Cryopreservation of Pistacia vera embryonic axes B., Daguin F., Kaïd-Harche M. (2015): Cryopreservation of Pistacia vera embryonic axes. J. For. Sci., 61: 182-187.
download PDF
This preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually decreased from 100% to 31% after 30-month storage of seeds. Cryopreservation may thus be necessary for the long-term conservation of embryos. A simple protocol was set up using embryonic axes. It included a single dehydration step with silica gel prior to direct freezing in liquid nitrogen (–196°C). The optimal germination rate was obtained after 60 min dehydration (water content of 0.2 grams of water per gram of dry weight [g·g–1 DW]). However, 90 minutes of dehydration (0.14 g·g–1 DW) were necessary to obtain seedlings whose qualitative development was equivalent to that of the control embryonic axes.
Bani-Aameur F., Alouani M. (1999): Viabilité et dormance des semences d’arganier (Argania spinosa (L.) Skeels). Ecologia Mediterranea, 25: 75–86.
Bationo Babou Andr�, Ouedraogo Sibiri Jean, Guinko Sita (2001): Long�vit� des graines et contraintes � la survie des plantules d' Afzelia africana Sm. dans une savane bois�e du Burkina Faso. Annals of Forest Science, 58, 69-75
Beardmore Tannis, Vong Wendy (1998): Role of the cotyledonary tissue in improving low and ultralow temperature tolerance of butternut ( Juglanscinerea ) embryonic axes. Canadian Journal of Forest Research, 28, 903-910
Benmahioul Benamar, Kaid-Harche Meriem, Dorion Noëlle, Daguin Florence (2009): In vitro embryo germination and proliferation of pistachio (Pistacia vera L.). Scientia Horticulturae, 122, 479-483
Berjak P., Dumet D. (1996): Cryopreservation of seeds and isolated embryonic axes of neem (Azadirachta indica). CryoLetters, 17: 99–104.
Daws Matthew I., Davies Jennifer, Vaes Elly, van Gelder Roelof, Pritchard Hugh W. (2007): Two-hundred-year seed survival of Leucospermum and two other woody species from the Cape Floristic region, South Africa. Seed Science Research, 17, 73-
De Boucaud M.T., Brison M., Ledoux C., Germain E. (1991): Cryopreservation of embryonic axes of recalcitrant seed: Juglans regia L. cv Franquette. CryoLetters, 12: 163–166.
Engelmann F. (2004): Plant cryopreservation: progress and prospects. In Vitro Cellular & Developmental Biology-Plant, 40: 427–433.
Gonzalez-Benito M.E., Prieto R.M., Herradon E., Martin C. (2002): Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors. CryoLetters, 23: 283–290.
Jacquy P. (1972): Multiplication du pistachier en pépinière. Projet d’expérimentation et de démonstration sur certaines productions fruitières, fourragères et animales. Rome, FAO: 49.
Joley L.E. (1979): Pistachio. In: Jaynes R.A. (ed.): Nut tree culture in North America. Hamden, Northern Nut Growers Association: 163–174.
Li De-Zhu, Pritchard Hugh W. (2009): The science and economics of ex situ plant conservation. Trends in Plant Science, 14, 614-621
Makeen M.A., Noor N.M., Dussert S., Clyde M.M. (2005): Cryopreservation of whole seeds and excised embryonic axes of Citrus suhuiensis cv. Limau langkat in accordance to their desiccation sensitivity. CryoLetters, 26: 259–268.
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497
Nagel Manuela, Börner Andreas (2010): The longevity of crop seeds stored under ambient conditions. Seed Science Research, 20, 1-
Ozden-Tokatli Y., Ozudogru E.A., Gumusel F., Lambardi M. (2007): Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing. CryoLetters, 28: 83–94.
Ozden-Tokatli Y., Akdemir H., Tilkat E., Onay A. (2010): Current status and conservation of Pistacia germplasm. Biotechnology Advances, 28, 130-141
Piotto B., Di Noi A. (2003): Seed propagation of Mediterranean trees and shrubs. Rome, APAT – Agency for the Protection of the Environment and for Technical Services: 108.
Pence Valerie C. (1990): Cryostorage of embryo axes of several large-seeded temperate tree species. Cryobiology, 27, 212-218
Walters C., Toucell D.H., Power P., Wesley-Smith J., Antonlin M.F. (2002): A cryopreservation protocol for embryos of the endangered species Zizania texana. CryoLetters, 23: 291–298.
Wesley-Smith James, Walters Christina, Pammenter N.W., Berjak Patricia (2001): Interactions among Water Content, Rapid (Nonequilibrium) Cooling to −196°C, and Survival of Embryonic Axes of Aesculus hippocastanum L. Seeds. Cryobiology, 42, 196-206
Zohary M. (1952): A monographical study of the genus Pistacia. Palestine Journal of Botany Jerusalem Series, 5: 187–228.
download PDF

© 2020 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti