The reliability of detection and the distribution of Apple chlorotic leafspot virus in pears in the Czech Republic
J. Polák, J. Svobodahttps://doi.org/10.17221/3733-HORTSCICitation:Polák J., Svoboda J. (2006): The reliability of detection and the distribution of Apple chlorotic leafspot virus in pears in the Czech Republic. Hort. Sci. (Prague), 33: 7-10.
The distribution of Apple chlorotic leafspot virus in pear cultivars in selected orchards of three different districts in the Czech Republic was evaluated by ELISA. The same procedure of DAS – ELISA as in apple trees, was used for the detection of ACLSV in pear trees. The detection of ACLSV in pear flower petals by ELISA was more sensitive than the ACLSV detection in leaves. ACLSV was detected in pear leaves in the reliable way only in June. The presence of ACLSV was not proved by ELISA in nine tested pear rootstocks. The different distribution of ACLSV was detected in pear cultivars Lucasova máslovka and Boscova lahvice grown in different orchards. Low occurrence of ACLSV was found in cultivars Konference, Pařížanka and Dicolor. ACLSV was not detected in cultivars Madame Verté and Bohemica.Keywords:
pear; orchards; Apple chlorotic leafspot virus; distribution; flower; cultivars and rootstocks; ELISA