The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones
H. Vlastníková, K. Moravcová, M. Pidrahttps://doi.org/10.17221/3807-HORTSCICitation:Vlastníková H., Moravcová K., Pidra M. (2004): The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones. Hort. Sci. (Prague), 31: 136-139.
Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Polešovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several of them gave different results from those expected: Pinot blanc sample 26 has the RAPD profile typical of Chardonnay. A new abnormal RAPD pattern as a marker of typical Chardonnay and Pinot profiles was observed in two cases. While RAPD banding patterns could not distinguish between the known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.Keywords:
grapevine; clones; fingerprinting; RAPD