Stimulation of ex vitro growth of Rhododendron hybrids ‘Nova Zembla’ and ‘Alfred’ by inoculation of roots with Serendipita indica

https://doi.org/10.17221/7/2020-HORTSCICitation:

Trzewik A., Orlikowska T., Kowalczyk W., Maciorowski R., Ciołakowska-Marasek A., Klocke E. (2020): Stimulation of ex vitro growth of Rhododendron hybrids ‘Nova Zembla’ and ‘Alfred’ by inoculation of roots with Piriformospora indica. Hort. Sci. (Prague), 47: 194–202. 

download PDF

Experiments were carried out to study the consequences of inoculating Rhododendron ‘Nova Zembla’ and ‘Alfred’ microcuttings and young plants with Serendipita indica (formerly Piriformospora indica), an endophytic fungus. The inoculation at the in vitro rooting stage decreased the in vitro root and shoot quality, but after planting microplants in a greenhouse in a non-fertilised peat substrate, the plants grew 33% taller, with 10% more leaves and with more abundant roots in comparison with the non-inoculated microplants. The young plants rooted in vitro, acclimated in a greenhouse in a peat substrate and inoculated with S. indica at the time of the transplantation to the pots displayed, after 15 months, an increase in the shoot lengths by 35% and 13%, depending on the cultivar, in the leaf number by 47% and in the chlorophyll content by 31% when compared with the non-inoculated plants. The beneficial effect of S. indica on the young Rhododendron plants indicates the possibility of its practical use in the nursery production of this plant.

References:
Achatz B., Van Rüden S., Andrade D., Neumann E., Pons Kühnemann J., Kogel K.-H., Franken P., Waller F. (2010): Root colonization by Piriformospora indica enhances grain yield in barley under diverse nutrient regimes by accelerating plant development. Plant Soil, 333: 59–70. https://doi.org/10.1007/s11104-010-0319-0
 
Ansari M.W., Trivedi D.K., Sahoo R.K., Gill S.S., Tuteja N. (2013): A critical review on fungi mediated plant responses with special emphasis to Piriformospora indica on improved production and protection of crops. Plant Physiology and Biochemistry, 70: 403–417. https://doi.org/10.1016/j.plaphy.2013.06.005
 
Bagde U.S., Prasad R., Varma A. (2011): Influence of culture filtrate of Piriformospora indica on growth and yield of seed oil in Helianthus annus. Symbiosis, 53: 83–88. https://doi.org/10.1007/s13199-011-0114-6
 
Bajaj R., Hu W., Huang Y.Y., Prasad R., Varma A., Bushley K.E. (2015): The beneficial root endophyte Piriformospora indica reduces egg density of the soybean cyst nematode. Biological Control, 90: 193–199. https://doi.org/10.1016/j.biocontrol.2015.05.021
 
Barazani O., Benderoth M., Groten K., Kuhlemeier C., Baldwin I.T. (2005): Piriformospora indica and Sebacina vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuata. Oecologia, 146: 234–243. https://doi.org/10.1007/s00442-005-0193-2
 
Das A., Tripathi S., Varma A. (2017): Use of Piriformospora indica as potential biological hardening agent for endangered micropropagated Picrorhiza kurroa Royel ex Benth. Proceedings of the National Academy of Sciences, India, 87: 799–805.
 
Dirr M.A. (1998): Manual of Woody Landscape Plants: Their Identification, Ornamental Characteristics, Culture, Propagation and Uses. 5th Ed. Stipes Publishing L.L.C, Champaign, Illinois.
 
Dolatabadi H.K., Goltapeh E.M. (2013): Effect of inoculation with Piriformospora indica and Sebacina vermifera on growth of selected Brassicaceae plants under greenhouse conditions. Journal of Horticultural Research, 21: 115–124. https://doi.org/10.2478/johr-2013-0030
 
Druege U., Baltruschat H., Franken P. (2007): Piriformospora indica promotes adventitious root formation in cuttings. Scientia Horticulturae, 112: 422–426. https://doi.org/10.1016/j.scienta.2007.01.018
 
Fakhro A., Andrade-Linares D.R., Von Bargen S., Bandte M., Büttner C., Grosch R., Schwarz D., Franken P. (2010): Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza, 20: 191–200. https://doi.org/10.1007/s00572-009-0279-5
 
Franken P. (2012): The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind. Applied Microbiology and Biotechnology, 96: 1455–1464. https://doi.org/10.1007/s00253-012-4506-1
 
Gupta R.K., Verma V.S., Bhushan A., Raina V. (2017): Biological hardening of micropropagated tomato plantlets: a case study with Piriformospora indica. In: Varma A., Prasad R., Tuteja N. (eds): Mycorrhiza – Nutrient Uptake, Biocontrol, Ecorestoration, Springer Intern. Publishing AG: 301–311.
 
Hill T.W., Käfer E. (2001): Improved protocols for aspergillus medium: trace elements and minimum medium salt stock solutions. Fungal Genetics News Letter, 48: 20–21. https://doi.org/10.4148/1941-4765.1173
 
Hosseini F., Mosaddeghi M.R., Dexter A.R. (2017): Effect of the fungus Piriformospora indica on physiological characteristics and root morphology of wheat under combined drought and mechanical stresses. Plant Physiology and Biochemistry, 118: 107–120. https://doi.org/10.1016/j.plaphy.2017.06.005
 
Jacobs S., Zechmann B., Molitor A., Trujillo M., Petutschnig E., Lipka V., Kogel K.-H., Schäfer P. (2011): Broad-spectrum suppression of innate immunity is required for colonization of Arabidopsis roots by the fungus Pirimormospora indica. Plant Physiology, 156: 726–740. https://doi.org/10.1104/pp.111.176446
 
Johnson J.M., Alex T., Oelmüller R. (2014): Piriformospora indica: The versatile and multifunctional root endophytic fungus for enhanced yield and tolerance to biotic and abiotic stress in crop plants. Journal of Tropical Agriculture, 52: 103–122.
 
Johnson J.M., Sherameti I., Ludwig A., Nongbri P.L., Sun C., Lou B., Varma A., Oelmüller R. (2011): Protocols for Arabidopsis thaliana and Piriformospora indica co-cultivation – A model system to study plant beneficial traits. Journal of Endocytobiosis Cell Research, 21: 101–113.
 
Kumar M., Yadav V., Tuteja N., Johri A.K. (2009): Antioxidant enzyme activities in maize plants colonized with Piriformospora indica. Microbiology, 155: 780–790. https://doi.org/10.1099/mic.0.019869-0
 
Lee Y.-C., Johnson J.M., Cien C.-T., Sun C., Cai D., Lou B., Oelmüller R., Yeh K.-W. (2011): Growth promotion of Chinese cabbage and Arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin. Molecular Plant-Microbe Interactions, 24: 421–431. https://doi.org/10.1094/MPMI-05-10-0110
 
Lloyd G., McCown B. (1980): Commercially-feasible micropropagation of mountain laurer, Kalmia latifolia, by use shoot-tip cultures. Combined Proceedings, International Plant Propagators’ Society, 30: 421–427.
 
Malla R., Prasad R., Kumari R., Giang P.H., Pokharel U., Oelmüller R., Varma A. (2004): Phosphorus solubilizing symbiotic fungus: Piriformosfora indica. Endocytobiosis Cell Research, 15: 579–600.
 
Oelmüller R., Sherameti I., Tripathi S., Varma A. (2009): Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications. Symbiosis, 49: 1–7. https://doi.org/10.1007/s13199-009-0009-y
 
Official Methods of Analysis AOAC International, 16th Ed, Vol. 1, (1995): AOAC International, Arlington, Virginia, USA.
 
Qiang X., Weiss M., Kogel K.-H., Shäfer P. (2012): Piriformospora indica – a mutualistic basidiomycete with an exceptionally large plant host range. Molecular Plant Pathology, 13: 508–518. https://doi.org/10.1111/j.1364-3703.2011.00764.x
 
Rabiey M., Ullah I., Shaw L.J., Shaw M.W. (2017): Potential ecological effects of Piriformospora indica, a possible biocontrol agent, in UK agricultural system. Biological Control, 104: 1–9. https://doi.org/10.1016/j.biocontrol.2016.10.005
 
Rai M., Acharya D., Singh A., Varma A. (2001): Positive growth responses of the medicinal plants Spilanthes calva and Withania somnifera to inoculation by Piriformospora indica in a field trial. Mycorrhiza, 11: 123–128. https://doi.org/10.1007/s005720100115
 
Sahay N.S., Varma A. (1999): Piriformospora indica: a new biological hardening tool for micropropagated plants. FEMS Microbiology Letters, 181: 297–302. https://doi.org/10.1111/j.1574-6968.1999.tb08858.x
 
Serfling A., Wirsel S.G.R., Lind V., Deising H.B. (2007): Performance of the biocontrol fungus Piriformospora indica on wheat under greenhouse and field conditions. Phytopathology, 97: 523–531. https://doi.org/10.1094/PHYTO-97-4-0523
 
Sherameti I., Shahollari B., Venus Y., Altschmied L., Varma A., Oelmüller R. (2005): The endophytic fungus Piriformospora indica stimulates the expression of nitrate reductase and the starch-degrading enzyme glucan-water dikinase in tobacco and Arabidopsis roots through a homeodomain transcription factor that binds to a conserved motif in their promoters. Journal of Biological Chemistry, 280: 26241–26247. https://doi.org/10.1074/jbc.M500447200
 
Sirrenberg A., Göbel C., Grond S., Czempinski N., Ratzinger A., Karlovsky P., Santos P., Feussner I., Pawlowski K. (2007): Piriformospora indica affects plant growth by auxin production. Physiologia Plantarum, 131: 581–589. https://doi.org/10.1111/j.1399-3054.2007.00983.x
 
Sun C., Shao Y., Vahabi K., Lu J., Bhattacharya S., Dong S., Yeh K.-W., Sherameti I., Lou B., Baldwin I.T., Oelmüller R. (2014): The beneficial fungus Piriformospora indica protects Arabidopsis from Verticillium dahliae infection by downregulation plant defense responses. BMC Plant Biology, 14: 268. https://doi.org/10.1186/s12870-014-0268-5
 
Sun C., Johnson J.M., Cai D., Sherameti I., Oelmüller R., Lou B. (2010): Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastic-localized CAS protein. Journal of Plant Physiology, 167: 1009–1017. https://doi.org/10.1016/j.jplph.2010.02.013
 
Trzewik A., Maciorowski R., Klocke E., Orlikowska T. (2020): The influence of Piriformospora indica on the resistance of two rhododendron cultivars to Phytophthora cinnamomi and P. plurivora. Biological Control, 104: 104121. https://doi.org/10.1016/j.biocontrol.2019.104121
 
Tuladhar R., Shahi K., Shrestha S.M., Singh A., Varma A. (2017): Stimulated growth of Lycopersicon esculentum CLA 1131 in presence of Piriformospora indica and vermicompost. In: Varma A., Prasad R., Tuteja N. (eds): Mycorrhiza-Eco-Physiology, Secondary Metabolites, Nanomaterials. Springer Intern Publ AG: 233–245.
 
Varma A., Bakshi M., Lon B., Hartmann A., Oelmüller R. (2012): Piriformospora indica: a novel plant growth promoting mycorrhizal fungus. Agricultural Research, 1: 117–131. https://doi.org/10.1007/s40003-012-0019-5
 
Varma A., Chordia P., Bakshi M., Oelmüller R. (2013): Introduction to Sebacinales. In: Varma A., Kost G., Oelmüller R. (eds): Piriformospora indica. Sebacinales and Their Biotechnological Application. Springer-Verlag: 3–24.
 
Varma A., Verma S., Sahay S.N., Bütehorn B., Franken P. (1999): Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Applied and Environmental Microbiology, 65: 2741–2744. https://doi.org/10.1128/AEM.65.6.2741-2744.1999
 
Verma S., Varma A., Rexer K.-H., Hassel A., Kost G., Sarbhoy A., Bisen P., Bütehorn B., Franken P. (1998): Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus. Mycologia, 90: 896–903. https://doi.org/10.1080/00275514.1998.12026983
 
Vyas S., Nagori R., Purohit S.D. (2008): Root colonization and growth enhancement of micropropagated Feronia limonia (L.) Swingle by Piriformospora indica – a cultivable root endophyte. International Journal of Plant Developmental Biology, 2: 128–132.
 
Walinga I., van der Lee J.J., Houba V.J.G., van Vark W. (1989): Plant analysis procedures. Part 7 Department of Soil Science and Plant Nutrition: 263.
 
Weiß M., Waller F., Zuccaro A., Selosse M.-A. (2016): Sebacinales – one thousand and one interactions with land plants. New Phytologist, 211: 20–40. https://doi.org/10.1111/nph.13977
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti