An assessment of biological control of Polygraphus major Stebbing, 1903 (Coleoptera: Curculionidae) by entomopathogenic fungi A.L., Buhroo A.A. (2018): An assessment of biological control of Polygraphus major Stebbing, 1903 (Coleoptera: Curculionidae) by entomopathogenic fungi. J. For. Sci., 64: 178-186.
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Recently the use of fungal entomopathogens against bark beetles has gained increasing attention throughout the world and researchers continue to seek highly pathogenic fungal isolates for controlling beetle pests. In the present study, the efficacy of three entomopathogenic fungi, namely Beauveria bassiana (Balsamo) Vuillemin, Metarhizium anisopliae sensu lato (Metchnikoff) Sorokin and Lecanicillium lecanii (Zimmerman) Zare & Gams, was tested against Polygraphus major Stebbing, 1903 under laboratory conditions. Each fungal suspension contained 1.0 × 109 spores of fungi in 1 ml. An insecticide – Cyclone was also used as positive control in the experiment. The mortality caused by these fungi was recorded in treated branches and petri plate assay. In treated branches, B. bassiana and M. anisopliae s. l. caused higher mortality, i.e. 57.77 and 46%, respectively, after 10 days of treatment and 98 and 92.77%, respectively, after 20 days of treatment. The results of the petri plate assay revealed that P. major adults were highly susceptible to both applied fungal species and insecticide. However, B. bassiana and M. anisopliae s. l. caused higher percentage mortalities after six days of treatment, i.e. 100 and 91.66%, respectively. The percentage mortality caused by application of the insecticide was 69%. L. lecanii was observed to be significantly less virulent (mortality 46.66%) in all fugal treatments. After observing the promising nature of the three entomopathogenic fungi by testing them, we arrive at the conclusion that the tested fungi have a potential for the control of P. major, and further field experiments are warranted to investigate their efficacy under more practical conditions.

Andrei A.M., Lupăştean D., Ciornei C., Fătu A.C., Dinu M.M. (2013): Laboratory Beauveria bassiana (Bals.) Vuill. bioassays on spruce bark beetle (Ips typographus L.). IOBC-WPRS Bulletin, 90: 93–96.
Bałazy S. (2015): Grzyb Cephalosporium (Acrostalagmus) Lecanii Zimm. - sprawca choroby larw chrząszczy [The fungus Cephalosporium (Acrostalagmus) lecanii Zimm. the pathogen for the larvae of beetles]. Acta Societatis Botanicorum Poloniae, 32, 69-80
Bałazy S. (1965): Entomopathogenous fungi from the order Hyphomycetes damaging forest insects in Poland. Roczniki Wyzszej Szkoly Rolniczej w Poznaniu, 27: 21–30.
BARSON G. (1976): Laboratory studies on the fungus Verticillium lecanii, a larval pathogen of the large elm bark beetle (Scolytus scolytus). Annals of Applied Biology, 83, 207-214
Batta Y.A. (2007): Biocontrol of almond bark beetle (Scolytus amygdali Geurin-Meneville, Coleoptera: Scolytidae) using Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes). Journal of Applied Microbiology, 103, 1406-1414
Buhroo A.A., Rasheed F.N., Khanday A.L. (2016): An assessment on population density of San Jose scale Quadraspidiotus perniciosus (Comstock) and its biological control in Kashmir (Hemiptera: Diaspididae). Munis Entomology & Zoology, 11: 434–440.
Bychawska S., Swiezynska H. (1979): Attempts to control Myelophilus piniperda L. by means of the entomopathogenic fungus Beauveria bassiana (Bals.). Vuill. Sylwan, 123: 59–64. (in Polish)
Dinu M.M., Lupăştean D., Cardaş G., Andrei A.M. (2012): New Beauveria bassiana (Bals.) Vuill. isolate from Ips duplicatus (Sahlberg). Romanian Journal of Plant Protection, 5: 12–15.
Gabarty A., Salem H.M., Fouda M.A., Abas A.A., Ibrahim A.A. (2014): Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon (Hufn.). Journal of Radiation Research and Applied Sciences, 7, 95-100
Grosman Donald M., Upton William W. (2006): Efficacy of Systemic Insecticides for Protection of Loblolly Pine Against Southern Pine Engraver Beetles (Coleoptera: Curculionidae: Scolytinae) and Wood Borers (Coleoptera: Cerambycidae). Journal of Economic Entomology, 99, 94-101
Humber R.A. (1997): Fungi: Identification. In: Lacey L.A. (ed.): Manual of Techniques in Insect Pathology. San Diego, Academic Press, Inc.: 153–185.
Inglis G.D., Goettel M.S., Butt T.M., Strasser H. (2001): Use of hyphomycetous fungi for managing insect pests. In: Butt T.M., Jackson C.W., Magan N. (eds): Fungi as Biocontrol Agents: Progress, Problems and Potential. Wallingford, CABI: 23–69.
Jakuš R., Blaženec M. (2011): Treatment of bark beetle attacked trees with entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin. Folia Forestalia Polonica, Series A – Forestry, 53: 150–155.
Jurc M. (2004): Insect pathogens with special reference to pathogens of bark beetles (Col., Scolytidae: Ips typographus L.). Preliminary results of isolation of entomopathogenic fungi from two spruce bark beetles in Slovenia. Zbornik gozdarstva in lesarstva, 74: 97–124.
Kirschner R. (2001): Diversity of filamentous fungi in bark beetle galleries in Central Europe. In: Misra J.K., Horn B.W. (eds): Trichomycetes and Other Fungal Groups: Professor Robert W. Lichtwardt Commemoration Volume. Enfield, B.W. Science Publishers, Inc.: 175–196.
Lieutier François, Yart Annie, Salle Aurélien (2009): Stimulation of tree defenses by Ophiostomatoid fungi can explain attack success of bark beetles on conifers. Annals of Forest Science, 66, 801-801
Lutyk P., Swiezynska H. (1984): Trials to control the larger pine-shoot beetle (Tomicus piniperda L.) with the use of the fungus Beauveria bassiana (Bals.) Vuill. on piled wood. Sylwan, 128: 41–45.
Maiti P.K., Saha N. (2009): The Fauna of India and the Adjacent Countries. Scolytidae: Coleoptera (Bark and Ambrosia Beetles). Volume: I (Part 2). Kolkata, Zoological Survey of India: 245.
Markova G. (2000): Pathogenicity of several entomogenous fungi to some of the most serious forest insect pests in Europe. IOBC-WPRS Bulletin, 23: 231–239.
Mudrončeková S., Mazáň M., Nemčovič M., Šalamon I. (2013): Entomopathogenic fungus species Beauveria bassiana (Bals.) and Metarhizium anisopliae (Metsch.) used as mycoinsecticide effective in biological control of Ips typographus (L.). Journal of Microbiology, Biotechnology and Food Sciences, 2: 2469–2472.
Murad A.M., Laumann R.A., Mehta A., Noronha E.F., Franco O.L. (2007): Screening and secretomic analysis of en-thomopatogenic Beauveria bassiana isolates in response to cowpea weevil (Callosobruchus maculatus) exoskeleton. Comparative Biochemistry and Physiology – Part C: Toxicology & Pharmacology, 145: 333–338.
Paine T. D., Raffa K. F., Harrington T. C. (1997): INTERACTIONS AMONG SCOLYTID BARK BEETLES, THEIR ASSOCIATED FUNGI, AND LIVE HOST CONIFERS. Annual Review of Entomology, 42, 179-206
Popa Valentin, Déziel Eric, Lavallée Robert, Bauce Eric, Guertin Claude (2012): The complex symbiotic relationships of bark beetles with microorganisms: a potential practical approach for biological control in forestry. Pest Management Science, 68, 963-975
Raffa K.F., Aukema B.H., Bentz B.J., Carroll A.L., Hicke J.A., Kolb T.E. (2015): Responses of tree-killing bark beetles to a changing climate. In: Björkman C., Niemelä P. (eds): Climate Change and Insect Pests. Wallingford, CABI: 173–201.
Rath Andrew C. (2000): The Use of Entomopathogenic Fungi for Control of Termites. Biocontrol Science and Technology, 10, 563-581
Roberts D.W. (1970): Coelomomyces, Entomophthora, Beauveria and Metarhizium as parasites of mosquitoes. Miscellaneous publications of the Entomological Society of America, 7: 140–155.
Schrank Augusto, Vainstein Marilene Henning (2010): Metarhizium anisopliae enzymes and toxins. Toxicon, 56, 1267-1274
Sindhu S.S., Rakshiya Y.S., Verma M.K. (2011): Biological control of termites by antagonistic soil microorganisms. In: Singh A., Parmar N., Kuhad R.C. (eds): Bioaugmentation, Biostimulation and Biocontrol. Berlin, Heidelberg, Springer: 261–309.
Stebbing E.P. (1914): Scotylidae. In: Indian Forest Insects of Economic Importance. Coleoptera. London, Eyre and Spot-tiswoode, Ltd.: 457– 610.
Steinwender B. M., Krenn H. W., Wegensteiner R. (2010): Different effects of the insectpathogenic fungus Beauveria bassiana (Deuteromycota) on the bark beetle Ips sexdentatus (Coleoptera: Curculionidae) and on its predator Thanasimus formicarius (Coleoptera: Cleridae). Journal of Plant Diseases and Protection, 117, 33-38
Vega F.E., Meyling N.V., Luangsaard J.J., Blackwell M. (2012): Fungal entomopathogens. In: Vega F.E., Kaya H.K. (eds): Insect Pathology. 2nd Ed. San Diego, Academic Press: 171–220.
Vincent C., Goettel M.S., Lazarovits G. (eds) (2007): Biological Control: A Global Perspective. Wallingford, CABI: 432.
Wegensteiner R. (1996): Laboratory evaluation of Beauveria bassiana (Bals.) Vuill. against Ips typographus (Coleoptera, Scolytidae). IOBC-WPRS Bulletin, 19: 186–189.
Wegensteiner R., Weiser J. (2004): Annual variation of pathogen occurrence and pathogen prevalence in Ips typographus (Coleoptera, Scolytidae) from the BOKU University Forest Demonstration Centre. Journal of Pest Science, 77, 221-228
Zhang Long-Wa, Liu Yu-Jun, Yao Jian, Wang Bin, Huang Bo, Li Zeng-Zhi, Fan Mei-Zhen, Sun Jiang-Hua (2011): Evaluation of Beauveria bassiana (Hyphomycetes) isolates as potential agents for control of Dendroctonus valens. Insect Science, 18, 209-216
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