Large bleeding lesions on stem and branches of pine trees, wet black spots and depressed cancer wounds of different sizes at the base of knots, dying off the bark around knots and sapwood exposure over a large area, wilting, yellowing and shedding of needles on the individual branches and whole crown may indicate the presence of pathogenic bacteria. Monitoring the health of pine plantations in Lviv and Minsk regions showed the spread of diseases that can be caused by bacteria. In order to identify the pathogenic bacteria associated with pine vascular diseases, we collected needles, bark and sapwood from symptomatic trees. Ten potential pathogenic isolates were prepared from diseased tissues. All isolates were found pathogenic and identified as Gram-positive, rod-shaped and spore-forming. The isolates were identified as Bacillus pumilus, based on morphological and biochemical characteristics, and also on 16S rRNA gene sequence analysis. Pathogenicity studies of different B. pumilus isolates revealed that they have a potential to cause the soft rot disease in pine seedlings and symptoms of wetwood disorder in young Scots pine trees.
Pinus sylvestris; bacteriosis; phytopathogenic bacteria; soft rot
Breed R.S., Murray E.G.D., Smith N.R. (1974): Bergey’s Manual of Determinative Bacteriology. Baltimore, Williams and Wilkins Company: 1268.
Cherpakov V.V. (2011): Desiccation FOREST: relationship of the organism in the pathological process. Available at http://science-bsea.narod.ru/2011/les_2011/cherpakov_us.htm (in Russian)
Galal A., El-Bana A., Janse J. (2006): Bacillus pumilus, a new pathogen on mango plants. Egyptian Journal of Phytopathology, 34: 17–29.
Grodnitskaya I.D.; Gukasyan A.B. (1999): Bacterial diseases of conifer seedlings in forest nurseries of Central Siberia. Microbiology, 68: 189–193.
Hammer O., Harper D.A.T., Ryan P.D. (2001): PAST: Paleontological statistic software package for education and data analysis. Paleontologia Eletronica, 4: 1–9.
Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. (1994): Bergey’s manual of systematic bacteriology. Baltimore, Williams and Wilkins Company: 1750.
Li B., Qiu W., Tan Q.M., Su T., Fang Y., Xie G.L. (2009): Association of a Bacillus species with leaf and twig dieback of Asian pear (Pyrus pyrifolia) in China. Journal of Plant Pathology, 91: 705–708.
Rozenfeld V.V. (2008): Epiphyte and Endophyte Microflora Scotch Pine Seeds in Kyiv Polissia Region. [Ph.D. Thesis.] Kiev, National Agricultural University of Cabinet of Ministry of Ukraine: 160.
Rybalko T., Gukasyan A. (1986): Bacteriosis in Conifer of Siberia. Novosibirsk, Nauka: 83.
Saleh O. I., Huang Pi-Yu, Huang Jeng-Sheng (1997): Bacillus pumilus, the Cause of Bacterial Blotch of Immature Balady Peach in Egypt. Journal of Phytopathology, 145, 447-453 https://doi.org/10.1111/j.1439-0434.1997.tb00348.x
Schaad N.W., Jones J.B., Chun W. (2001): Laboratory Guide for Identification of Plant Pathogenic Bacteria. St. Paul, American Phytopathological Society Press: 398.
Sczerbin-Porfinenko А.D. (1963): Bacterial diseases of forest trees. Moscow, Goslesbumizdat: 148.
Spaulding P. (1958): Disease of Foreign Forest Trees Growing in the United States. Washington, DC, U.S. Dept. of Agriculture: 118.
Urosevic B. (1968): Bark necrosis-gum flow in Spruce
P. excelsa. Lesnický časopis, 14: 307–316.
Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. (1991): 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703.
Westcott C. (2001): Westcott’s Plant Disease Handbook. London, Springer: 1008.