Effect of different pre-treatments on seed germination of Prosopis juliflora and Dalbergia sissoo: a step towards mutation breeding

https://doi.org/10.17221/64/2019-JFSCitation:Javed Muhammad A. (2020): Effect of different pre-treatments on seed germination of Prosopis juliflora and Dalbergia sissoo: a step towards mutation breeding. J. For. Sci., 66: 80-87.
download PDF

For improving the seed germination of Prosopis juliflora and Dalbergia sissoo different treatments were tested, including side cutting, abrasion, overnight soaking in boiling water, scalding in actively boiling water for
1 minute and immersion in 30%, 60% and 95% H2SO4 solution. Results showed that abrasion with sandpaper and side cutting were the most effective methods to break seed dormancy in both species, while scalding in actively boiling water for 1 minute, overnight soaking and different concentrations of H2SO4 gave low to zero seed germination. Based on the positive effects of scarification it was concluded that seed dormancy in both species was due to water impermeability of the seed coat. Mutation breeding involves the treatment of large quantities of seeds, therefore abrasion with sandpaper was the most efficient and less labour-intensive method; this method was subsequently used for determination of LD50 as it is a prerequisite in a mutation breeding program. Abrasion before irradiation had a positive effect on P. juliflora whereas it had a negative effect on D. sissoo seeds. Seeds of both species were exposed to different doses of gamma rays such as 0, 100, 200, 300, 400 and 500 Gy using a 60Co source. The LD50 for P. juliflora was 651 Gy based on the rate of seed germination indicating that P. juliflora had tolerance to irradiation and low radiosensitivity to gamma ray. A high LD50 of 1097 Gy was observed for D. sissoo, suggesting high tolerance to irradiation and very low radio sensitivity. These findings will help to initiate a mutation breeding program in both species to obtain desirable mutants with desirable characteristics such as thornless genotypes, better tree form, disease resistance and increased genetic diversity.

Asif M.J., Mak C., Othman R. (2001): In vitro zygotic embryo culture of wild Musa acuminata ssp. malaccensis and factors affecting germination and seedling growth. Plant Cell Tissue and Organ Culture, 67: 267–270. https://doi.org/10.1023/A:1012781531641
Benech R.L., Giallorenzi M., Frank J., Rodriguez V. (1998): Termination of hull-imposed dormancy in developing barley grains is correlated with changes in embryonic ABA levels and sensitivity. Seed Science Research, 9: 39–47. https://doi.org/10.1017/S0960258599000045
Bewley J.D. (1997): Seed germination and dormancy. Plant Cell, 9: 1055–1066. https://doi.org/10.1105/tpc.9.7.1055
Black M., Bewley J.D. (2000): Seed technology and its biological basis. Sheffield, CRC Press: 419.
Chan Y.K. (2009): Radiation-induced mutation breeding of papaya. In: Jain M.S., Spencer M.M. (eds.): Induced mutation in tropical fruit trees. IAEA Technical document, 1615. Austria, International Atomic Energy Agency: 170.
Cox C.G., Munger H.M., Smith E.A. (1945): A germination inhibitor in the seed coats of certain varieties of cabbage. Plant Physiology, 20: 289–294. https://doi.org/10.1104/pp.20.2.289
Fedrico P. O., Mollard P. (2009): Breaking Setaria parviflora seed dormancy by nitrates and light is part of a mechanism that detects a draw down period after flooding. Aquatic Botany, 91: 54–60.
Government of Pakistan (1991): The Pakistan National ConServation Strategy: where we are, where we should be, and how to get there. Karachi, The World Conservation Union, IUCN: 396.
Kangarasu S., Ganeshram S., Joel A.J. (2014): Determination of lethal dose for gamma rays and ethyl methane sulphonate induced mutagenesis in cassava (Manihot esculenta Crantz.). International Journal of Scientific Research, 3: 3–6.
Kiong A.L.P., Lai A.G., Hussein S., Harun A.R. (2008): Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. American-Eurasian Journal of Sustainable Agriculture, 2: 135–149.
Kon E., Ahmed O.H., Samin S., Majid N.M.A. (2007): Gamma radio-sensitivity study on long bean (Vigna esquipedalis). American Journal of Applied Sciences, 4: 1090–1093. https://doi.org/10.3844/ajassp.2007.1090.1093
Kumar D.P., Chaturvedi A., Sreedhar M., Aparna M., Venu-Babu P., Singhal R.K. (2013): Gamma radio sensitivity study on rice (Oryza sativa L.). Asian Journal of Plant Science & Research, 3: 54–68.
Kovacs E., Keresztes A. (2002): Effect of gamma and UV-B/C radiation on plant cells. Micron, 33: 199–210. https://doi.org/10.1016/S0968-4328(01)00012-9
Koorneef M.L., Bentsink H.H. (2002): Seed dormancy and germination. Plant Biology, 5: 33–36.
Mares D.J. (2005): Quarterly reports on plant growth regulation and activities of the PGRSA. Plant Growth Regulation Society of America, 33: 78–89.
Mahmood B., Shanta I.A.E., Hisham N.A. (2015): Germination responses of Acacia cyclops and Acacia victoriae seeds to different scarification treatments. In: 3rd International Conference on Biological, Chemical & Environmental Sciences (BCES-2015), Kuala Lumpur, Sept 21–22: 119–124.
Mawahib A., EL-Khalifa A. (2004): Effect of seed scarification methods on germination and seed vigour of poinciana (Delonix regia). [M.Sc (Hons.) Thesis.] Khartoum, University of Khartoum: 62.
Pasiecznik N.M., Harris P.J.C., Tavares J.P., Cassama M. (1998): Pre-treatment of Prosopis seeds to break dormancy. International Tree Crops Journal, 9: 187–193. https://doi.org/10.1080/01435698.1998.9752974
Quraishi M.A.A. (2012): Basics of Forestry and Allied sciences, Vol. 1. Faisalabad, University of Agriculture: 310.
R Core Team (2018): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Rajput N.A., Pathan M.A., Rajput A.Q., Jiskani M.M., Lodhi A.M., Rajput S.A., Khaskhali M.I. (2010): Isolation of fungi associated with Shisham trees and their effect on seed germination and seedling mortality. Pakistan Journal of Botany, 42: 369–374.
Rincon-Rosales R., Culbero-Espinosa N.R., Gutierrez-Miceli F.A., Dedoren l. (2003): Scarification of seeds of Acacia angustissima and its effect on germination. Seed Science and Technology, 31: 301–307. https://doi.org/10.15258/sst.2003.31.2.07
Songsri P., Suriharn B., Sanitchon J., Srisawangwong S., Kesmala T. (2011): Effects of gamma radiation on germination and growth characteristics of physic nut (Jatropha curcas L.). Journal of Biological Sciences, 11: 268–274. https://doi.org/10.3923/jbs.2011.268.274
Soyler D., Khawar K.M. (2006): Effects of pre-chilling, scarification, incubation temperature, photoperiod, KNO3 and GA3 treatments on germination of caper (Capparis Ovata Desf. Var. Palaestina Zoh.) seeds. Propagation of Ornamental Plants, 6: 159–164.
Uzen F., Aydin I. (2004): Improving germination rate of Medicago and Trifolium species. Asian Journal of Plant Science, 6: 714–717.
Vanstone D. E. (1978): Basswood (Tilia americansl) seed germination. Combined Proceedings of the International Plant Propagatols Society, 28: 566–570.
Zaman S. B., Ahmad S. (2009): Salinity and waterlogging in the Indus basin of Pakistan: Economic loss to agricultural economy. A series of NRD Research Briefing, PARC, Islamabad: 21–26.
Zare S., Tavili A. M., Darini J. (2011): Effects of different treatments on seed germination and breaking seed dormancy of Prosopis koelziana and Prosopis juliflora. Journal of Forestry Research, 22: 35–38. https://doi.org/10.1007/s11676-011-0121-8
download PDF

© 2020 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti