Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting
V. Sarropoulou, K. Dimassi-Theriou, I. Therioshttps://doi.org/10.17221/359/2014-HORTSCICitation:Sarropoulou V., Dimassi-Theriou K., Therios I. (2015): Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting . Hort. Sci. (Prague), 42: 185-192.
The effects of two strength media (full and half) in inorganics and five polyvinylpyrrolidone (PVP) concentrations combined with 1 mg/l indole-3-butyric acid on the in vitro rooting of the CAB-6P, Gisela 6 and M × M 14 cherry rootstocks were investigated. For the CAB-6P, root number (5.80) was greatest by adding 5 g/l PVP to the half strength medium and 1 g/l PVP resulted in 80% rooting percentage. For Gisela 6, 1 g/l PVP in half strength medium led to the highest root number (6.30) as well as the highest rooting percentage (90.91%). On the contrary, root length was maximum (36.17 mm) in the full strength medium without adding PVP. In M × M 14, root number was maximum (8.33) in the PVP-free full strength medium, rooting percentage was 100% by adding 5 g/l PVP to either full or half strength medium and root length was greatest by incorporating 1 or 2.5 g/l PVP into the half strength medium. PVP seems to be a promising rooting agent in tissue culture systems.Keywords:
antioxidants; auxins; callus formation; plant tissue culture; rhizogenesisReferences:
Aka-Kacar Y., Akpinar C., Agar A., Yalcin-Mendi Y., Serce S., Ortas I. (2010): The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstocks during acclimatization. Romanian Biotechnological Letters, 15: 5246–5252.AMZALLAG G. N., LERNER H. R., POLJAKOFF-MAYBER A. (1992): Interaction Between Mineral Nutrients, Cytokinin and Gibberellic Acid During Growth of Sorghum at High NaCI Salinity. Journal of Experimental Botany, 43, 81-87 https://doi.org/10.1093/jxb/43.1.81Andersen R.L., Robinson T., Lang G.A. (1999): Managing the Gisela cherry rootstocks. New York Fruit Quarterly, 7: 1–4.Araújo J.M.A. (2008): Food Chemistry: Theory and Practice. 4th Ed. Universidade Federal de Viçosa, Minas Gerais, Viçosa: 596.Chang S.H., Yang J.C. (1996): Enhancement of plant formation from embryo cultures of Taxus mairei using suitable culture medium and PVP. Botanical Bulletin Academia Sinica, 37: 35–40.Cheng Benxu, Peterson Curt M., Mitchell Robert J. (1992): The role of sucrose, auxin and explant source on in vitro rooting of seedling explants of Eucalyptus sideroxylon. Plant Science, 87, 207-214 https://doi.org/10.1016/0168-9452(92)90152-CMelo Lucas Amaral de, Xavier Aloisio, Takahashi Elizabete Keiko, Rosado Antônio Marcos, Paiva Haroldo Nogueira de (2011): Effectiveness of ascorbic acid and PVP in the rooting of clonal mini-cuttings of Eucalyptus urophylla x Eucalyptus grandis. CERNE, 17, 499-507 https://doi.org/10.1590/S0104-77602011000400008Dimassi-Theriou K. (1995): In vitro rooting of rootstock GF 677 (P. persica × P. amygdalus) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70: 105–108.Dimassi-Theriou K., Economou A. (1993): The effect of the explant type and the nutrient substrate composition on shoot production in in vitro cultures of the rootstock GF-677 (P. persica × P. amygdalus). In: Scientific Annals Thessaloniki Aristotle University of Thessaloniki: 31–35.Dimassi-Theriou K., Therios I. (2006): General Pomology – Part A: Propagation and Rootstocks of Fruit Trees. Thessaloniki, Gartaganis.Driver J.A., Suttle G.R. (1987): Nursery handling of propagles. In: Bonga J.M., Durzan D.J. (eds): Cell and Tissue Culture in Forestry. Dordrecht, Springer: 320–335.Edin M., Garcin A., Lichou J., Jourdain J.M. (1996): Influence of dwarfing cherry rootstocks on fruit production. Proceedings of International Cherry Symposium. Acta Hortuculturae (ISHS), 410: 507–510.Fachinello J.C., Hoffmann A., Menezes A.L., Nachtigal J.C. (1993): Effect of PVP and IBA on rooting of strawberry guava (Psidium cattleyanum Sabine) on different substrates. Revista Brasileira de Fisiologia Vegetal, São Carlos, 5.Fotopoulos S., Sotiropoulos T.E. (2005): In vitro rooting of PR 204/84 rootstock (Prunus persica × P. amygdalus) as influenced by mineral concentration of the culture medium and exposure to darkness for a period. Agronomy Research, 3: 3–8.Fouad M.M., Gomaa A.H., El-Zaher M.H. (1997): Factors influencing in vitro establishment and multiplication stages of peach. Horticultural Abstracts, 67: 69.Ganesan M., Jayabalan N. (2006): Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian Journal of Experimental Biology, 44: 506–513.Hrotkó K., Magyar L., Simon G., Hanusz B. (1997): EFFECT OF ROOTSTOCKS AND INTERSTOCKS ON GROWTH AND YIELD OF SWEET CHERRY TREES. Acta Horticulturae, , 231-236 https://doi.org/10.17660/ActaHortic.1997.451.24Ibrahim A.I. (1999): Somaclonal variation during micropropagation of date palm via embryogenesis. The first International Conference in Egypt on Plant Tissue Culture and its Application: 189–199.Kappel F. (1993): Sweet cherry management in Europe. Compact Fruit Tree, 26: 123–124.Kappel F., Lichou J. (1994): Flowering and fruiting of Burlat sweet cherry on size-controlling rootstock. HortScience, 29: 611–612.Lis-Balchin M. (1989): The use of antioxidants as rooting enhancers in the Geraniaceae. Journal of Horticultural Science and Biotechnology, 64: 617–623.Long L.E., Kaiser C. (2010): Sweet cherry rootstocks for the Pacific Northwest. A Pacific Northwest Extension Publication Oregon State University, University of Idaho, Washington State University.Malá J., Kálal J., Cvrcková H., Cvikrová M., Eder J. (1999): The effect of reduction of exuded phenolic substances level on rooting of oak microcuttings. Acta Horticulturae (ISHS): 530: 353–362.Melo B., Pinto J.e.b.p., Luz J.m.q., Peixoto J.R., Juliatti F.C. (2001): Different antioxidants in oxidation control, germination and seedling development in vitro culture of embryos guarirobeira (Syagrus oleracea (Mart.) Becc.). Ciência e Agrotecnologia, 25: 1301–1306.Mohan Jain S., Häggman H. (2007): Protocols for Micropropagation of Woody Trees and Fruits. The Netherlands, Springer-Verlag: 559.Mollel M.H.N., Goyvaerts E.M.A. (2012): Micropropagation of marula, Sclerocarya birrea subsp. caffra (Anarcadiaceae) by axillary bud proliferation and random amplified polymorphic DNA (RAPD) analysis of plantlets. African Journal of Biotechnology, 11: 16003–16012.Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.xOrlikowska T. (1992): Effects of mineral composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocksin vitro. Biologia Plantarum, 34, 45-52 https://doi.org/10.1007/BF02925789Parris J.K. (2012): Basal salt composition, cytokinins, and phenolic binding agents influence in vitro growth and ex vitro establishment of Magnolia ‘Ann’. Hortscience, 47: 1625–1629.Qu R., Chaudhury A. (2001): Improved young inflorescence culture and regeneration of ‘Tifway’ Bermudagrass (Cynodon transvaalensis × C. dactylon). International Turfgrass Society Research Journal, 9: 198–201.Reustle G., Natter I. (1994): Effect of polyvinylpyrrolidone and activated charcoal on formation of microcallus from grapevine protoplasts (Vitis sp.). Vitis, 33: 117–121.Ruzic D., Rosati P., Marino G. (1984): Effect of plant growth regulators on micropropagation of peach and almond hybrid GF 677. Rivista di ortoflorofrutticoltura italiana, 68: 413–422.Standardi A., Romani F. (1990): Effects of some antioxidants on in vitro rooting of apple shoots. HortScience, 25: 1435–1436.Tang Wei, Harris LatoyaC., Outhavong Vilay, Newton RonaldJ. (2004): Antioxidants enhance in vitro plant regeneration by inhibiting the accumulation of peroxidase in Virginia pine (Pinus virginiana Mill.). Plant Cell Reports, 22, - https://doi.org/10.1007/s00299-004-0781-3Teixeira J.B. (2001): Limitações ao processo de cultivo in vitro de espécies lenhosas. Brasília: Embrapa – Recursos Genéticos e Biotecnologia, 2001. Available at http://www.redbio.org/portal/encuentros/enc_2001/index.htmVAARIO Lu-Min, OTOMO Yasuhiro, SODA Ryo, IDE Yuji (1995): Plant Regeneration from Root Tissue and Establishment of Root Culture of Japanese White Birch (Betula platyphylla var. japonica).. Plant tissue culture letters, 12, 251-258 https://doi.org/10.5511/plantbiotechnology1984.12.251Wendling I. (2002): Rejuvenation of Eucalyptus grandis by serial minicutting and micropropagation. [Ph.D. Thesis.] Federal University of Viçosa, Viçosa, Brasil.