Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones
I. Sowik, M. Markiewicz, L. Michalczukhttps://doi.org/10.17221/360/2014-HORTSCICitation:Sowik I., Markiewicz M., Michalczuk L. (2015): Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones. Hort. Sci. (Prague), 42: 141-148.
Somaclonal variation is a novel source of useful traits in strawberry breeding. In our previous work we selected K40 clone from in vitro cultures of strawberry cv. Elsanta, which was characterized by high resistance to Verticillium dahliae. The objective of this work was to evaluate the stability and transmission of this trait during in vitro shoot proliferation and traditional clonal propagation from runners. K40, Elsanta and Senga Sengana plants were propagated in vitro for 45 generations/passages or for 4 generation of clonal propagation from runners. The resistance/susceptibility of such derived plants to V. dahliae was tested in controlled conditions in a greenhouse. Irrespective of the method of propagation, the plants of K40 clone were much more resistant to V. dahliae than the plants of the original cultivar and the plants of ‘Senga Sengana’, which is a cultivar considered as resistant to Verticillium wilt in field conditions. This proves that this trait elicited at somaclonal variant is stable and transferable both during in vitro micropropagation and clonal plant propagation from runners.Keywords:
somaclonal variation; in vitro selection; plant pathogen; Verticillium wiltReferences:
Amil-Ruiz F., Blanco-Portales R., Munoz-Blanco J., Caballero J. L. (): The Strawberry Plant Defense Mechanism: A Molecular Review. Plant and Cell Physiology, 52, 1873-1903 https://doi.org/10.1093/pcp/pcr136Biswas M.K., Dutt M., Roy U.K., Islam R., Hossain M. (2009): Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Scientia Horticulturae, 122, 409-416 https://doi.org/10.1016/j.scienta.2009.06.002Boxus P. (1974): The production of strawberry plants by in vitro micropropagation. Journal of Horticultural Science, 49: 209–210.Battistini C., Rosati P. (1991): In vitro evaluation of somaclonal strawberry (Fragaria × ananassa ‘Brighton’) variants for susceptibility to Phytophthora cactorum. In: Dale A., Lubby W.W. (eds): The Strawberry into the 21st Century. Portland, Timber Press: 121–123.Cameron J.S., Hancock J.F. (1986): Enhanced growth vigour in vegetative progeny of micropropagated strawberry plants. HortScience, 21: 1225–1226.Karp Angela (1995): Somaclonal variation as a tool for crop improvement. Euphytica, 85, 295-302 https://doi.org/10.1007/BF00023959FAOSTAT (2012): FAO Statistical Databases. Available at http://faostat3.fao.orgForni Cinzia, Brandizzi Federica, Frattarelli Andrea, Damiano Carmine (): Comparative analysis of DNA nuclear content by flow cytometry on strawberry plants propagated via runners and regenerated from meristem and callus cultures. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 135, 169-174 https://doi.org/10.1080/11263500112331350790FRADIN EMILIE F., THOMMA BART P. H. J. (2006): Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Molecular Plant Pathology, 7, 71-86 https://doi.org/10.1111/j.1364-3703.2006.00323.xFukuoka H., Murata T., Komura A., Nakao S., Shimamura T., Nakao E., Kinouchi H. (1996): A new strawberry cultivar ‘Himatsuri’. Proceedings of School of Agriculture, Kyushu Tokai University, 15: 19–25.Goicoechea N. (2009): To what extent are soil amendments useful to control Verticillium wilt? Pest Management Science, 65: 831–839.Hammerschlag Freddi, Garcés Sandra, Koch-Dean Margery, Ray Stephanie, Lewers Kim, Maas John, Smith Barbara J. (2006): In vitro response of strawberry cultivars and regenerants to Colletotrichum acutatum. Plant Cell, Tissue and Organ Culture, 84, 255-261 https://doi.org/10.1007/s11240-005-9027-5Jain S.M. (2001): Tissue-derived variation in crop improvement. Euphytica, 118: 153–166. https://doi.org/10.1023/A:1004124519479Kaeppler S.M., Kaeppler H.F., Rhee S. (2000): Epigenetic aspects of somaclonal variation in plants. Plant Molecular Biology, 43: 179–188. https://doi.org/10.1023/A:1006423110134Knop W. (1965): Quantitative Untersuchungen über die Ernahrungsprozesse der Pflanzen. Die Landwirthschaftlichen Versuchs-Stationen, 7: 93–107.López-Escudero Francisco Javier, Mercado-Blanco Jesús (2011): Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen. Plant and Soil, 344, 1-50 https://doi.org/10.1007/s11104-010-0629-2McKinney H.H. (1923): A new system of grading plant diseases. Journal of Agricultural Research, 26: 195–218.Meszka B., Bielenin A. (2009): Bioproducts in control of strawberry Verticillium wilt. Phytopathologia, 52: 21–27.Miguel C., Marum L. (): An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. Journal of Experimental Botany, 62, 3713-3725 https://doi.org/10.1093/jxb/err155Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.xNallanchakravarthula Srivathsa, Mahmood Shahid, Alström Sadhna, Finlay Roger D., McCluskey Kevin (2014): Influence of Soil Type, Cultivar and Verticillium dahliae on the Structure of the Root and Rhizosphere Soil Fungal Microbiome of Strawberry. PLoS ONE, 9, e111455- https://doi.org/10.1371/journal.pone.0111455Peschke V.M., Phillips R.L. (1992): Genetic implications of somaclonal variation in plants. Advances in Genetics, 30: 41–75.Popescu A.N., Isac V.S., Coman M.S., Radulescu M.S. (1997): Somaclonal variation in plants regenerated by organogenesis from callus cultures of strawberry (Fragaria × ananassa).Acta Horticulturae (ISHS), 439: 89–96.Sansavini S., Rosati P., Gaggioli D., Toshi M.F. (1990): Inheritance and stability of somaclonal variation in micropropagated strawberry. Acta Horticuturae (ISHS), 280: 375–384.Sowik I., Michalczuk L. (2002): Zróżnicowanie molekularne w populacji mikrorozmnażanych truskawek w zależności od sposobu prowadzenia kultury. Zeszyty Problemowe Postępów Nauk Rolniczych, 488/II: 645–650.Sowik I., Michalczuk L. (2007): Somaclonal variation in plant in vitro cultures as a tool to generate strawberry genotypes resistant to Verticillium dahliae. In: 7th International Symposium Recent advances in plant biotechnology, June 10–16, 2007. Stará Lesná, Slovak Republic.Sowik Iwona, Bielenin Anna, Michalczuk Lech (2001): In vitro testing of strawberry resistance to Verticillium dahliae and Phytophthora cactorum. Scientia Horticulturae, 88, 31-40 https://doi.org/10.1016/S0304-4238(00)00195-3Sowik I., Wawrzyńczak D., Michalczuk L. (2003): Ex vitro establishment and greenhouse performance of somaclonal variants of strawberry selected for resistance to Verticillium dahliae. Acta Horticulturae (ISHS), 616: 497–500.Sowik I., Borkowska B., Wawrzyńczak D., Michalczuk L. (2004a): Morphological characteristics and photosynthetic activity of selected strawberry clone with enhanced resistance to Verticillium wilt. Acta Horticulturae (ISHS), 649: 181–184.Sowik I., Wawrzyńczak D., Michalczuk L. (2004b): Selection of somaclonal variants of strawberry with increased tolerance to Verticillium wilt. Scientific Works of Lithuanian Institute of Horticulture and Lithuanian University of Agriculture. Horticulture and Vegetable Growing, 23: 164–173.Takahashi Harumi, Furuya Hiromitsu, Takai Takatsugu, Matsumoto Tsutomu (1997): Characteristics of Alternaria alternata Strawberry Pathotype Isolated in New Zealand and the Resistance of the 'Akita Berry' Strawberry to the Fungus. Engei Gakkai zasshi, 65, 785-790 https://doi.org/10.2503/jjshs.65.785Talboys P. W. (1960): A CULTURE-MEDIUM AIDING THE IDENTIFICATION OF VERTICILLIUM ALBO-ATRUM AND V. DAHLIAE. Plant Pathology, 9, 57-58 https://doi.org/10.1111/j.1365-3059.1960.tb01147.xToyoda Hideyoshi, Horikoshi Koji, Yamano Yasuyoshi, Ouchi Seiji (1991): Selection for Fusarium wilt disease resistance from regenerants derived from leaf callus of strawberry. Plant Cell Reports, 10, - https://doi.org/10.1007/BF00234287van Dijk Thijs, Pagliarani Giulia, Pikunova Anna, Noordijk Yolanda, Yilmaz-Temel Hulya, Meulenbroek Bert, Visser Richard GF, van de Weg Eric (2014): Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map. BMC Plant Biology, 14, 55- https://doi.org/10.1186/1471-2229-14-55Vázquez Ana M. (2001): Insight into somaclonal variation. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 135, 57-62 https://doi.org/10.1080/11263500112331350650Wilhelm S. (1955): Longevity of the Verticillium wilt fungus in the laboratory and in the field. Phytopathology, 45: 180–181.Żebrowska Jadwiga I. (2012): Efficacy of resistance selection to Verticillium wilt in strawberry (Fragaria x ananassa Duch.) tissue culture. Acta Agrobotanica, 64, 3-12 https://doi.org/10.5586/aa.2011.024