In vitro storage of plum germplasm by slow growth

https://doi.org/10.17221/186/2014-HORTSCICitation:Gianní S., Sottile F. (2015): In vitro storage of plum germplasm by slow growth. Hort. Sci. (Prague), 42: 61-69.
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In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. – two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage.
References:
Ahmed M., Anjum M.A., Ahmed M.J., Sjid G.M., Yoqub A., Shafqat M. (2011): Role of plant growth regulators in preservation of Pyrus germplasm in vitro. African Journal of Biotechnology, 10: 14029–14037.
 
Bailey L.H., Bailey E.Z. (1976): Prunus L. In: A Concise Dictionary of Plants Cultivated in the United States and Canada. New York, Macmillan: 918–921.
 
Bairu Michael W., Stirk Wendy A., Van Staden Johannes (2009): Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell, Tissue and Organ Culture (PCTOC), 98, 239-248  https://doi.org/10.1007/s11240-009-9560-8
 
Biswal U. C., Biswal Basanti (1984): PHOTOCONTROL OF LEAF SENESCENCE. Photochemistry and Photobiology, 39, 875-879  https://doi.org/10.1111/j.1751-1097.1984.tb08874.x
 
Druart P. (1985): In vitro germplasm preservation techniques for fruits trees. In: Schäfer-Menuhr A. (ed.): In Vitro Techniques: Propagation and Long-term Storage. Dordrecht, Martinus Nijhoff/Dr W Junk for CEC: 167–171.
 
Engelmann F. (2011): Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology-Plant, 45: 5–16.
 
Gao Xin, Alvo Mayer, Chen Jie, Li Gang (2008): Nonparametric multiple comparison procedures for unbalanced one-way factorial designs. Journal of Statistical Planning and Inference, 138, 2574-2591  https://doi.org/10.1016/j.jspi.2007.10.015
 
Hausman Jean-Francois, Neys Olivier, Kevers Claire, Gaspar Thomas (1994): Effect of in vitro storage at 4 �C on survival and proliferation of poplar shoots. Plant Cell, Tissue and Organ Culture, 38, 65-67  https://doi.org/10.1007/BF00034446
 
Hdider Chafik, Desjardins Yves (1994): Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets. Plant Cell, Tissue and Organ Culture, 36, 27-33  https://doi.org/10.1007/BF00048312
 
Janeiro LV, Vieitez AM, Ballester A (1995): Cold storage of in vitro cultures of wild cherry, chestnut and oak. Annales des Sciences Forestières, 52, 287-293  https://doi.org/10.1051/forest:19950309
 
Koch K. E. (1996): CARBOHYDRATE-MODULATED GENE EXPRESSION IN PLANTS. Annual Review of Plant Physiology and Plant Molecular Biology, 47, 509-540  https://doi.org/10.1146/annurev.arplant.47.1.509
 
Kovalchuk Irina, Lyudvikova Yelena, Volgina Mariam, Reed Barbara M. (2009): Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell, Tissue and Organ Culture (PCTOC), 96, 127-136  https://doi.org/10.1007/s11240-008-9468-8
 
Lambardi M., Roncasaglia R., Previati A., De Carlo A., Dradi G., Da Re F., Calamai L. (2006): In vitro slow growth storage of fruit rootstocks inside gas-tight or gas-permeable containers. Acta Horticulturae (ISHS), 725: 483–488.
 
Marino Grazia, Rosati Pasquale, Sagrati Fabrizio (1985): Storage of in vitro cultures of Prunus rootstocks. Plant Cell, Tissue and Organ Culture, 5, 73-78  https://doi.org/10.1007/BF00033572
 
Monette P.L. (1988): Grapevine (Vitiis vinifera L.). In: Bajaj Y.P.S. (ed.): Biotechnology in Agriculture and Forestry, 6: 3–37.
 
Moriguchi T., Kosaki S., Yamaki S., Sanada T. (1990): Low temperature storage of pear shoots in vitro. Journal Bulletin of Fruit Tree Research Station, 17: 11–18.
 
Mullin R.H., Schlegel D.E. (1976): Cold storage maintenance of strawberry meristem plantlets. HortScience, 11: 100–101.
 
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
 
Negri V., Tosti N., Standardi A. (2000): Slow growth storage of single node shoots of apple genotypes. Plant Cell Tissue Organ Culture, 62: 159–162. https://doi.org/10.1023/A:1026709706535
 
Orlikowska T. (1992): Effect of in vitro storage at 4°C on survival and proliferation of two apple rootstocks. Plant Cell Tissue Organ Culture, 31: 1–7.
 
Pérez-Tornero O., Ortín-Párraga F., Egea J., Burgos L. (1999): Medium-term storage of apricot shoot tips in vitro by minimal growth method. HortScience, 34: 1277–1278.
 
Pruski K., Kozai T., Lewis T., Astatkie T., Nowak J. (2000): Sucrose and light effects on in vitro cultures of potato, chokecherry and Saskatoon berry during low temperature storage. Plant Cell Tissue Organ Culture, 63: 215–221. https://doi.org/10.1023/A:1010649725527
 
R Core Team (2013): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at http://www.R-project.org/
 
Rao N.K. (2004): Plant genetic resources: Advancing conservation and use through biotechnology. African Journal of Biotechnology, 3: 136–145.
 
Reed B.M., Paynter C.L., DeNoma J., Chang Y.J. (1998): Techniques for medium and long-term storage of pear (Pyrus L.) genetic resources. Plant Genetic Resources Newsletter, 115: 1–5.
 
Romano A., Martins-Loução M.A. (1999): In vitro cold storage of cork oak shoot cultures. Plant Cell Tissue Organ Culture, 59: 155–157. https://doi.org/10.1023/A:1006465706905
 
Rybczyński Jan J., Borkowska Bożenna, Fiuk Agnieszka, Gawrońska Helena, Śliwińska Eleonora, Mikuła Anna (2007): Effect of sucrose concentration on photosynthetic activity of in vitro cultures Gentiana kurroo (Royle) germlings. Acta Physiologiae Plantarum, 29, 445-453  https://doi.org/10.1007/s11738-007-0054-1
 
Sauer A. (1985): In vitro propagation of Prunus avium L. and storage of in vitro derived plantlets. Acta Horticulturae (ISHS), 169: 351.
 
Ticha I., Cap F., Pacovska D., Hofman P., Haisel D., Capkova V., Schafer C. (1998): Culture on sugar medium enhances photosynthetic capacity and high light resistance of plantlets grown in vitro. Physiologia Plantarum, 102, 155-162  https://doi.org/10.1034/j.1399-3054.1998.1020201.x
 
Towill L.E. (1988): Genetic considerations for germplasm preservation of clonal materials. Horticultural Science, 23: 77–97.
 
Van Huylenbroeck Johan M., Debergh Pierre C. (1996): Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets. Physiologia Plantarum, 96, 298-304  https://doi.org/10.1034/j.1399-3054.1996.960220.x
 
Velerskov Bjarke (1987): Irradiance-dependent senescence of isolated leaves. Physiologia Plantarum, 71, 316-320  https://doi.org/10.1111/j.1399-3054.1987.tb04349.x
 
Weaver L. M., Amasino R. M. (2001): Senescence Is Induced in Individually Darkened Arabidopsis Leaves, but Inhibited in Whole Darkened Plants. PLANT PHYSIOLOGY, 127, 876-886  https://doi.org/10.1104/pp.010312
 
Wilkins C.P., Newbury H.J., Dodds J.H. (1988): Tissue culture conservation of fruit trees. Plant Genetic Resources Newsletter, 73/74: 9–20
 
Withers L.A. (1980): Tissue Culture Storage For Genetic Conservation. IBPGR Technical Report. Rome, FAO.
 
Wittenbach V. A. (1977): Induced Senescence of Intact Wheat Seedlings and Its Reversibility. PLANT PHYSIOLOGY, 59, 1039-1042  https://doi.org/10.1104/pp.59.6.1039
 
Yuanyuan M., Yali Z., Jiang L., Hongbo S. (2009): Roles of plant soluble sugars and their responses to plant cold stress. African Journal Biotechnology, 8: 2004–2010
 
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