Sucrase in immobilized cells of Cucumis sativus L.

S.I. Ignatova, L.M. Kušč, L. Bilisics, L. Trinh, N.N. Tiuliaeva, M.G. Sedova

https://doi.org/10.17221/4465-HORTSCICitation:Ignatova S.I., Kušč L.M., Bilisics L., Trinh L., Tiuliaeva N.N., Sedova M.G. (2002): Sucrase in immobilized cells of Cucumis sativus L. Hort. Sci. (Prague), 29: 17-22.
download PDF

Cell suspension cultures of Cucumis sativus L. – cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53°C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.

Keywords:

glutaraldehyde; sucrase; cell permeabilization; cell immobilization; Cucumis sativus L.

download PDF

Impact Factor (Web of Science):

2018: 0.623
Q3 – Horticulture
5-Year Impact Factor: 0.719

SCImago Journal Rank (SCOPUS):

SCImago Journal & Country Rank

Similarity Check

All the submitted manuscripts are checked by the CrossRef Similarity Check.

New Issue Alert

Join the journal on Facebook!

Referred to in

Agrindex of Agris/FAO database
BIOSIS Previews
CAB Abstracts
CNKI
Czech Agricultural and Food Bibliography
DOAJ (Directory of Open Access Journals)
EBSCO – Academic Search Ultimate
EMBiology
Google Scholar
Horticulturae Abstracts
ISI Web of KnowledgeSM
J-GATE
Plant Breeding Abstracts
Science Citation Index Expanded®
SCOPUS
Web of Science®

Licence terms

All content is made freely available for non-commercial purposes, users are allowed to copy and redistribute the material, transform, and build upon the material as long as they cite the source.

Open Access Policy

This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.

Contact

Ing. Eva Karská
Executive Editor
phone: + 420 227 010 606
e-mail: hortsci@cazv.cz

Address

Horticultural Science
Czech Academy of Agricultural Sciences
Slezská 7, 120 00 Praha 2, Czech Republic

© 2019 Czech Academy of Agricultural Sciences