In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants
S. Kashyap, S. Sharmahttps://doi.org/10.17221/3743-HORTSCICitation:Kashyap S., Sharma S. (2006): In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants. Hort. Sci. (Prague), 33: 77-86.
In vitro selected salt tolerant saplings of Morus alba (cv. Sujanpuri) were raised from nodal explants with axillary buds collected during three different periods of the year. The growth and shoot/root multiplication of the nodal explants collected between November to February and July to October were found to be better than those collected between March to June. In cultures, shoot multiplication was induced by the application of 2.5 mg/l of 6-benzylaminopurine (BAP) and 0.3 mg/l of gibberellic acid (GA3), while rooting by 1.0 mg/l of indolebutyric acid (IBA). Sodium chloride (NaCl) was added to induce salt stress and its concentration was gradually increased from 0.1% (w/v) onwards. The salt tolerance was observed up to 0.4% (w/v) NaCl and 100% mortality of explants was noted above this concentration. The inclusion of Arbuscular Mycorrhizal (AM) fungi and Azotobacter chroococcum to tissue culture of raised saplings during acclimatization enhanced their survival and resulted in a significant increase of plant growth. After the transfer of plants to salt affected wasteland, only NaCl-treated saplings survived, whereas those developed without NaCl resulted in 100% mortality.Keywords:
in vitro; mulberry; NaCl; AM fungi; Azotobacter