Identification and genetic diversity of Acer ibericum (Aceraceae) in South Caucasus

https://doi.org/10.17221/36/2021-JFSCitation:

Paule J., Goginashvili N., Tvauri I., Paule L., Gabashvili E., Kotetishvili M., Kobakhidze N. (2021): Identification and genetic diversity of Acer ibericum (Aceraceae) in South Caucasus. J. For. Sci., 67: 420–426.

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Internal transcribed spacer (ITS) sequence was tested in Acer ibericum for its suitability as a barcoding as well as population genetic marker. The marker was reliably used to identify A. ibericum as well as to gain preliminary insights into the genetic diversity of Georgian populations. MegaBLAST searches of studied samples against the GenBank database revealed that all studied accessions could be considered Acer ibericum. Populations from Vashlovani National Park revealed the presence of unique ribotypes, and together with the population from Shavi Mountain, they were identified as the most genetically diverse ones. Based on presented data the populations from Eastern Georgia should be prioritized if conservation measures are planned.

References:
Akhalkatsi M (2010): Habitats of Georgia. Tbilisi, Natura 2000: 118.
 
Calonje M., Martín-Bravo S., Dobeš C., Gong W., Jordon-Thaden I., Kiefer C., Kiefer M., Paule J., Schmickl R., Koch M.A. (2009): Non-coding nuclear DNA markers in phylogenetic reconstruction. Plant Systematics and Evolution, 282: 257–280. https://doi.org/10.1007/s00606-008-0031-1
 
Chen S., Yao H., Han J., Liu C., Song J., Shi L., Zhu Y., Ma X., Gao T., Pang X., Luo K., Li Y., Li X., Jia X., Lin Y., Leon C. (2010): Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One, 5: e8613. https://doi.org/10.1371/journal.pone.0008613
 
Clement M., Snell Q., Walke P., Posada D., Crandall K. (2002): TCS: Estimating gene genealogies. In: Proceedings 16th International Parallel and Distributed Processing Symposium (IPDPS 2002), Fort Lauderdale, Apr 15–19, 2001: 184.
 
de Jong P.C. (1994): Taxonomy and reproductive biology of maples. In: van Gelderen D.M., de Jong P.C., Oterdoom H.J. (eds): Maples of the World. Portland, Timber Press: 69–103.
 
Dolukhanov A. (1948): Two maples new for the Caucasus. Bulletin of Georgian Academy of Sciences, 9: 603–605. (in Georgian)
 
Fazekas A.J., Kesanakurti P.R., Burgess K.S., Percy D.M., Graham S.W., Barrett S.C.H., Newmaster S.G., Hajibabaei M., Husband B.C. (2009): Are plant species inherently harder to discriminate than animal species using DNA barcoding markers? Molecular Ecology Resources, 9: 130–139. https://doi.org/10.1111/j.1755-0998.2009.02652.x
 
Grimm G.W., Denk T. (2014): The Colchic region as refuge for relict tree lineages: Cryptic speciation in field maples. Turkish Journal of Botany, 38: 1050–1066. https://doi.org/10.3906/bot-1403-87
 
Grimm G.W., Renner S.S., Stamatakis A., Hemleben V. (2006): A nuclear ribosomal DNA phylogeny of Acer inferred with maximum likelihood, splits graphs, and motif analysis of 606 sequences. Evolutionary Bioinformatics Online, 2: 7–22.
 
Grimm G.W., Denk T., Hemleben V. (2007): Evolutionary history and systematics of Acer section Acer – a case study of low-level phylogenetics. Plant Systematics and Evolution, 267: 215–253. https://doi.org/10.1007/s00606-007-0572-8
 
Han Y.-W., Duan D., Ma X.-F., Jia Y., Liu Z.-L., Zhao G.-F., Li Z.-H. (2016): Efficient identification of the forest tree species in Aceraceae using DNA barcodes. Frontiers in Plant Science, 7: 1707.
 
Hebert P.D.N., Cywinska A., Ball S.L., deWaard J.R. (2003): Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270: 313–321. https://doi.org/10.1098/rspb.2002.2218
 
Johnson L.P.V. (1939): A descriptive list of natural and artificial interspecific hybrids in North American forest-tree genera. Canadian Journal of Research, 17: 411–444. https://doi.org/10.1139/cjr39c-040
 
Katoh K., Standley D.M. (2013): MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30: 772–780. https://doi.org/10.1093/molbev/mst010
 
Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., Buxton S., Cooper A., Markowitz S., Duran C., Thierer T., Ashton B., Meintjes P., Drummond A. (2012): Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28: 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
 
Khademi H., Mehregan I., Assadi M., Nejadsatari T., Zarre S. (2016): Molecular phylogeny of Acer monspessulanum L. subspecies from Iran inferred using the ITS region of nuclear ribosomal DNA. Biodiversitas Journal of Biological Diversity, 17: 16–23.
 
Lachashvili J., Lachashvili N., Khachidze M. (2007): Conspectus of Flora of Kiziki (Eastern Georgia). Tbilisi, Universal: 375. (in Georgian)
 
Leigh J.W., Bryant D. (2015): POPART: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6: 1110–1116. https://doi.org/10.1111/2041-210X.12410
 
Librado P., Rozas J. (2009): DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinfomatics, 25: 1451–1452. https://doi.org/10.1093/bioinformatics/btp187
 
Marschall von Bieberstein F.A. (1808): Flora Taurico-Caucasica exhibens stirpes phaenogamas, in Chersoneso Taurica et regionibus caucasicis sponte crescentes. Charkovia: 478. (in Latin)
 
Nei M. (1987): Molecular Evolutionary Genetics. New York, Columbia University Press: 512.
 
Nei M., Tajima F. (1983): Maximum likelihood estimation of the number of nucleotide substitutions for restriction sites data. Genetics, 105: 207–217. https://doi.org/10.1093/genetics/105.1.207
 
Pojárkova A.J. (1949): Aceraceae Lindl. In: Komarov V.A. (ed.): Flora SSSR 14. Moskva, Leningrad, Izd. Akademii Nauk SSSR: 580-622. (in Russian)
 
Ratnasingham S., Hebert P.D.N. (2013): A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. PLoS One, 8: e66213. https://doi.org/10.1371/journal.pone.0066213
 
Tarkhnishvili D., Chaladze G. (eds) (2013): Georgian biodiversity database. Available at: http://www.biodiversity-georgia.net (Accessed 7 December 2020).
 
van Gelderen D.M., de Jong P.C., Oterdoom H.J., van Hoey Smith J.R.P., Dudley T.R. (1994): Maples of the World. Portland, Timber Press: 458.
 
WFO (2020): World Flora Online. Available at: http://www.worldfloraonline.org (Accessed 7 December 2020).
 
Yaltirik F. (1967a): Contribution to the taxonomy of woody plants in Turkey. Notes from the Royal Botanical Garden Edinburgh, 28: 9–10.
 
Yaltirik F. (1965b): Acer L. In: Davis P.H., Cullen J., Coode M.J.E. (eds): Flora of Turkey and the East Aegean Islands. Volume 2. Edinburgh, Edinburgh University Press: 509–519.
 
Zhang Z., Schwartz S., Wagner L., Miller W. (2000): A greedy algorithm for aligning DNA sequences. Journal of Computational Biology, 7: 203– 214. https://doi.org/10.1089/10665270050081478
 
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