Determination of antioxidant activities of some apple cultivars L., Ünüvar G., Ersoy N. (2017): Determination of antioxidant activities of some apple cultivars . Hort. Sci. (Prague), 44: 120-125.
download PDF

This study was conducted in order to determine the antioxidant activities of certain apple varieties. ‘Galaxy Gala’, ‘Scarlet Spur’, ‘Fuji’, ‘Pink Lady’ and ‘Granny Smith’ were characterized in this study. The antioxidant activities among the varieties were determined via three different procedures. Antiradical activity figures of the apple varieties were between 0.592 (‘Galaxy Gala’) and 0.802 (‘Scarlet Spur’). Inhibition levels of chelating activity with Fe+2 varied between 40.20–55.50%; ‘Scarlet Spur’ and ‘Pink Lady’ extracts had the highest inhibition with 55.50% and ‘Granny Smith’ extract had the lowest with the level. Among the extracts showing H2O2 clearance effect, Granny Smith (62.54%) and Scarlet Spur (44.67%) extracts had the highest inhibition levels. In this study conducted on apple varieties, total phenolic matter levels were determined in ‘Scarlet Spur’, ‘Pink Lady’, ‘Fuji’, ‘Galaxy Gala’ and ‘Granny Smith’.

Altun M.L., Çıtoğlu G.S., Yılmaz B.S., Çoban T. (2007): Antioxidant properties of Viburnum opulus and Viburnum lantana growing in Turkey. International Journal of Food Science and Nutrition, 5: 1–6.
Anonymous (2013): The R Project for Statistical Computing. Available at
Block Gladys, Patterson Blossom, Subar Amy (1992): Fruit, vegetables, and cancer prevention: A review of the epidemiological evidence. Nutrition and Cancer, 18, 1-29
Boyer Jeanelle, Liu Rui Hai (2004): Apple phytochemicals and their health benefits. Nutrition Journal, 3, -
Brand-Williams W., Cuvelier M.E., Berset C. (1995): Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28, 25-30
Couladis Maria, Tzakou Olga, Verykokidou Evmorfia, Harvala Catherine (2003): Screening of some Greek aromatic plants for antioxidant activity. Phytotherapy Research, 17, 194-195
Çoban Tülay, Çitoǧlu Gülçin Saltan, Sever Betül, İşcan Mümtaz (2008): Antioxidant Activities of Plants Used in Traditional Medicine in Turkey. Pharmaceutical Biology, 41, 608-613
da Silva Porto Patrı́cia Andréia Leite, Laranjinha João António Nave, de Freitas Victor Armando Pereira (2003): Antioxidant protection of low density lipoprotein by procyanidins: structure/activity relationships. Biochemical Pharmacology, 66, 947-954
Drogoudi Pavlina D., Michailidis Zisis, Pantelidis George (2008): Peel and flesh antioxidant content and harvest quality characteristics of seven apple cultivars. Scientia Horticulturae, 115, 149-153
Escarpa A, González M.C (1998): High-performance liquid chromatography with diode-array detection for the determination of phenolic compounds in peel and pulp from different apple varieties. Journal of Chromatography A, 823, 331-337
Eskin N.A.M., Henderson H.M., Townsend R.J. (1976): Biochemie der Lebensmittel. Hüthig Verlag.
Griffits H.R., Lunec J., Arouma O.I., Halliwell B. (1998): Molecular Biology of Free Radicals in Human Diseases. London, Oica International.
Halliwell B. (2001): Food-derived antioxidants: How to evaluate their importance in food and in vivo. Handbook of Antioxidants. Los Angeles, New York, CRC Press.
Imeh U., Khokhar S. (2002): Distribution of Conjugated and Free Phenols in Fruits:  Antioxidant Activity and Cultivar Variations. Journal of Agricultural and Food Chemistry, 50, 6301-6306
Karaman Ş. (2008): Türkiye’de yetiştirilen bazı elma çeşitlerinin toplam antioksidan kapasitelerinin ve antioksidan özellik gösteren başlıca bileşenlerinin karşılaştırılması. [MSc Thesis.] İstanbul Üniversitesi Fen Bilimleri Enstitüsü Kimya Anabilim Dalı, İstanbul, 116–127.
Korban S.S., Skirvin R.M. (1984): Nomenclature of the Cultivated Apple. HortScience, 19: 177–180.
Lee Ki Won, Kim Young Jun, Kim Dae-Ok, Lee Hyong Joo, Lee Chang Yong (2003): Major Phenolics in Apple and Their Contribution to the Total Antioxidant Capacity. Journal of Agricultural and Food Chemistry, 51, 6516-6520
Leontowicz Hanna, Gorinstein Shela, Lojek Antonin, Leontowicz Maria, Čı́ž Milan, Soliva-Fortuny Robert, Park Yong-Seo, Jung Soon-Teck, Trakhtenberg Simon, Martin-Belloso Olga (2002): Comparative content of some bioactive compounds in apples, peaches and pears and their influence on lipids and antioxidant capacity in rats. The Journal of Nutritional Biochemistry, 13, 603-610
Liu R.H. (2003): Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. American Journal of Clinical Nutrition, 78(3 Suppl): 517S–520S.
Miller Nicholas J., Rice-Evans Catherine A. (1997): The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Food Chemistry, 60, 331-337
Molyneux P. (2004): The use of the stable free radical diphenylpicryl-hydrazl (DPPH) for estimating antioxidant activity. Songklanakarn Journal of Science and Technology, 26: 211–219.
OH HOON-IL, HOFF JOHAN E. (1987): pH Dependence of Complex Formation Between Condensed Tannins and Proteins. Journal of Food Science, 52, 1267-1269
Rice-Evans Catherine, Miller Nicholas, Paganga George (1997): Antioxidant properties of phenolic compounds. Trends in Plant Science, 2, 152-159
Rival Sandrine G., Boeriu Carmen G., Wichers Harry J. (2001): Caseins and Casein Hydrolysates. 2. Antioxidative Properties and Relevance to Lipoxygenase Inhibition. Journal of Agricultural and Food Chemistry, 49, 295-302
Ruch Randall J., Cheng Shu-jun, Klaunig James E. (1989): Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, 1003-1008
HANNUM SANDRA M. (2004): Potential Impact of Strawberries on Human Health: A Review of the Science. Critical Reviews in Food Science and Nutrition, 44, 1-17
Singleton V.L., Rossi J.R. (1965): Colorimetry of total phenolics with phosphomolibdic-phosphothungstic acid. American Journal of Enology Viticulture, 16: 144–158.
Stadtman Earl R (2002): Importance of individuality in oxidative stress and aging 1,2 1Guest Editor: Rajindar S. Sohal 2This article is part of a series of reviews on “Oxidative Stress and Aging.” The full list of papers may be found on the homepage of the journal.. Free Radical Biology and Medicine, 33, 597-604
Sun Jie, Chu Yi-Fang, Wu Xianzhong, Liu Rui Hai (2002): Antioxidant and Antiproliferative Activities of Common Fruits. Journal of Agricultural and Food Chemistry, 50, 7449-7454
Tepe Bektas, Sokmen Munevver, Akpulat H. Askin, Sokmen Atalay (2006): Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chemistry, 95, 200-204
Vinson Joe A, Zubik Ligia, Bose Pratima, Samman Najwa, Proch John (2005): Dried Fruits: Excellent in Vitro and in Vivo Antioxidants. Journal of the American College of Nutrition, 24, 44-50
Yen G (): Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae. Food Chemistry, 65, 375-379
download PDF

© 2020 Czech Academy of Agricultural Sciences