Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane- 1-carboxylate oxidase 1 (ACO1) gene in apple – Short Communication

https://doi.org/10.17221/326/2014-HORTSCICitation:Marić S. (2016): Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane- 1-carboxylate oxidase 1 (ACO1) gene in apple – Short Communication. Hort. Sci. (Prague), 43: 100-104.
download PDF
Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This paper presents the results of molecular cloning and characterization of a newly identified allele of the ripening-specific ACO1 gene from Malus floribunda 821. A polymorphism of the ACO1 gene was detected using the polymerase chain reaction and BamH1 and RsaI enzymatic digestion. Whilst cloning and sequencing data confirmed the identity of allele d, it also revealed high sequence conservation (97.3 to 98.9%). In addition, a comparison of sequence data revealed some differences within the coding regions of the ACO1 alleles identified so far.
References:
Abeles F.B., Morgan P.W., Saltveit, Jr M.E. (1992): Ethylene in plant biology. San Diego, Academic Press, Inc.
 
Binnie Jan E., McManus Michael T. (2009): Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh. Phytochemistry, 70, 348-360  https://doi.org/10.1016/j.phytochem.2009.01.002
 
Castiglione S., Pirola B., Sala F., Ventura M., Pancaldi M., Sansavini S. (1999): Molecular studies of ACC synthase and ACC oxidase genes in apple. Acta Horticulturae (ISHS), 484: 305‒309.
 
Costa Fabrizio, Stella Sara, Weg W. Eric, Guerra Walter, Cecchinel Michela, Dallavia Joseph, Koller Bernie, Sansavini Silviero (2005): Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh). Euphytica, 141, 181-190  https://doi.org/10.1007/s10681-005-6805-4
 
Doyle J.J., Doyle L.J. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11‒15.
 
Marić Slađana, Lukić Milan, Flachowsky H. (2014): Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple ( Malus  ×  domestica Borkh.). Plant Breeding, 133, 108-114  https://doi.org/10.1111/pbr.12124
 
Marić S., Bošković R., Tešović Ž., Lukić M. (2005): Genetical polymorphism of ACC synthase and ACC oxidase in apple selections bred in Čačak, Genetika, 37: 225–235.
 
Wakasa Y., Kudo H., Ishikawa R., Akada S., Senda M., Niizeki M., Harada T. (2006): Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential. Postharvest Biology and Technology, 39, 193-198  https://doi.org/10.1016/j.postharvbio.2005.10.005
 
Yang S.F. (1985): Biosynthesis and action of ethylene. HortScience, 20: 41‒45.
 
Yoo Ahrim, Seo Young Sam, Jung Jin-Won, Sung Soon-Kee, Kim Woo Taek, Lee Weontae, Yang Dae Ryook (2006): Lys296 and Arg299 residues in the C-terminus of MD-ACO1 are essential for a 1-aminocyclopropane-1-carboxylate oxidase enzyme activity. Journal of Structural Biology, 156, 407-420  https://doi.org/10.1016/j.jsb.2006.08.012
 
download PDF

© 2020 Czech Academy of Agricultural Sciences