Molecular S-genotyping of sweet cherry (Prunus avium L.) genetic resources

https://doi.org/10.17221/245/2017-HORTSCICitation:Patzak J., Henychová A., Paprštein F., Sedlák J. (2019): Molecular S-genotyping of sweet cherry (Prunus avium L.) genetic resources. Hort. Sci. (Prague), 46: 146-152.
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Sweet cherries are self-incompatible, which is determined by a gametophytic self-incompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic S-locus. Knowledge about the S-allele constitution of the cultivars is essential for fruit growers and breeders. Recently, molecular PCR-based methods have been developed to distinguish all S-alleles in sweet cherries. In our work, we analysed S-locus genotypes by 13 universal and allele-specific PCR primer combinations within 117 registered, old and local sweet cherry cultivars from the Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy, the Czech Republic. We confirmed the previous S-genotyping for 66 accessions except for Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Badeborner, Bing, Alfa, Gamma, Huldra, Rivan, Valerij Tschkalov, Viola and Winkler’s Frühe. It could be due to either mislabelling or mistakes in the previous analyses. Newly, S-genotyping was determined for 51 accessions in which we found 4 new S-loci combinations. We detected the S-locus combinations in 19 incompatibility groups. The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6) with 22, 20, 12 and 12 genotypes, respectively.

 

References:
Cachi A., Wünsch A. (2014): S-genotyping of sweet cherry varieties from Spain and S-locus diversity in Europe. Euphytica, 197: 229–236. https://doi.org/10.1007/s10681-014-1061-0
 
De Cuyper D., Sonneveld T., Tobutt K.R. (2005): Determining self-incompatibility genotypes in Belgian wild cherries. Molecular Ecology, 14: 945–955. https://doi.org/10.1111/j.1365-294X.2005.02460.x
 
Dirlewanger E., Claverie J., Iezzoni A.F., Wünsch A. (2009): 14. Sweet and Sour Cherries: Linkage Maps, QTL Detection and Marker Assisted Selection. In: Folta K.M., Gardiner S.E. (eds.): Genetics and Genomics of Rosaceae, Plant Genetics and Genomics: Crops and Models 6, Springer Science+Business Media, LLC: 291–313.
 
Goulao L., Cabrita C.M., Oliviera C.M, Leitao J.M. (2001): Comparing RAPD and AFLP analysis in discrimination and estimation of genetic similarities among apple (Malus × domestica Borkh.) cultivars. Euphytica, 119: 259–270. https://doi.org/10.1023/A:1017519920447
 
Hanada T. et al. (2014): Two novel self-compatible S haplotypes in peach (Prunus persica). Journal of Japanese Society of Horticultural Science, 83: 203–213. https://doi.org/10.2503/jjshs1.CH-099
 
Iezzoni A.F. (2008): Cherries - Chapter 5. In: Hancock J.F. (ed): Temperate Fruit Crop Breeding: Germplasm to Genomics, New York, Springer: 150–175.
 
Lisek A., Rozpara E., Glowacka A., Kucharska D., Zawadska M. (2015): Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe. Horticultural Science, 42: 13–21. https://doi.org/10.17221/103/2014-HORTSCI
 
Patzak J. (2001): Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica, 121: 9––18.
 
Schuster M. (2012): Incompatible (S-) genotypes of sweet cherry cultivars (Prunus avium L.). Scientia Horticulurae, 148: 59–73. https://doi.org/10.1016/j.scienta.2012.09.012
 
Schuster M., Flachowsky H., Köhler D. (2007): Determination of self-incompatible genotypes in sweet cherry (Prunus avium L.) accessions and cultivars of the German Fruit Gene Bank and from private collections. Plant Breeding, 126: 533–540. https://doi.org/10.1111/j.1439-0523.2007.01401.x
 
Sharma K., Sedlák P., Zeka D., Vejl P., Soukup J. (2014): Allele-specific PCR detection of sweet cherry self-incompatibility alleles S3, S4 and S9 using consensus and allele-specific primers in the Czech Republic. Horticultural Science, 41: 153–159. https://doi.org/10.17221/89/2014-HORTSCI
 
Sonneveld T., Tobutt K.R., Robbins, T.P. (2003): Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers. Theoretical and Applied Genetics, 107: 1059–1070. https://doi.org/10.1007/s00122-003-1274-4
 
Tao R., Yamane H., Sugiura A., Murayama H., Sassa H., Mori H. (1999): Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry. Journal of the American Society for Horticultural Science, 124: 224–233. https://doi.org/10.21273/JASHS.124.3.224
 
Vaughan S.P., Boškovič R., Gisbert-Climent A., Russell K., Tobutt, K.R. (2008): Characterization of a novel S-alleles from cherry (Prunus avium L.). Tree Genetics and Genomes 4: 531–541. https://doi.org/10.1007/s11295-007-0129-6
 
Wiersma P.A., Wu Z., Zhou L., Hampson C., Kappel, F. (2001): Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis. Theoretical and Applied Genetics, 102: 700–708. https://doi.org/10.1007/s001220051700
 
Yamane H., Ikeda K., Ushijima K., Sassa H., Tao, R. (2003): A pollen-expressed gene for a novel protein with an F-box motif that is very tightly linked to a gene for S-RNase in two species of cherry, Prunus cerasus and P. avium. Plant and Cell Physiology, 44: 764–769. https://doi.org/10.1093/pcp/pcg088
 
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