Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potential
monitored in four-year-old regenerants

https://doi.org/10.17221/239/2017-HORTSCICitation:Pawłowska B., Neugebauerova J., Bieniasz M., Szewczyk-Taranek B., Ondrasek I. (2019): Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potentialmonitored in four-year-old regenerants. Hort. Sci. (Prague), 46: 90-97.
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Stability of Rosa agrestis, R. canina, R. dumalis and R. rubiginosa regenerants after shoot tip cryopreservation was monitored with the use of biochemical markers and pollen quality assessment in matured plants cultivated in the field. The results of rosa pollen and hip evaluation revealed no significant differences between post-cryopreserved regenerants and control plants; however, variations in the assessed markers were noted between Rosa species. Analyses of hips confirmed high levels of vitamin C in these plants; the highest was observed in R. dumalis (750–870 mg/100 g fresh weight). High total antioxidant activity (TAC) was as follows: DPPH – 50–250 mM TE/100 dry weight, FRAP – 80–180 mM TE/100 g dry weight. The present study showed a high content of flavonoids (TFC) and phenols (TPC). Post-cryopreserved plants retained pollen specific for Caninae: numerous anthers, abundant pollen, poor pollen germination and viability and typical pollen morphology. This study demonstrates new tools for evaluating the fidelity of post-cryopreserved regenerants.

References:
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BIENIASZ Monika, NECAS Tomas, DZIEDZIC Ewa, ONDRASEK Ivo, PAWŁOWSKA Bożena (2017): Evaluation of Pollen Quality and Self-Fertility in Selected Cultivars of Asian and European Pears. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45, 375- https://doi.org/10.15835/nbha45210877
 
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Ercisli Sezai (2008): Determination of pollen viability and <i>in vitro</i> pollen germination of <i>Rosa dumalis</i> and <i>Rosa villosa</i>. Bangladesh Journal of Botany, 36, 185-187 https://doi.org/10.3329/bjb.v36i2.1511
 
ERCISLI S (2007): Chemical composition of fruits in some rose (Rosa spp.) species. Food Chemistry, 104, 1379-1384 https://doi.org/10.1016/j.foodchem.2007.01.053
 
Fatemi N., Attar F., Assareh M.H., Hamzehee B. (2012): Pollen morphology of the genus Rosa L. (Rosaceae) in Iran. Iran Journal of Botany, 18: 284–293.
 
Harding K. (2004): Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25: 3–22.
 
Kaity Adam, Ashmore S. E., Drew R. A. (2009): Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones. Plant Cell Reports, 28, 1421-1430 https://doi.org/10.1007/s00299-009-0742-y
 
Kaity A., Drew R. A., Ashmore S. E. (2013): Genetic and epigenetic integrity assessment of acclimatised papaya plants regenerated directly from shoot-tips following short- and long-term cryopreservation. Plant Cell, Tissue and Organ Culture (PCTOC), 112, 75-86 https://doi.org/10.1007/s11240-012-0217-7
 
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Lachman J., Hamouz K., Čepl J., Pivec V., Šulc M., Dvořák P. (2006): Vliv vybraných faktoru na obsah polyfenolu a antioxidační aktivitu hlíz brambor. Chemické listy, 100: 522–527.
 
Martín Carmen, Kremer Carolina, González Iván, González-Benito María Elena (2015): Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 185-195 https://doi.org/10.1007/s11240-015-0760-0
 
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Pawłowska Bożena, Szewczyk-Taranek Bożena (2014): Droplet vitrification cryopreservation of Rosa canina and Rosa rubiginosa using shoot tips from in situ plants. Scientia Horticulturae, 168, 151-156 https://doi.org/10.1016/j.scienta.2013.12.016
 
Pawłowska Bożena, Szewczyk-Taranek Bożena (2015): Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants. Acta Societatis Botanicorum Poloniae, 84, 439-442 https://doi.org/10.5586/asbp.2015.038
 
Quave Cassandra L., Pieroni Andrea (2015): A reservoir of ethnobotanical knowledge informs resilient food security and health strategies in the Balkans. Nature Plants, 1, - https://doi.org/10.1038/nplants.2014.21
 
Roman Ioana, Stănilă Andreea, Stănilă Sorin (2013): Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal, 7, 73- https://doi.org/10.1186/1752-153X-7-73
 
Ružić Dj., Vujović T., Cerović R. (2014): Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. Horticultural Science, 41, 55-63 https://doi.org/10.17221/234/2013-HORTSCI
 
Shan Bin, Cai Yizhong Z., Sun Mei, Corke Harold (2005): Antioxidant Capacity of 26 Spice Extracts and Characterization of Their Phenolic Constituents. Journal of Agricultural and Food Chemistry, 53, 7749-7759 https://doi.org/10.1021/jf051513y
 
Sharma Neelam, Singh Rakesh, Pandey Ruchira, Kaushik Nutan (2017): Genetic and biochemical stability assessment of plants regenerated from cryopreserved shoot tips of a commercially valuable medicinal herb Bacopa monnieri (L.) Wettst. In Vitro Cellular & Developmental Biology - Plant, 53, 346-351 https://doi.org/10.1007/s11627-017-9826-5
 
Skyba M., Urbanowa M., Kapchina-Toteva V., Kosuth J., Harding K., Hellerova E. (2010): Physiological, biochemical and molecular characteristics of cryopreservated Hyperium perforatum L. shoot tips. CryoLetters, 31: 249–260.
 
STĂNILĂ Andreea, DIACONEASA Zoriţa, ROMAN Ioana, SIMA Nicușor, MĂNIUȚIU Dănuț, ROMAN Alin, SIMA Rodica (2015): Extraction and Characterization of Phenolic Compounds from Rose Hip (<i>Rosa canina</i> L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43, - https://doi.org/10.15835/nbha.43.2.10028
 
Taneva I., Petkova N., Dimov I., Ivan I., Denev P. (2016): Chracterization of rose hips (Rosa canina L.) fruits extracts and evaluation of their in vitro antioxidant activity. Journal of Pharmacognosy and Phytochemistry, 5: 35–38,
 
Wrońska-Pilarek D., Tomlik-Wyremblewska A. (2010): Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods. Dendrobiology, 64: 43–53.
 
Wrońska-Pilarek Dorota (2011): Pollen morphology of Polish native species of the Rosa genus (Rosaceae) and its relation to systematics. Acta Societatis Botanicorum Poloniae, 80, 221-232 https://doi.org/10.5586/asbp.2011.031
 
Yordanowa Z.P., Dimitrova M.A., Čellárová E., Kapchin-Toteva V.M. (2011): Physiological evaluation of Hypericum rumeliacum Boiss. Plants regenerated after cryopreservation. Cryopreservation Symposium, COST Action 871 “Cryopreservation of crop species in Europe”. Final meeting. Angers, France, February 8–11: 56–59
 
Zloch Z., Čelakovský J., Aujezdská A. (2004): Stanovení obsahu polyfenolu a celkové antioxidační kapacity v potravinách rostlinného původu. Závěrečná zpráva o plnění projektu podpořeného finančně Nadačním fondem Institutu Danone, Plzeň.
 
Zhang Zhibo, Skjeseth Gry, Elameen Abdelhameed, Haugslien Sissel, Sivertsen Astrid, Clarke Jihong Liu, Wang Qiao-Chun, Blystad Dag-Ragnar (2015): Field performance evaluation and genetic integrity assessment in Argyranthemum ‘Yellow Empire’ plants recovered from cryopreserved shoot tips. In Vitro Cellular & Developmental Biology - Plant, 51, 505-513 https://doi.org/10.1007/s11627-015-9707-8
 
Alexander M. P. (2009): Differential Staining of Aborted and Nonaborted Pollen. Stain Technology, 44, 117-122 https://doi.org/10.3109/10520296909063335
 
ALP Sevket, ERCISLI Sezai, JURIKOVA Tunde, CAKIR Ozlem, GOZLEKCI Sadiye (2016): Bioactive Content of Rose Hips of Different Wildly Grown <i>Rosa dumalis</i> Genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44, 472- https://doi.org/10.15835/nbha44210432
 
Arya S.P, Mahajan M, Jain P (2000): Non-spectrophotometric methods for the determination of Vitamin C. Analytica Chimica Acta, 417, 1-14 https://doi.org/10.1016/S0003-2670(00)00909-0
 
Bi W.L., Pan C., Liu J., Wang Q.C. (2016): Greenhouse performance, genetic stability and biochemical compounds in Chrysanthemum morifolium ‘Hangju’ plants regenerated form cryopreserved shoot tips. Acta Physiologia Plantarum, 38: 38–268.
 
BIENIASZ Monika, NECAS Tomas, DZIEDZIC Ewa, ONDRASEK Ivo, PAWŁOWSKA Bożena (2017): Evaluation of Pollen Quality and Self-Fertility in Selected Cultivars of Asian and European Pears. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45, 375- https://doi.org/10.15835/nbha45210877
 
Chrubasik Cosima, Roufogalis Basil D., Müller-Ladner Ulf, Chrubasik Sigrun (2008): A systematic review on theRosa canina effect and efficacy profiles. Phytotherapy Research, 22, 725-733 https://doi.org/10.1002/ptr.2400
 
Ercisli Sezai (2008): Determination of pollen viability and <i>in vitro</i> pollen germination of <i>Rosa dumalis</i> and <i>Rosa villosa</i>. Bangladesh Journal of Botany, 36, 185-187 https://doi.org/10.3329/bjb.v36i2.1511
 
ERCISLI S (2007): Chemical composition of fruits in some rose (Rosa spp.) species. Food Chemistry, 104, 1379-1384 https://doi.org/10.1016/j.foodchem.2007.01.053
 
Fatemi N., Attar F., Assareh M.H., Hamzehee B. (2012): Pollen morphology of the genus Rosa L. (Rosaceae) in Iran. Iran Journal of Botany, 18: 284–293.
 
Harding K. (2004): Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25: 3–22.
 
Kaity Adam, Ashmore S. E., Drew R. A. (2009): Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones. Plant Cell Reports, 28, 1421-1430 https://doi.org/10.1007/s00299-009-0742-y
 
Kaity A., Drew R. A., Ashmore S. E. (2013): Genetic and epigenetic integrity assessment of acclimatised papaya plants regenerated directly from shoot-tips following short- and long-term cryopreservation. Plant Cell, Tissue and Organ Culture (PCTOC), 112, 75-86 https://doi.org/10.1007/s11240-012-0217-7
 
Kulus D., Mikuła A. (2016): Cryopreservation in securing the stability and physiological activity of plant cells. Acta Scientiarum Polonorum Biotechnologia, 15: 27–38 (in Polish).
 
Lachman J., Hamouz K., Čepl J., Pivec V., Šulc M., Dvořák P. (2006): Vliv vybraných faktoru na obsah polyfenolu a antioxidační aktivitu hlíz brambor. Chemické listy, 100: 522–527.
 
Martín Carmen, Kremer Carolina, González Iván, González-Benito María Elena (2015): Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 185-195 https://doi.org/10.1007/s11240-015-0760-0
 
Nybom H., Werlemark G., Esselink D.G., Vosman B. (2005): SEXUAL PREFERENCES LINKED TO ROSE TAXONOMY AND CYTOLOGY. Acta Horticulturae, , 21-28 https://doi.org/10.17660/ActaHortic.2005.690.1
 
Nybom Hilde, Werlemark Gun (2016): Realizing the Potential of Health-Promoting Rosehips from Dogroses (Rosa sect. Caninae). Current Bioactive Compounds, 13, 3-17 https://doi.org/10.2174/1573407212666160607090635
 
Pawłowska Bożena, Szewczyk-Taranek Bożena (2014): Droplet vitrification cryopreservation of Rosa canina and Rosa rubiginosa using shoot tips from in situ plants. Scientia Horticulturae, 168, 151-156 https://doi.org/10.1016/j.scienta.2013.12.016
 
Pawłowska Bożena, Szewczyk-Taranek Bożena (2015): Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants. Acta Societatis Botanicorum Poloniae, 84, 439-442 https://doi.org/10.5586/asbp.2015.038
 
Quave Cassandra L., Pieroni Andrea (2015): A reservoir of ethnobotanical knowledge informs resilient food security and health strategies in the Balkans. Nature Plants, 1, - https://doi.org/10.1038/nplants.2014.21
 
Roman Ioana, Stănilă Andreea, Stănilă Sorin (2013): Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal, 7, 73- https://doi.org/10.1186/1752-153X-7-73
 
Ružić Dj., Vujović T., Cerović R. (2014): Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. Horticultural Science, 41, 55-63 https://doi.org/10.17221/234/2013-HORTSCI
 
Shan Bin, Cai Yizhong Z., Sun Mei, Corke Harold (2005): Antioxidant Capacity of 26 Spice Extracts and Characterization of Their Phenolic Constituents. Journal of Agricultural and Food Chemistry, 53, 7749-7759 https://doi.org/10.1021/jf051513y
 
Sharma Neelam, Singh Rakesh, Pandey Ruchira, Kaushik Nutan (2017): Genetic and biochemical stability assessment of plants regenerated from cryopreserved shoot tips of a commercially valuable medicinal herb Bacopa monnieri (L.) Wettst. In Vitro Cellular & Developmental Biology - Plant, 53, 346-351 https://doi.org/10.1007/s11627-017-9826-5
 
Skyba M., Urbanowa M., Kapchina-Toteva V., Kosuth J., Harding K., Hellerova E. (2010): Physiological, biochemical and molecular characteristics of cryopreservated Hyperium perforatum L. shoot tips. CryoLetters, 31: 249–260.
 
STĂNILĂ Andreea, DIACONEASA Zoriţa, ROMAN Ioana, SIMA Nicușor, MĂNIUȚIU Dănuț, ROMAN Alin, SIMA Rodica (2015): Extraction and Characterization of Phenolic Compounds from Rose Hip (<i>Rosa canina</i> L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43, - https://doi.org/10.15835/nbha.43.2.10028
 
Taneva I., Petkova N., Dimov I., Ivan I., Denev P. (2016): Chracterization of rose hips (Rosa canina L.) fruits extracts and evaluation of their in vitro antioxidant activity. Journal of Pharmacognosy and Phytochemistry, 5: 35–38,
 
Wrońska-Pilarek D., Tomlik-Wyremblewska A. (2010): Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods. Dendrobiology, 64: 43–53.
 
Wrońska-Pilarek Dorota (2011): Pollen morphology of Polish native species of the Rosa genus (Rosaceae) and its relation to systematics. Acta Societatis Botanicorum Poloniae, 80, 221-232 https://doi.org/10.5586/asbp.2011.031
 
Yordanowa Z.P., Dimitrova M.A., Čellárová E., Kapchin-Toteva V.M. (2011): Physiological evaluation of Hypericum rumeliacum Boiss. Plants regenerated after cryopreservation. Cryopreservation Symposium, COST Action 871 “Cryopreservation of crop species in Europe”. Final meeting. Angers, France, February 8–11: 56–59
 
Zloch Z., Čelakovský J., Aujezdská A. (2004): Stanovení obsahu polyfenolu a celkové antioxidační kapacity v potravinách rostlinného původu. Závěrečná zpráva o plnění projektu podpořeného finančně Nadačním fondem Institutu Danone, Plzeň.
 
Zhang Zhibo, Skjeseth Gry, Elameen Abdelhameed, Haugslien Sissel, Sivertsen Astrid, Clarke Jihong Liu, Wang Qiao-Chun, Blystad Dag-Ragnar (2015): Field performance evaluation and genetic integrity assessment in Argyranthemum ‘Yellow Empire’ plants recovered from cryopreserved shoot tips. In Vitro Cellular & Developmental Biology - Plant, 51, 505-513 https://doi.org/10.1007/s11627-015-9707-8
 
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