The effect of pollen performance on low seed fertility in a Greek population of Juniperus excelsa E., Varsamis G., Koutseri I., Takos I., Merou T. (2019): The effect of pollen performance on low seed fertility in a Greek population of Juniperus excelsa. J. For. Sci., 65: 356-367.
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The Juniperus excelsa (Cupressaceae) forest in northern Greece has been facing a documented severe decline of natural regeneration over the last few years. In order to dissect the physiological components of the low regeneration, pollen originated from different sites as well as from trees of different height was investigated in terms of viability and in vitro germination capacity. In addition, embryo development and viability of the first year immature seeds were further assessed. Pollen was found to present high viability (61.5–93.2%) in all sites and tree height classes. However, pollen germination was quite low (maximum germination percentage recorded was 30.2%). The low pollen germination capacity recorded in trees above 6 m in height was reflected in the subsequent low percentages of successful embryo development and viability found in the produced seeds. These results suggest that low seed fertility in Juniperus excelsa is associated with reduced pollen germination, suggesting a complex regeneration mechanism and is considered an additional step in shedding light on the physiological basis of the low natural regeneration of Juniperus excelsa.

Boavida L.C., McCormick S. (2007): Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana. The Plant Journal, 52: 570–582.
Brewbaker J.L., Kwack B.H. (1963): The essential role of calcium ion in pollen germination and pollen tube growth. American Journal of Botany, 50: 859–865.
Chen Y.F., Matsubayashi Y., Sakagami Y. (2000): Peptide growth factor phytosulfokine-α contributes to the pollen population effect. Planta, 211: 752.
Danti R., Della Rocca G., Calamassi R., Mori B., Mariotti Lippi M. (2011): Insights into a hydration regulating system in Cupressus pollen grains. Annals of Botany, 108: 299–306.
Douaihy B., Vendramin G.G., Boratyński A., Machon N., BouDagher-Kharrat M. (2011): High genetic diversity with moderate differentiation in Juniperusexcelsa from Lebanon and the eastern Mediterranean region. AoB Plants, 2011.doi:10.1093/aobpla/plr003
Dumont-BéBoux N., Weber M., Ma Y., vonAderkas P. (1998): Intergeneric pollen-megagametophyte relationships of conifers in vitro. Theoretical and Applied Genetics, 97: 881–887.
Fernando D.D., Owens J.N., von Aderkas P., Takaso T. (1997):In vitro pollen tube growth and penetration of female gametophyte in Douglas fir (Pseudotsugamenziesii). Sexual Plant Reproduction, 10: 209–216.
Fernando D.D., Owens J.N., von Aderkas P. (1998): In vitro fertilization from co-cultured pollen tubes and female gametophytes of Douglas fir (Pseudotsugamenziesii). Theoritical and Applied Genetics,96: 1057–1063.
Gaaliche B., Majdoub A., Trad M., Mars M. (2013): Assessment of Pollen Viability, Germination, and Tube Growth in Eight Tunisian Caprifig (FicuscaricaL.) Cultivars. ISRN Agronomy, vol. 2013, Article ID 207434.
Garcia D., Zamora R., Hodar J.A., Gomez J.M. (1999): Age structure of Juniperuscommunis L. in the Iberian peninsula: Conservation of remnant populations in Mediterranean mountains. Biological Conservation, 87: 215–220.
Garcia D., Zamora R., Gomez J.M., Jordano P., Hodar J.A. (2000): Geographical variation in seed production, predation and abortion in Juniperuscommunis throughout its range in Europe. Journal of Ecology, 88: 436–446.
Gruwez R., Leroux O., De Frenne P., Tack W., Viane R., Verheyen K. (2013): Critical phases in the seed development of common juniper (Juniperuscommunis). Plant Biology, 15: 210–219.
Gruwez R., De Frenne P., De Schrijver A., Leroux O., Vangansbeke P., Verheyen K. (2014): Negative effects of temperature and atmospheric depositions on the seed viability of common juniper (Juniperuscommunis). Annals of Botany, 113: 489–500.
Gruwez R., De Frenne P., Vander Mijnsbrugge K., Vangansbeke P., Verheyen K. (2016): Increased temperatures negatively affect Juniperuscommunis seeds: evidence from transplant experiments along a latitudinal gradient. Plant Biology, 18: 417–422.
ISTA (1999): International Rules for Seed Testing. Seed Science and Technology, 27, Supplement.
Karapatzak E.K., Wagstaffe A., Hadley P., Battey N.H. (2012): High temperature-induced reductions in cropping in Everbearing Strawberries (Fragaria x ananassaDuch.) are associated with Reduced Pollen Performance. Annals of Applied Biology, 161: 255–265.
Kärkkäinen K., Savolainen O., Koski V. (1999): Why do plants abort so many developing seeds: bad offspring or bad maternal genotypes? Evolutionary Ecology, 13: 305–317.
Mead R., Curnow R.N., Hasted A.M. (2003): Statistical methods in Agriculture and experimental Biology, 3rd ed. Chapter 14. Chapman& Hall/CRC.
Meloni M., Perini D., Filigheddu R., Binelli G. (2006): Genetic variation in five Mediterranean populations of Juniperusphoenicea as revealed by Inter-Simple Sequence Repeat (ISSR) markers. Annals of Botany, 97: 299–304.
Nepi M., Guarnieri M., Mugnaini S., Cresti L., Pacini E., Piotto B. (2005): A modified FCR test to evaluate pollen viability in Juniperuscommunis L. Grana, 44: 148–151.
Owens J.N., Molder M. (1977): Sexual reproduction of Abiesamabilis. Canadian Journal of Botany, 55: 2653–2667.
Owens J.N., Molder M. (1980): Sexual reproduction of Sitcaspurce (Piceasitchensis). Canadian Journal of Botany, 58: 886–901.
Pardi M.L., Viegi L., Renzoni G.C., Franchi G.G., Pacini E. (1996): Effects of acidity on the insoluble polysaccharide content of germinating pollen of Pinus pinea L. and Pinus pinaster Aiton. Grana, 35: 240–247.
Pasonen, H. L., KäpyläM. (1998): Pollen–pollen interactions in Betula pendula in vitro. New Phytologist, 138: 481–487.
Pinna M.S., Mattana E., Cańadas E.M., Bacchetta G. (2014): Effects of pre-treatments and temperature on seed viability and germination of Juniperusmacrocarpa Sm. ComptesRendusBiologies, 337: 338–44.
Porch T.G., Jahn M. (2001): Effects of high-temperature stress on microsporogenesis in heat-sensitive and heat-tolerant genotypes of Phaseolus vulgaris. Plant, Cell and Environment, 24: 723–731.
Shivanna K.R., Rangaswamy N.S. (1992): Pollen Biology. A laboratory manual. Berlin, Heidelberg, Sringer-Verlag: 119.
Southworth D. (1988): Isolation of exines from gymnosperm pollen. American Journal of Botany, 75: 15–21.
Stampoulidis A., Milios E., Kitikidou K. (2013). The regeneration of pure Juniperus excelsa M. Bieb. stands in Prespa National Park in Greece. Sumarski List, 137: 163–172.
Strid A., Tan K. (1997): Flora Hellenica. Nordic Journal of Botany, 18: 334.
Vanden-Broeck A., Gruwez R., Cox K., Adriaenssens S., Michalczyk I.M., Verheyen K. (2011): Genetic structure and seed-mediated dispersal rates of an endangered shrub in a fragmented landscape: a case study for Juniperuscommunis in northwestern Europe. BMC Genetics, 12: 73.
Verheyen K., Adriaenssens S., Gruwez R., Michalczyk I. M.,Ward L. K.,Rosseel Y., Van den Broeck A., GarcíaD. (2009):Juniperuscommunis: victim of the combined action of climate warming and nitrogen deposition? Plant Biology, 11: 49–59.
Vrahnakis M., Fotiadis G., Kazoglou Y. (2011): Record, assessment and geographical representation of the rangeland and forest habitat types of the Natura 2000 Sites of Prespa National Park (EthnikosDrymosPrespon - GR1340001, and Mt. Varnous (Ori Varnounta - GR 1340003)) and adjacent areas - Final Report. TEI of Larissa, Society for the Protection of Prespa, 107p. (+ Annexes) (in Greek).
Ward L.K. (2010): Variation in ripening years of seed cones of Juniperuscommunis L. Watsonia, 28: 11–19.
Williams C.G., Auckland L.D., Reynolds M.M., Leach K.A. (2003):Overdominantlethals as part of the conifer embryo lethal system. Heredity, 91: 584–592.
Williams C.G. (2009): Conifer reproductive biology. Springer.
WorldClim (2019). Global climate data for ecological modeling and GIS. Available at: Accessed on 22 Sept 2018.
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