Ectomycorrhiza-hydrogel additive enhanced growth of Norway spruce seedlings in a nutrient-poor peat substrate

https://doi.org/10.17221/29/2022-JFSCitation:

Repáč I., Parobeková Z., Belko M. (2022): Ectomycorrhiza-hydrogel additive enhanced growth of Norway spruce seedlings in a nutrient-poor peat substrate. J. For. Sci., 68: 170–181.

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Seedling quality is an important input affecting the outplanted seedling performance. Morphological attributes and association with symbiotic ectomycorrhizal (ECM) fungi belong to influential traits determining seedling quality. In this study, the effect of pre-sowing applied commercial additives Ectovit (containing hydrogel and ECM fungi), Mycorrhizaroots (ECM fungi and nutrients) and Vetozen (natural mineral fertilizer) on the growth and ectomycorrhiza formation of Norway spruce seedlings grown in nutrient-poor pure peat in an open nursery site was assessed. Two-year-old bareroot seedlings were transplanted into containers. No significant growth differences were detected between treatments for 1 + 0 seedlings but the values of growth parameters (root collar diameter; stem height; shoot, root and total dry weight) of Ectovit-inoculated seedlings were significantly higher than those in the other treatments including the control after the second (2 + 0 seedlings) and the third growing season (2 + 1). Root-to-shoot dry weight ratio and number of root tips were distinctly higher after the third year compared to the previous two years but they were not significantly different between treatments. Mycorrhizaroots and Vetozen did not have any significant effect on seedling development. Although the occurrence of treatment-specific ECM morphotype was detected in Ectovit-inoculated seedlings, the high total ECM colonization of roots in all treatments including the control indicated a crucial impact of naturally occurring fungi on ectomycorrhiza formation.

References:
Agerer R. (2012): Color Atlas of Ectomycorrhizae (Delivery 1–15). Schwäbisch Gmünd, Einhorn Verlag: 18.
 
Agerer R., Rambold G. (2021): DEEMY – an information system for characterization and determination of Ectomycorrhizae. Available at: http://www.deemy.de
 
Aleksandrowicz-Trzcinska M., Hamera-Dzierzanowska A., Zybura H., Drozdowski S. (2013): Effect of mycorrhization and chitosan on the growth of Scots pine (Pinus sylvestris L.) in nursery and plantation. Sylwan, 157: 899–908.
 
Bošeľa M., Sedmák R., Sedmáková D., Marušák R., Kulla L. (2014): Temporal shifts of climate–growth relationships of Norway spruce as an indicator of health decline in the Beskids, Slovakia. Forest Ecology and Management, 325: 108–117. https://doi.org/10.1016/j.foreco.2014.03.055
 
Brunner I., Brodbeck S. (2001): Response of mycorrhizal Norway spruce seedlings to various nitrogen loads and sources. Environmental Pollution, 114: 223–233. https://doi.org/10.1016/S0269-7491(00)00219-0
 
Brus D.J., Hengeveld G.M., Walvoort D.J.J., Goedhart P.W., Heidema A.H., Nabuurs G.J., Gunia K. (2012): Statistical mapping of tree species over Europe. European Journal of Forest Research, 131: 145–157. https://doi.org/10.1007/s10342-011-0513-5
 
Chirino E., Villagrosa A., Vallejo R.V. (2011): Using hydrogel and clay to improve the water status of seedlings for dryland restoration. Plant and Soil, 344: 99–110. https://doi.org/10.1007/s11104-011-0730-1
 
Eltrop L., Marchner H. (1996): Growth and mineral nutrition of non-mycorrhizal and mycorrhizal Norway spruce (Picea abies) seedlings grown in semi-hydroponic sand culture. New Phytologist, 133: 469–478. https://doi.org/10.1111/j.1469-8137.1996.tb01914.x
 
Euforgen (2009): Distribution map of Norway spruce (Picea abies). Available at: http://www.euforgen.org
 
Finlay R.D. (2008): Ecological aspects of mycorrhizal symbiosis: With special emphasis on the functional diversity of interactions involving the extraradical mycelium. Journal of Experimental Botany, 59: 1115–1126. https://doi.org/10.1093/jxb/ern059
 
Gallo J., Baláš M., Linda R., Kuneš I. (2020): The effects of planting stock size and weeding on survival and growth of small-leaved lime under drought-heat stress in the Czech Republic. Austrian Journal of Forest Science, 137: 43–66.
 
Grossnickle S.C. (2012): Why seedlings survive: Influence of plant attributes. New Forests, 43: 711–738. https://doi.org/10.1007/s11056-012-9336-6
 
Gossnickle S.C., El-Kassaby Y.A. (2016): Bareroot versus container stocktypes: A performance comparison. New Forests, 47: 1–51. https://doi.org/10.1007/s11056-015-9476-6
 
Grossnickle S.C., MacDonald J.E. (2017): Why seedlings grow: Influence of plant attributes. New Forests, 49: 1–34. https://doi.org/10.1007/s11056-017-9606-4
 
Heiskanen J. (1995): Irrigation regime affects water and aeration conditions in peat growth medium and the growth of containerized Scots pine seedlings. New Forests, 9: 181–195. https://doi.org/10.1007/BF00035486
 
Hytönen J., Jylhä P. (2008): Fifteen-year response of weed control intensity and seedling type on Norway spruce survival and growth on arable land. Silva Fennica, 42: 355–368. https://doi.org/10.14214/sf.242
 
Ingleby K., Mason P.A., Last F.T., Fleming L.V. (1990): Identification of Ectomycorrhizas. London, HMSO: 112.
 
Ingleby K., Wilson J., Mason P.A., Munro R.C. (1994): Effects of mycorrhizal inoculation and fertilizer regime on emergence of Sitka spruce seedlings in bare-root nursery seedbeds. Canadian Journal of Forest Research, 24: 618–623. https://doi.org/10.1139/x94-081
 
Ivetić V., Grossnickle S., Škorić M. (2016): Forecasting the field performance of Austrian pine seedlings using morphological attributes. iForest – Biogeosciences and Forestry, 10: 99–107. https://doi.org/10.3832/ifor1722-009
 
Johansson K., Hajek J., Sjölin O., Normark E. (2015): Early performance of Pinus sylvestris and Picea abies – A comparison between seedling size, species, and geographic location of the planting site. Scandinavian Journal of Forest Research, 30: 388–400.
 
Jurásek A., Leugner J., Martincová J. (2009): Effect of initial height of seedlings on the growth of planting material of Norway spruce (Picea abies [L.] Karst.) in mountain conditions. Journal of Forest Science, 55: 112–118. https://doi.org/10.17221/97/2008-JFS
 
Korkama T., Pakkanen A., Pennanen T. (2006): Ectomycorrhizal community structure varies among Norway spruce (Picea abies) clones. New Phytologist, 171: 815–824. https://doi.org/10.1111/j.1469-8137.2006.01786.x
 
Kropp B.R., Langlois E.G. (1990): Ectomycorrhizae in reforestation. Canadian Journal of Forest Research, 20: 438–451. https://doi.org/10.1139/x90-061
 
Kwaśna H., Szewczyk W. (2016): Effects of fungi isolated from Quercus robur roots on growth of oak seedlings. Dendrobiology, 75: 99–112. https://doi.org/10.12657/denbio.075.010
 
Le Tacon F., Jung G., Mugnier J., Michelot P., Mauperin C. (1985): Efficiency in a forest nursery of an ectomycorrhizal fungus inoculum produced in a fermentor and entrapped in polymeric gels. Canadian Journal of Botany, 63: 1664–1668. https://doi.org/10.1139/b85-229
 
Le Tacon F., Bouchard D., Perrin R. (1986): Effects of soil fumigation and inoculation with pure culture of Hebeloma cylindrosporum on survival, growth and ectomycorrhizal development of Norway spruce and Douglas fir seedlings. European Journal of Forest Pathology, 16: 257–265. https://doi.org/10.1111/j.1439-0329.1986.tb00190.x
 
Lehto T. (1994): Effects of soil pH and calcium on mycorrhizas of Picea abies. Plant and Soil, 163: 69–75. https://doi.org/10.1007/BF00033942
 
Marx D.H. (1991): The practical significance of ectomycorrhizae in forest establishment. In: Ecophysiology of Ectomycorrhizae of Forest Trees (The Marcus Wallenberg Foundation Symposia Proceedings), Stockholm, Sept 27, 1991: 54–90.
 
Maltz M.R., Treseder K.K. (2015): Sources of inocula influence mycorrhizal colonization of plants in restoration projects: A meta‐analysis. Restoration Ecology, 23: 625–634. https://doi.org/10.1111/rec.12231
 
Mexal J.G., South D.B. (1991): Bareroot seedling culture. In: Duryea M.L., Dougherty P.M. (eds): Forest Regeneration Manual. Dordrecht, Kluwer Academic Publishers: 89–115.
 
Mezei P., Jakuš R., Pennerstorfer J., Havašová M., Škvarenina J., Ferenčík J., Slivinský J., Bičárová S., Bilčík D., Blaženec M., Netherer S. (2017): Storms, temperature maxima and the Eurasian spruce bark beetle Ips typographus – An infernal trio in Norway spruce forests of the Central European High Tatra Mountains. Agricultural and Forest Meteorology, 242: 85–95. https://doi.org/10.1016/j.agrformet.2017.04.004
 
Möttönen M., Lehto T., Aphalo P.J. (2001): Growth dynamics and mycorrhizas of Norway spruce (Picea abies) seedlings in relation to boron supply. Trees, 15: 319–326. https://doi.org/10.1007/s004680100106
 
Omi S.K. (1991): The target seedling and how to produce it. In: Van Buijtenen J.P., Simms T. (eds): Proceedings of Nursery Management Workshop, Alexandria, Sept 10–12, 1991: 88–118.
 
Ostonen I., Lõhmus K. (2003): Proportion of fungal mantle, cortex and stele of ectomycorrhizas in Picea abies (L.) Karst. in different soils and site conditions. Plant and Soil, 257: 435–442. https://doi.org/10.1023/A:1027305906159
 
Parobeková Z., Sedmáková D., Kucbel S., Pittner J., Jaloviar P., Saniga M., Balanda M., Vencurik J. (2016): Influence of disturbances and climate on high-mountain Norway spruce forests in the Low Tatra Mts., Slovakia. Forest Ecology and Management, 380: 128–138. https://doi.org/10.1016/j.foreco.2016.08.031
 
Pietras M., Rudawska M., Leski T., Karliński L. (2013): Diversity of ectomycorrhizal fungus assemblages on nursery grown European beech seedlings. Annals of Forest Science, 70: 115–121. https://doi.org/10.1007/s13595-012-0243-y
 
Pinto J.R., Marshall J.D., Dumroese R.K., Davis A.S., Cobos D.R. (2011): Establishment and growth of container seedlings for reforestation: A function of stocktype and edaphic conditions. Forest Ecology Management, 261: 1876–1884. https://doi.org/10.1016/j.foreco.2011.02.010
 
Read D.J., Perez-Moreno J. (2003): Mycorrhizas and nutrient cycling in ecosystems – A journey towards relevance? New Phytologist, 157: 475–492. https://doi.org/10.1046/j.1469-8137.2003.00704.x
 
Renou-Wilson F., Keane M., Farrell E.P. (2008): Efect of planting stocktype and cultivation treatment on the establishment of Norway spruce on cutaway peatlands. New Forests, 36: 307–330. https://doi.org/10.1007/s11056-008-9102-y
 
Repáč I. (1996): Inoculation of Picea abies (L.) Karst. seedlings with vegetative inocula of ectomycorrhizal fungi Suillus bovinus (L.: Fr.) O. Kuntze and Inocybe lacera (Fr.) Kumm. New Forests, 12: 41–54. https://doi.org/10.1007/BF00029981
 
Repáč I., Sendecký M. (2018): Response of juvenile Norway spruce (Picea abies [L.] Karst.) to ectomycorrhizal inoculation of perlite-peat substrates in a nursery. Journal of Sustainable Forestry, 37: 771–786. https://doi.org/10.1080/10549811.2018.1485583
 
Repáč I., Vencurik J., Balanda M. (2011): Testing of microbial additives in the rooting of Norway spruce (Picea abies [L.] Karst.) stem cuttings. Journal of Forest Science, 57: 555–564. https://doi.org/10.17221/21/2011-JFS
 
Repáč I., Balanda M., Vencurik J., Kmeť J., Krajmerová D., Paule L. (2014): Effects of substrate and ectomycorrhizal inoculation on the development of two-years-old container-grown Norway spruce (Picea abies Karst.) seedlings. iForest – Biogeosciences and Forestry, 8: 487–496. https://doi.org/10.3832/ifor1291-007
 
Repáč I., Belko M., Krajmerová D., Paule L. (2021): Planting time, stocktype and additive effects on the development of spruce and pine plantations in Western Carpathian Mts. New Forests, 52: 449–472. https://doi.org/10.1007/s11056-020-09804-3
 
Rincón A., Alvarez I.F., Pera J. (2001): Inoculation of containerized Pinus pinea L. seedlings with seven ectomycorrhizal fungi. Mycorrhiza, 11: 265–271. https://doi.org/10.1007/s005720100127
 
Rose R., Atkinson M., Gleason J., Sabin T. (1991): Root volume as a grading criterion to improve field performance of Douglas-fir seedlings. New Forests, 5: 195–209. https://doi.org/10.1007/BF00028111
 
Rossi M.J., Furigo Jr. A., Oliveira V.L. (2007): Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations. Food Technology and Biotechnology, 45: 277–286.
 
Rudawska M., Leski T. (2009): The significance of knowledge about ectomycorrhizal fungal community in bare-root nurseries for artificial inoculation. Sylwan, 153: 16–26.
 
Rudawska M., Leski T., Trocha L.K., Gornowicz R. (2006): Ectomycorrhizal status spruce seedlings from bare-root forest nurseries. Forest Ecology and Management, 236: 375–384. https://doi.org/10.1016/j.foreco.2006.09.066
 
Salcido-Ruiz S., Prieto-Ruíz J.Á., García-Rodríguez J.L., Santana-Aispuro E., Chávez-Simental J.A. (2020): Mycorrhiza and fertilization: Effect on the production of Pinus engelmannii Carr. in nursery. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 26: 327–342. https://doi.org/10.5154/r.rchscfa.2019.11.080
 
Sanchez-Zabala J., Majada J., Martín-Rodrigues N., Gonzalez-Murua C., Ortega U., Alonso-Graña M., Arana O., Duñabeitia K.M. (2013): Physiological aspects underlying the improved outplanting performance of Pinus pinaster Ait. seedlings associated with ectomycorrhizal inoculation. Mycorrhiza, 23: 627–640. https://doi.org/10.1007/s00572-013-0500-4
 
Seyfried G.S., Canham C.D., Dalling J.W., Yang W.H. (2021): The effects of tree-mycorrhizal type on soil organic matter properties from neighborhood to watershed scales. Soil Biology and Biochemistry, 161: 108385. https://doi.org/10.1016/j.soilbio.2021.108385
 
Siddiqui Z.A., Kataoka R. (2011): Mycorrhizal inoculants: Progress in inoculant production technology. In: Ahmad I., Ahmad F., Pichtel J. (eds): Microbes and Microbial Technology. New York, Springer: 489–506.
 
Sierota Z. (2019): Is the introduction of covered root seedlings in every renewal reasonable – Phytopathological point of view. Sylwan, 163: 989–996.
 
Sliwa S. (2009): Ten years of experience with controlled mycorrhization of forest tree seedlings grown in the Rudy Raciborskie container nursery. Sylwan, 153: 260–265.
 
Smaill S.J., Walbert K. (2013): Fertilizer and fungicide use increases the abundance of less beneficial ectomycorrhizal species in a seedling nursery. Applied Soil Ecology, 65: 60–64. https://doi.org/10.1016/j.apsoil.2013.01.007
 
Tahat M.M., Kamaruzaman S., Othman R. (2010): Mycorrhizal fungi as a biocontrol agent. Plant Pathology Journal, 9: 198–207. https://doi.org/10.3923/ppj.2010.198.207
 
Trakal L., Neuberg M., Száková J., Vohník M., Tejnecký V., Drábek O., Tlustoš P. (2013): Phytoextraction and assisted phytoextraction of metals from agriculture used soil. Communication in Soil Science and Plant Analysis, 44: 1862–1872. https://doi.org/10.1080/00103624.2013.790403
 
Vodnik D., Gogala N. (1994): Seasonal fluctuations of photosynthesis and its pigments in 1-year mycorrhized spruce seedlings. Mycorrhiza, 4: 277–281. https://doi.org/10.1007/BF00206777
 
Vuorinen I., Hamberg L., Müller M., Seiskari P., Pennanen T. (2015): Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce (Picea abies) seedlings. Mycorrhiza, 25: 311–324. https://doi.org/10.1007/s00572-014-0611-6
 
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