Distribution of viruses in the shallot germplasm collection of the Czech Republic – Short Communication
Kateřina Smékalová, Helena Stavělíková, Karel Dušekhttps://doi.org/10.17221/271/2015-HORTSCICitation:Smékalová K., Stavělíková H., Dušek K. (2017): Distribution of viruses in the shallot germplasm collection of the Czech Republic – Short Communication. Hort. Sci. (Prague), 44: 49-52.
The Czech collection of shallot (Allium cepa var. ascalonicum) genetic resources (122 accessions) was surveyed for the presence of four different viruses, i.e. Onion yellow dwarf virus, Leek yellow stripe virus, Garlic common latent virus (GCLV), and Shallot latent virus, by DAS-ELISA. The shallot seems to be resistant against GCLV because none of the tested plants was infested by this virus. Other three viruses were found with an incidence ranging from 53% to 93% for genotypes and 48% to 87% for plants. Most of the tested shallot genotypes were simultaneously infected with two or three viruses. These results were compared with neighbouring collection of garlic where all four viruses were found widespread with an incidence ranging from 65% to 83% for genotypes and 39% to 61% for plants.Keywords:
Allium cepa var. ascalonicum; ELISA; OYDV; LYSV; GCLV; SLV
Alves-Júnior Miguel, Menezes Marraccini Fernanda, de Albuquerque Melo Filho Péricles, Nepomuceno Dusi André, Pio-Ribeiro Gilvan, Morais Ribeiro Bergmann (2008): Recombinant expression of Garlic virus C (GARV-C) capsid protein in insect cells and its potential for the production of specific antibodies. Microbiological Research, 163, 354-361 https://doi.org/10.1016/j.micres.2006.06.016Cafrune E. E., Perotto M. C., Conci V. C. (2006): Effect of Two Allexivirus Isolates on Garlic Yield. Plant Disease, 90, 898-904 https://doi.org/10.1094/PD-90-0898Chen Jiong, Chen Jien Ping, Adams M. J. (2002): Characterisation of some carla- and potyviruses from bulb crops in China. Archives of Virology, 147, 419-428 https://doi.org/10.1007/s705-002-8330-yConci Vilma C., Lunello Pablo, Buraschi Diana, Italia Rusell R., Nome Sergio F. (2002): Variations of Leek yellow stripe virus Concentration in Garlic and Its Incidence in Argentina. Plant Disease, 86, 1085-1088 https://doi.org/10.1094/PDIS.2002.86.10.1085Conci Vilma C., Canavelli Ana, Lunello P., Di Rienzo J., Nome S. F., Zumelzu G., Italia Rusell (2003): Yield Losses Associated with Virus-Infected Garlic Plants During Five Successive Years. Plant Disease, 87, 1411-1415 https://doi.org/10.1094/PDIS.2003.87.12.1411Diekmann M. (1997): Allium spp. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm No. 18.Dovas C.I., Hatziloukas E., Solomon R., Barg E., Shiboleth Y., Katis N. (2001): Incidence of viruses infecting Allium spp. in Greece. European Journal of Plant Pathology, 107: 677–684. https://doi.org/10.1023/A:1011958914573Fletcher P. J., Fletcher J. D., Lewthwaite S. L. (1998): In vitro elimination of onion yellow dwarf and shallot latent viruses in shallots ( Allium cepa var. ascalonicum L.). New Zealand Journal of Crop and Horticultural Science, 26, 23-26 https://doi.org/10.1080/01140671.1998.9514035Keller E.R.J., Senula A. (2001): PROGRESS IN STRUCTURING AND MAINTAINING THE GARLIC (Allium sativum) DIVERSITY FOR THE EUROPEAN GENRES PROJECT. Acta Horticulturae, , 189-193 https://doi.org/10.17660/ActaHortic.2001.555.28Lot Hervé, Chovelon Véronique, Souche Sylvie, Delecolle Brigitte (1998): Effects of Onion Yellow Dwarf and Leek Yellow Stripe Viruses on Symptomatology and Yield Loss of Three French Garlic Cultivars. Plant Disease, 82, 1381-1385 https://doi.org/10.1094/PDIS.19188.8.131.521Lunello P., Di Rienzo J., Conci V. C. (2007): Yield Loss in Garlic Caused by Leek yellow stripe virus Argentinean Isolate. Plant Disease, 91, 153-158 https://doi.org/10.1094/PDIS-91-2-0153Y.M. Mahmoud Sabry, A. Abo-El Maaty Sabah, M. El-Borollosy Ali, H. Abdel-Ghaffar Mamdouh (2008): Identification of Onion yellow dwarf potyvirus as One of the Major Viruses Infecting Garlic in Egypt. International Journal of Virology, 4, 1-13 https://doi.org/10.3923/ijv.2008.1.13Rabinowitch H.D., Kamenetsky R. (2002): Shallot (Allium cepa, Aggregatum Group). In: Rabinowitch H.D., Currah L. (eds.): Allium crop science: recent advances. Wallingford, CABI Publishing.Smékalová K., Stavělíková H., Dušek K. (2010): Distribution of viruses in the garlic germplasm collection of the Czech Republic. Journal of Plant Pathology, 96: 273–274.Takaichi Miyuki, Nagakubo Takayuki, Oeda Kenji (2001): Mixed Virus Infections of Garlic Determined by a Multivalent Polyclonal Antiserum and Virus Effects on Disease Symptoms. Plant Disease, 85, 71-75 https://doi.org/10.1094/PDIS.2001.85.1.71Dijk P. (1993): Survey and characterization of potyviruses and their strains of Allium species. Netherlands Journal of Plant Pathology, 99, 1-48 https://doi.org/10.1007/BF02017734Walkey D. G. A., Antill D. N. (2015): Agronomic evaluation of virus-free and virus-infected garlic ( Allium sativum L.). Journal of Horticultural Science, 64, 53-60 https://doi.org/10.1080/14620316.1989.11515927Walkey D. G. A., Webb M. J. W., Bolland C. J., Miller A. (2015): Production of virus-free garlic ( Allium sativum L.) and shallot ( A. ascalonicum L.) by meristem-tip culture. Journal of Horticultural Science, 62, 211-220 https://doi.org/10.1080/14620316.1987.11515771Ward L. I., Perez-Egusquiza Z., Fletcher J. D., Clover G. R. G. (2009): A survey of viral diseases of Allium crops in New Zealand. Australasian Plant Pathology, 38, 533- https://doi.org/10.1071/AP09039