In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) – Short Communication
E.J. Cheong, A.R. Jeon, J.W. Kang, R. Mock, G. Kinard, R. Lihttps://doi.org/10.17221/266/2013-HORTSCICitation:Cheong E.J., Jeon A.R., Kang J.W., Mock R., Kinard G., Li R. (2014): In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) – Short Communication. Hort. Sci. (Prague), 41: 95-99.
Black raspberry necrosis virus (BRNV) is one of the most important viral pathogens of Rubus spp. In this study, a procedure combining in vitro culture and heat therapy on axillary buds of Rubusoccidentalis was developed to eliminate BRNV from infected plants. Axillary buds were grown aseptically at 4-h alternating periods of 29°C and 38°C with 14-h day length for 1–5 weeks. Shoots induced after thermotherapy were tested for the viruses by RT-PCR. BRNV was not detected in any shoots indicating that BRNV was heat labile. BRNV-free shoots were rooted in vitro and acclimatized in greenhouse. Regenerated plants remained virus-free after 2-month acclimatization, 3-month dormancy and 5-month growth periods.Keywords:
BRNV; Rubus occidentalis; axillary bud culture; thermotherapy