Sucrase in immobilized cells of Cucumis sativus L.
S.I. Ignatova, L.M. Kušč, L. Bilisics, L. Trinh, N.N. Tiuliaeva, M.G. Sedovahttps://doi.org/10.17221/4465-HORTSCICitation:Ignatova S.I., Kušč L.M., Bilisics L., Trinh L., Tiuliaeva N.N., Sedova M.G. (2002): Sucrase in immobilized cells of Cucumis sativus L. Hort. Sci. (Prague), 29: 17-22.
Cell suspension cultures of Cucumis sativus L. – cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53°C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.
glutaraldehyde; sucrase; cell permeabilization; cell immobilization; Cucumis sativus L.