Morphological variability between diploid and tetraploid taxa of the genus Betula L. in the Czech Republic R., Kuneš I., Baláš M., Gallo J. (2017): Morphological variability between diploid and tetraploid taxa of the genus Betula L. in the Czech Republic. J. For. Sci., 63: 531-537.
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The aim of this work was to suggest a reliable method for distinguishing between diploid and tetraploid taxa of the genus Betula Linnaeus, based on leaf measurements. In total, 97 individuals from 6 locations within the Bohemian Forest region (Czech Republic) were selected. Four leaves from each individual were evaluated. On each leaf, twenty parameters were measured. Each sample was analysed by flow cytometry to determine its actual ploidy. Measured parameters were analysed by principal component analysis and tested for differences between diploid and tetraploid taxa. For actual ploidy prediction, a classification function was designed. The reliability of the classification function was verified on samples from three different regions of the Czech Republic and compared with functions as suggested by other authors. The classification function designed in this work (based on 3 parameters – blade width in the upper 1/4 of blade length, first vein angle and number of leaf teeth between 3rd and 4th vein) correctly determined actual ploidy in 89% of all tested samples.
Ahdesmäki M., Zuber V., Gibb S., Strimmer K. (2015): Sda: Shrinkage discriminant analysis and CAT score variable selection. Available at
Ashburner K., McAllister H.A. (2013): The Genus Betula: A taxonomic Revision of Birches. Richmond, Kew Publishing: 300.
Atkinson M.D., Codling A.N. (1986): A reliable method for distinguishing between Betula pendula and B. pubescens. Watsonia, 16: 75–87.
Balcar V. (2001): Some experience with European birch (Betula pendula) and Carpathian birch (Betula carpatica) planted on the ridge part of Jizerské hory Mts. Journal of Forest Science, 47 (Special Issue): 150–155.
Chytrý M., Kučera T., Kočí M., Grulich V., Lustyk P. (eds) (2010): Katalog biotopů České republiky. 2nd Ed. Prague, Agentura ochrany přírody a krajiny České republiky: 445.
Ešnerová J., Karlík P., Zahradník D., Koňasová T., Stejskal J., Baláš M., Vítámvás J., Rašáková N., Stacho J., Kuthan J., Lukášová M., Kuneš I. (2012): Morfologická variabilita rodu bříza (Betula L.) v Krkonoších se zaměřením na tetraploidní zástupce. Zprávy lesnického výzkumu, 57: 112–125.
Gardiner A.S. (1972): A review of the sub-species carpatica and tortuosa within the species betula pubescens Ehrh.. Transactions of the Botanical Society of Edinburgh, 41, 451-459
Gardiner A.S., Jeffers J.N.R. (1962): Analysis of the collective species Betula alba L. on the basis of leaf measurements. Silvae Genetica, 11: 125–176.
Grimm Guido W., Renner Susanne S. (2013): Harvesting Betulaceae sequences from GenBank to generate a new chronogram for the family. Botanical Journal of the Linnean Society, 172, 465-477
HOWLAND D. E., OLIVER R. P., DAVY A. J. (1995): Morphological and molecular variation in natural populations of Betula. New Phytologist, 130, 117-124
Hynynen J., Niemisto P., Vihera-Aarnio A., Brunner A., Hein S., Velling P. (2010): Silviculture of birch (Betula pendula Roth and Betula pubescens Ehrh.) in northern Europe. Forestry, 83, 103-119
Institute of Botany of the CAS (2010): Virtuální floristická kartotéka. Available at (accessed Jan 1, 2017).
Jarvinen P., Palme A., Orlando Morales L., Lannenpaa M., Keinanen M., Sopanen T., Lascoux M. (2004): Phylogenetic relationships of Betula species (Betulaceae) based on nuclear ADH and chloroplast matK sequences. American Journal of Botany, 91, 1834-1845
Kříž Z. (1990): Betula L. – bříza. In: Hejný S., Slavík B. (eds): Květena České republiky 2. Prague, Academia: 36–46.
Kula E. (2011): Bříza a její význam pro trvalý rozvoj lesa v imisních oblastech. Kostelec nad Černými lesy, Lesnická práce, s.r.o.: 276.
Kuneš I., Balcar V., Zahradník D. (2007): Influence of a planting hole application of dolomitic limestone powder and basalt grit on the growth of Carpathian birch (Betula carpatica W. et K.) and soil chemistry in the air-polluted Jizerské hory Mts. Journal of Forest Science, 53: 505–515.
Meier-Dinkel A. (1992): Micropropagation of birches (Betula spp.). In: Bajaj Y.P.S. (ed.): High-Tech and Micropropagation II. Berlin, Heidelberg, Springer-Verlag: 40–81.
Otto F. (1990): DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. Methods in Cell Biology, 33: 105–110.
Palme A. E., Su Q., Palsson S., Lascoux M. (2004): Extensive sharing of chloroplast haplotypes among European birches indicates hybridization among Betula pendula, B. pubescens and B. nana. Molecular Ecology, 13, 167-178
Podrázský V., Ulbrichová I. (2004): Restoration of forest soils on reforested abandoned agricultural lands. Journal of Forest Science, 50: 249–255.
Schönswetter Peter, Suda Jan, Popp Magnus, Weiss-Schneeweiss Hanna, Brochmann Christian (2007): Circumpolar phylogeography of Juncus biglumis (Juncaceae) inferred from AFLP fingerprints, cpDNA sequences, nuclear DNA content and chromosome numbers. Molecular Phylogenetics and Evolution, 42, 92-103
Suda J., Pyšek P. (2010): Flow cytometry in botanical research: Introduction. Preslia, 82: 1–2.
Sułowicz S., Płociniczak T., Piotrowska-Seget Z., Kozdrój J. (2011): Significance of silver birch and bushgrass for establishment of microbial heterotrophic community in a metal-mine spoil heap. Water, Air, & Soil Pollution, 214: 205–218.
Wielgolaski F.E. (2001): Nordic Mountain Birch Ecosystems. Paris, UNESCO: 390.
Zerbe S. (2001): On the ecology of Sorbus aucuparia (Rosaceae) with special regard to germination, establishment and growth. Polish Botanical Journal, 46: 229–239.
Zuber V., Strimmer K. (2009): Gene ranking and biomarker discovery under correlation. Bioinformatics, 25, 2700-2707
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