Changes in gene expression profile during fruit development determine fruit quality

https://doi.org/10.17221/78/2015-HORTSCICitation:Keller-Przybyłkowicz S., Rutkowski K.P., Kruczyńska D.E., Pruski K. (2016): Changes in gene expression profile during fruit development determine fruit quality. Hort. Sci. (Prague), 43: 1-9.
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Climacteric fruit maturation is polygenic, complex process. Gene activity has a significant effect on the quality characteristics of the fruit for harvest and storage. Existing methods generally allow determining the degree of ripeness at harvest (point ‘0’). Since there is no method defining onset of the climacteric stage of the fruits, an attempt to identify the functional molecular marker that would determine a physiological ripeness of the fruit several days in advance before harvest was conducted. The analysis of changes in transcript of ten selected genes, and evaluation of the correlation of these genes with changes in fruits quality of two apple varieties Golden Delicious (winter cv.) and McIntosh (autumn cv.), allowed to identify a potential marker, activated a few days before harvesting the fruits. Overexpression of the starch glucosidase gene (StG) in the late fruits has been observed prior to the onset of ethylene production. The results confirm that it could be a potential functional marker useful for assessment of physiological ripeness status of cvs Golden Delicious and McIntosh.
References:
Bai Yang, Dougherty Laura, Xu Kenong (2014): Towards an improved apple reference transcriptome using RNA-seq. Molecular Genetics and Genomics, 289, 427-438  https://doi.org/10.1007/s00438-014-0819-3
 
Barry S.C., Liop-Tous I.M, Grierson D. 2000. The regulation of 1aminocyclopropane-1carboxylic acid synthase gene expression during the transistion from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology. 123: 979–986.
 
Blažek J., Hlušičková I., Varga A. (2003): Changes in quality characteristics of Golden Delicious apples under different storage conditions and correlations between them. Horticultural Science (Prague), 30: 81–89.
 
Cevik Volkan, Ryder Carol D., Popovich Alexandra, Manning Kenneth, King Graham J., Seymour Graham B. (2010): A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.). Tree Genetics & Genomes, 6, 271-279  https://doi.org/10.1007/s11295-009-0247-4
 
Costa Fabrizio, Alba Rob, Schouten Henk, Soglio Valeria, Gianfranceschi Luca, Serra Sara, Musacchi Stefano, Sansavini Silviero, Costa Guglielmo, Fei Zhangjun, Giovannoni James (2010): Use of homologous and heterologous gene expression profiling tools to characterize transcription dynamics during apple fruit maturation and ripening. BMC Plant Biology, 10, 229-  https://doi.org/10.1186/1471-2229-10-229
 
Giovannoni J. J. (2004): Genetic Regulation of Fruit Development and Ripening. THE PLANT CELL ONLINE, 16, S170-S180  https://doi.org/10.1105/tpc.019158
 
Goulao Luis F., Oliveira Cristina M. (2007): Molecular identification of novel differentially expressed mRNAs up-regulated during ripening of apples. Plant Science, 172, 306-318  https://doi.org/10.1016/j.plantsci.2006.09.006
 
Han Sang Eun, Seo Young Sam, Heo Seong, Kim Daeil, Sung Soon-Kee, Kim Woo Taek (2008): Structure and expression of MdFBCP1, encoding an F-box-containing protein 1, during Fuji apple (Malus domestica Borkh.) fruit ripening. Plant Cell Reports, 27, 1291-1301  https://doi.org/10.1007/s00299-008-0555-4
 
Hancock J.F., Luby J.J., Brown S.K., Lobos G.A. (2008): Apples. In: Hancock J.F. (ed.): Temperate Fruit Crop Breeding, Germplasm to Genomics. Springer Science USA, Business Media B.V.: 1–38.
 
Ireland Hilary S., Yao Jia-Long, Tomes Sumathi, Sutherland Paul W., Nieuwenhuizen Niels, Gunaseelan Kularajathevan, Winz Robert A., David Karine M., Schaffer Robert J. (2013): Apple SEPALLATA1/2 -like genes control fruit flesh development and ripening. The Plant Journal, 73, 1044-1056  https://doi.org/10.1111/tpj.12094
 
Janssen Bart J, Thodey Kate, Schaffer Robert J, Alba Rob, Balakrishnan Lena, Bishop Rebecca, Bowen Judith H, Crowhurst Ross N, Gleave Andrew P, Ledger Susan, McArtney Steve, Pichler Franz B, Snowden Kimberley C, Ward Shayna (2008): Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biology, 8, 16-  https://doi.org/10.1186/1471-2229-8-16
 
Langenkämper G., McHale R., Gardner R.C., MacRae E. (1998): Sucrose-phosphate synthase steady-state mRNA increases in ripening kiwifruit. Plant Molecular Biology, 36: 857–869. https://doi.org/10.1023/A:1005964812161
 
Larionov A., Andreas Krause A., Miller W. (2005): A standard curve based method for relative real time PCR data processing. BMC Bioinformatics, 6: 62.  https://doi.org/10.1186/1471-2105-6-62
 
Lee Young-Pyo, Yu Gyung-Hee, Seo Young Sam, Han Sang Eun, Choi Yeon-Ok, Kim Daeil, Mok Il-Gin, Kim Woo Taek, Sung Soon-Kee (2007): Microarray analysis of apple gene expression engaged in early fruit development. Plant Cell Reports, 26, 917-926  https://doi.org/10.1007/s00299-007-0308-9
 
Lelievre Jean-Marc, Latche Alain, Jones Brian, Bouzayen Mondher, Pech Jean-Claude (1997): Ethylene and fruit ripening. Physiologia Plantarum, 101, 727-739  https://doi.org/10.1111/j.1399-3054.1997.tb01057.x
 
Li M., Feng F., Cheng L. (2012): Expression patterns of genes involved in sugar methabolism and accumulation during fruit development. PLoS ONE, 7: 1–14.
 
Malladi A., Johnson L. K. (): Expression profiling of cell cycle genes reveals key facilitators of cell production during carpel development, fruit set, and fruit growth in apple (Malusxdomestica Borkh.). Journal of Experimental Botany, 62, 205-219  https://doi.org/10.1093/jxb/erq258
 
Park S. (2006): Identification of Genes with Potential Roles in Apple Fruit Development and Biochemistry through Large-Scale Statistical Analysis of Expressed Sequence Tags. PLANT PHYSIOLOGY, 141, 811-824  https://doi.org/10.1104/pp.106.080994
 
Payasi Anurag, Mishra Nagendra Nath, Chaves Ana Lucia Soares, Singh Randhir (2009): Biochemistry of fruit softening: an overview. Physiology and Molecular Biology of Plants, 15, 103-113  https://doi.org/10.1007/s12298-009-0012-z
 
Percy Ann E., O'Brien Iona E. W., Jameson Paula E., Melton Laurence D., MacRae ELspeth A., Redgwell Robert J. (1996): Xyloglucan endotransglycosylase activity during fruit development and ripening of apple and kiwifruit. Physiologia Plantarum, 96, 43-50  https://doi.org/10.1111/j.1399-3054.1996.tb00181.x
 
SAPERS G. M., ABBOTT J., MASSIE O., WATADA A., FINNEY E. E. (1977): VOLATILE COMPOSITION OF MCINTOSH APPLE JUICE AS A FUNCTION OF MATURITY AND RIPENESS INDICES. Journal of Food Science, 42, 44-47  https://doi.org/10.1111/j.1365-2621.1977.tb01214.x
 
Seo Young Sam, Kim Woo Taek (2009): A Genomics Approach Using Expressed Sequence Tags and Microarrays in Ripening Apple Fruit (Malus domestica Borkh.). Journal of Plant Biology, 52, 35-40  https://doi.org/10.1007/s12374-008-9009-3
 
Shi Y., Jiang L., Zhang L., Kang R., Yu Z. 2013. Dynamic changes in proteins during apple (Malus × domestica) fruit ripening and storage. Horticulture Research, 1: 1–21.
 
Shmulevich I., Zhang W. (2002): Binary analysis and optimization-based normalization of gene expression data. Bioinformatics, 18, 555-565  https://doi.org/10.1093/bioinformatics/18.4.555
 
Soglio V., Costa F., Molthoff J. W., Weemen-Hendriks W. M. J., Schouten H. J., Gianfranceschi L. (2009): Transcription analysis of apple fruit development using cDNA microarrays. Tree Genetics & Genomes, 5, 685-698  https://doi.org/10.1007/s11295-009-0219-8
 
Tacken E., Ireland H., Gunaseelan K., Karunairetnam S., Wang D., Schultz K., Bowen J., Atkinson R. G., Johnston J. W., Putterill J., Hellens R. P., Schaffer R. J. (): The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit Softening. PLANT PHYSIOLOGY, 153, 294-305  https://doi.org/10.1104/pp.109.151092
 
Tanksley S. D. (2004): The Genetic, Developmental, and Molecular Bases of Fruit Size and Shape Variation in Tomato. THE PLANT CELL ONLINE, 16, S181-S189  https://doi.org/10.1105/tpc.018119
 
Thammawong Manasikan, Arakawa Osamu (2007): Starch Degradation of Detached Apple Fruit in Relation to Ripening and Ethylene. Journal of the Japanese Society for Horticultural Science, 76, 345-350  https://doi.org/10.2503/jjshs.76.345
 
Tschopp Patrick, Sherratt Emma, Sanger Thomas J., Groner Anna C., Aspiras Ariel C., Hu Jimmy K., Pourquié Olivier, Gros Jérôme, Tabin Clifford J. (): A relative shift in cloacal location repositions external genitalia in amniote evolution. Nature, 516, 391-394  https://doi.org/10.1038/nature13819
 
Wang L., Cui N., Zhang K-Y., Fan H-Y., Li T-L. (2013): Research advance of sucrose phosphate synthase (SPS) in higher plant. International Journal of Agriculture and Biology, 15: 1221–1226.
 
Zeng Ying, Yang Tao (2002): RNA isolation from highly viscous samples rich in polyphenols and polysaccharides. Plant Molecular Biology Reporter, 20, 417-417  https://doi.org/10.1007/BF02772130
 
Zhu Yanmin, Barritt Bruce H. (2008): Md-ACS1 and Md-ACO1 genotyping of apple (Malus x domestica Borkh.) breeding parents and suitability for marker-assisted selection. Tree Genetics & Genomes, 4, 555-562  https://doi.org/10.1007/s11295-007-0131-z
 
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