Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seeds

https://doi.org/10.17221/351/2014-HORTSCICitation:Jeung Keun S., Ji Hee K., Ae Kyung L. (2016): Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seeds. Hort. Sci. (Prague), 43: 84-91.
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Seed germination experiments with Corylopsis coreana, C. sinensis var. calvescens, and Corylopsis gotoana were conducted.  Corylopsis coreana seeds were treated at 5o, 7.5o, 10o, 12.5o and 20oC to study the upper temperature limit to break dormancy.  Seeds of C. sinensis var. calvescens were treated 1 mo of warm stratification (WS) at 10, 15, 20, and 25oC followed by CS at 5oC for 0, 1, 2, and 3 mo to understand the requirement of WS and CS to effectively break dormancy.  Germination of Corylopsis seeds at 15o-20oC without CS suggests that dormancy is considered shallow and the upper temperature limit to break is 12.5oC.  Immersing seeds in water or ethanol induced seed germination which may result from leaching of inhibitors from the seed.  Observation of seed coats following treatment with water and ethanol by means of low temperature scanning electron microscopy (LT-SEM) revealed elongated and rectangular/hexagonal shaped cells in C. coreana and irregular jigsaw puzzle-piece shapes in C. gotoana.  In conclusion, dormancy in Corylopsis is considered shallow and the optimum CS is suggested to be 7.5oC, while optimum WS is ranging from12.5o to 20oC.
References:
Ali-Rachedi Sonia, Bouinot Denise, Wagner Marie-H�l�ne, Bonnet Magda, Sotta Bruno, Grappin Philippe, Jullien Marc (2004): Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta, 219, -  https://doi.org/10.1007/s00425-004-1251-4
 
Barbary O.M., Al-sohaimy S.A., El-Saadani M.A., Zeitoun A.M.A. (2009): Extraction, composition and physicochemical properties of flaxseed mucilage. Journal of The Advanced Agriculture Researches, 14: 605–622.
 
Baskin J.M., Baskin C.C (2004): A classification system for seed dormancy. Seed Science Research, 14: 1–16.
 
Baskin C. C., Zackrisson O., Baskin J. M. (2002): Role of warm stratification in promoting germination of seeds of Empetrum hermaphroditum (Empetraceae), a circumboreal species with a stony endocarp. American Journal of Botany, 89, 486-493  https://doi.org/10.3732/ajb.89.3.486
 
Batlla D.* , Benech-Arnold R.L. (): A quantitative analysis of dormancy loss dynamics in <I>Polygonum aviculare</I> L. seeds: Development of a thermal time model based on changes in seed population thermal parameters. Seed Science Research, 13, 55-68  https://doi.org/10.1079/SSR2002124
 
Cavanagh A.K. (1980): Some aspects of the history of seed coat treatments applied to acacias. International Group for the Study of Mimosoideae. Bulletin, 8: 31–36.
 
Dirr M. (1990): Manual of woody landscape plants: Their identification, ornamental characteristics, culture, propagation and uses. Champaign, Stipes Publishing Co. Haughn.
 
Haughn George, Chaudhury Abed (2005): Genetic analysis of seed coat development in Arabidopsis. Trends in Plant Science, 10, 472-477  https://doi.org/10.1016/j.tplants.2005.08.005
 
Hilhost H.W.M. (2008): Definition and hypothesis of seed dormancy. In: Bradford K., Nonogaki H. (eds): Seed Development, Dormancy and Germination. Oxford, Blackwell Publishing: 50–71.
 
Horimoto Taiga, Koshioka Masaji, Kubota Satoshi, Mander Lewis N., Hirai Nobuhiro, Ishida Nobuaki, Suh Jeung Keun, Lee Ae Kyung, Roh Mark S. (2011): Effect of warm and cold stratification on 1H-NMR profiles, endogenous gibberellins and abscisic acid in Styrax japonicus seeds. Horticulture, Environment, and Biotechnology, 52, 233-239  https://doi.org/10.1007/s13580-011-0207-4
 
Kwon H.J. (1995): Ecological characteristics, propagation method, and new cultivar of Korean Styrax japonicus Sieb. et Zucc. for landscape tree uses. [Ph.D. Thesis.] Sung Kyun Kwan Univeristy, Suwon, Korea (in Korean).
 
X. Liu Sean (2012): Extraction and Characterization of Proanthocyanidins from Grape Seeds. The Open Food Science Journal, 6, 5-11  https://doi.org/10.2174/1874256401206010005
 
(2002): Micropropagation of Juvenile and Mature Tree of Corylopsis coreana by Axillary Bud Culture. Journal of Plant Biotechnology, 29, 117-121  https://doi.org/10.5010/JPB.2002.29.2.117
 
Pipinis E., Milios E., Aslanidou M., Mavrokordo O., Smiris P. (2009): The Effect of Stratification on Seed Germination of Jasminus fruticans L. (Oleaceae): A Contribution to a Better Insight on the Species Germination Ecology. International Journal of Botany, 5, 181-185  https://doi.org/10.3923/ijb.2009.181.185
 
Rehman S, Park In-Hwan (2000): Effect of scarification, GA and chilling on the germination of goldenrain-tree (Koelreuteria paniculata Laxm.) seeds. Scientia Horticulturae, 85, 319-324  https://doi.org/10.1016/S0304-4238(00)00126-6
 
Roh Mark, Bentz Jo-Ann, Wang Paul, Li Ercheng, Koshioka Masaji (2015): Maturity and temperature stratification affect the germination of Styrax japonicus seeds. The Journal of Horticultural Science and Biotechnology, 79, 645-651  https://doi.org/10.1080/14620316.2004.11511820
 
Roh Mark S., Lee Ae-Kyung, Suh Jeung Keun, Bordelon Carole M. (2008): Interspecific variations in seed germination of Corylopsis. Scientia Horticulturae, 118, 347-350  https://doi.org/10.1016/j.scienta.2008.06.026
 
Siddiqui Z.S., Khan M.A. (2010): The role of seed coat phenolics on water uptake and early protein synthesis during germination of dimorphic seeds of Halopyrum mucronatum (L.) Spath. Pakistan Journal of Botany, 42: 227–238.
 
Suzuki T., Matsuura T., Kawakami N., Noda K. (2000): Accumulation and leakage of abscisic acid during embryo development and seed dormancy in wheat. Plant Growth Regulation, 30: 253–260.  https://doi.org/10.1023/A:1006320614530
 
Western Tamara L. (2012): The sticky tale of seed coat mucilages: production, genetics, and role in seed germination and dispersal. Seed Science Research, 22, 1-25  https://doi.org/10.1017/S0960258511000249
 
Taylorson R. B., Hendricks S. B. (1979): Overcoming dormancy in seeds with ethanol and other anesthetics. Planta, 145, 507-510  https://doi.org/10.1007/BF00380106
 
Wada Sugae, Kennedy James A., Reed Barbara M. (2011): Seed-coat anatomy and proanthocyanidins contribute to the dormancy of Rubus seed. Scientia Horticulturae, 130, 762-768  https://doi.org/10.1016/j.scienta.2011.08.034
 
Young J.A., Young C.G. (1992): Seeds of woody plants in North America. Portalnd, Dioscorides Press.
 
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