANT CELL ONLINE, 9, 841-857 https://doi.org/10.1105/tpc.9.6.841
 
Altschul S. (): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402 https://doi.org/10.1093/nar/25.17.3389
 
Bapat V.A, Trivedi P.K, Ghosh A, Sane V.A, Ganapathi T.R, Nath P. (2010): Ripening of fleshy fruit, molecular insight and the role of ethylene. Biotechnology Advances, 28: 107.
 
Berger Y., Harpaz-Saad S., Brand A., Melnik H., Sirding N., Alvarez J. P., Zinder M., Samach A., Eshed Y., Ori N. (2009): The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves. Development, 136, 823-832 https://doi.org/10.1242/dev.031625
 
Christianson J.A., Dennis E.S., Llewellyn D.J., Wilson I.W. (2010): ATAF NAC transcription factors: regulators of plant stress signaling. Plant Signaling & Behavior, 5: 428–432.
 
Gasic Ksenija, Hernandez Alvaro, Korban Schuyler S. (2004): RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Molecular Biology Reporter, 22, 437-438 https://doi.org/10.1007/BF02772687
 
Hao Yu-Jun, Song Qing-Xin, Chen Hao-Wei, Zou Hong-Feng, Wei Wei, Kang Xu-Sheng, Ma Biao, Zhang Wan-Ke, Zhang Jin-Song, Chen Shou-Yi (2010): Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta, 232, 1033-1043 https://doi.org/10.1007/s00425-010-1238-2
 
Hao Yu-Jun, Wei Wei, Song Qing-Xin, Chen Hao-Wei, Zhang Yu-Qin, Wang Fang, Zou Hong-Feng, Lei Gang, Tian Ai-Guo, Zhang Wan-Ke, Ma Biao, Zhang Jin-Song, Chen Shou-Yi (2011): Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants. The Plant Journal, 68, 302-313 https://doi.org/10.1111/j.1365-313X.2011.04687.x
 
He Xin-Jian, Mu Rui-Ling, Cao Wan-Hong, Zhang Zhi-Gang, Zhang Jin-Song, Chen Shou-Yi (2005): AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. The Plant Journal, 44, 903-916 https://doi.org/10.1111/j.1365-313X.2005.02575.x
 
Fan Jing, Gao Xue, Yang Ying-Wu, Deng Wei, Li Zheng-Guo (2007): Molecular Cloning and Characterization of a NAC-like Gene in “Navel” Orange Fruit Response to Postharvest Stresses. Plant Molecular Biology Reporter, 25, 145-153 https://doi.org/10.1007/s11105-007-0016-1
 
Kikuchi K., Ueguchi-Tanaka M., Yoshida K. T., Nagato Y., Matsusoka M., Hirano H. -Y. (2000): Molecular analysis of the NAC gene family in rice. MGG - Molecular and General Genetics, 262, 1047-1051 https://doi.org/10.1007/PL00008647
 
Kou Xiaohong, Watkins Christopher B., Gan Su-Sheng (2012): Arabidopsis AtNAP regulates fruit senescence. Journal of Experimental Botany, 63, 6139-6147 https://doi.org/10.1093/jxb/ers266
 
Liu Yong-Zhong, Baig M. N. R., Fan Rui, Ye Jun-Li, Cao Yin-Chuan, Deng Xiu-Xin (2009): Identification and Expression Pattern of a Novel NAM, ATAF, and CUC-Like Gene from Citrus sinensis Osbeck. Plant Molecular Biology Reporter, 27, 292-297 https://doi.org/10.1007/s11105-008-0082-z
 
Livak Kenneth J., Schmittgen Thomas D. (2001): Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25, 402-408 https://doi.org/10.1006/meth.2001.1262
 
Mao Xinguo, Zhang Hongying, Qian Xueya, Li Ang, Zhao Guangyao, Jing Ruilian (2012): TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. Journal of Experimental Botany, 63, 2933-2946 https://doi.org/10.1093/jxb/err462
 
Martel C., Vrebalov J., Tafelmeyer P., Giovannoni J. J. (2011): The Tomato MADS-Box Transcription Factor RIPENING INHIBITOR Interacts with Promoters Involved in Numerous Ripening Processes in a COLORLESS NONRIPENING-Dependent Manner. PLANT PHYSIOLOGY, 157, 1568-1579 https://doi.org/10.1104/pp.111.181107
 
Ma N.N., Feng H.L., Meng X., Li D., Yang D.Y., Wu C.G., Meng Q.W. (2014): Overexpression of tomato SlNAC1 transcription factor alters fruit pigmentation and softening. Plant Biology, 14: 351–360.
 
Shan Wei, Kuang Jian-fei, Chen Lei, Xie Hui, Peng Huan-huan, Xiao Yun-yi, Li Xue-ping, Chen Wei-xin, He Quan-guang, Chen Jian-ye, Lu Wang-jin (2012): Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening. Journal of Experimental Botany, 63, 5171-5187 https://doi.org/10.1093/jxb/ers178
 
Sharp R. E. (2004): Root growth maintenance during water deficits: physiology to functional genomics. Journal of Experimental Botany, 55, 2343-2351 https://doi.org/10.1093/jxb/erh276
 
Sisler E.C., Serek M., Dupilie E., Goren R. (1999): Inhibition of ethylene responses by 1-Methylcyclopropene and 3-Methylcyclopropene. Plant Growth Regulation, 27: 105–111.https://doi.org/10.1023/A:1006153016409
 
Souer Erik, van Houwelingen Adèle, Kloos Daisy, Mol Jos, Koes Ronald (1996): The No Apical Meristem Gene of Petunia Is Required for Pattern Formation in Embryos and Flowers and Is Expressed at Meristem and Primordia Boundaries. Cell, 85, 159-170 https://doi.org/10.1016/S0092-8674(00)81093-4
 
Su Hongyan, Zhang Shizhong, Yuan Xiaowei, Chen Changtian, Wang Xiao-Fei, Hao Yu-Jin (2013): Genome-wide analysis and identification of stress-responsive genes of the NAM–ATAF1,2–CUC2 transcription factor family in apple. Plant Physiology and Biochemistry, 71, 11-21 https://doi.org/10.1016/j.plaphy.2013.06.022
 
Zhu Mingku, Chen Guoping, Zhou Shuang, Tu Yun, Wang Yi, Dong Tingting, Hu Zongli (2014): A New Tomato NAC (NAM/ATAF1/2/CUC2) Transcription Factor, SlNAC4, Functions as a Positive Regulator of Fruit Ripening and Carotenoid Accumulation. Plant and Cell Physiology, 55, 119-135 https://doi.org/10.1093/pcp/pct162
 
Toivonen P.M.A., Lu C.W. (2005): Studies on elevated temperature, short-term storage of ‘Sunrise’ Summer apples using 1-MCP to maintain quality. Journal of Horticultural Science & Biotechnology, 80: 439–446.
 
Janssen Bart J, Thodey Kate, Schaffer Robert J, Alba Rob, Balakrishnan Lena, Bishop Rebecca, Bowen Judith H, Crowhurst Ross N, Gleave Andrew P, Ledger Susan, McArtney Steve, Pichler Franz B, Snowden Kimberley C, Ward Shayna (2008): Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biology, 8, 16- https://doi.org/10.1186/1471-2229-8-16
 
Wang A., Xu K.N. (2012): Characterization of two orthologs of Reversion-To-Ethylene Sensitivity1 in apple. Journal of Molecular Biology Research, 2(1).
 
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