Root rot and damping-off of Aleppo pine seedlings caused by Pythium spp. in Algerian forest nurseries F., Belabid L., Sánchez J., Gallego E. (2016): Root rot and damping-off of Aleppo pine seedlings caused by Pythium spp. in Algerian forest nurseries. J. For. Sci., 62: 322-328.
download PDF
Aleppo pine (Pinus halepensis Miller) is a common forest tree in the Mediterranean Region. Production of Aleppo pine seedlings is hindered by damping off and root rot diseases in cooler conditions of north-western Algeria, which significantly reduces the seedling emergence. This work was carried out to evaluate the pathogenicity of different Pythium Pringsheim species on Aleppo pine seedlings. Seventeen isolates of Pythium spp. were isolated from diseased seedlings, identified to the species level based on DNA sequence from the internal transcribed spacer region and their identity was confirmed on morphological basis. The obtained isolates were pathologically characterized in Petri dish and pot assays. Germination was significantly inhibited by the different Pythium spp. isolates. The highest inhibition was observed with Pythium ultimum Trow in Petri dish and pot assays. The reduction in root growth differed significantly between different isolates. The maximum reduction in root growth (92.2%) was observed for P. ultimum in Petri dish assay. The various isolates of Pythium showed a significant influence on root length, shoot length and vigour index. The maximum disease incidence (89.7%) was caused by P. ultimum. Our results indicated that Pythium spp. is commonly found on diseased seedlings, in most containers and bare-root nurseries.
Broders K.D. (2008): Seed and seedling disease of corn and soybean in Ohio: The role of Fusarium graminearum, Pythium species diversity, fungicide sensitivity, Pythium community composition, and soil properties in disease severity. [Ph.D. Thesis.]. Columbus, Ohio State University: 204.
Dick M.W. (1990): Keys to Pythium. Reading, University of Reading: 64.
James R.L. (2012): Damping-off. In: Cram M.M., Frank M.S., Mallams K.M. (eds): Forest Nursery Pests. Agriculture Handbook No. 680. Washington, DC, USDA Forest Service: 115–116.
Kageyama Koji (2014): Molecular taxonomy and its application to ecological studies of Pythium species. Journal of General Plant Pathology, 80, 314-326
Kirkpatrick M. T., Rothrock C. S., Rupe J. C., Gbur E. E. (2006): The Effect of Pythium ultimum and Soil Flooding on Two Soybean Cultivars. Plant Disease, 90, 597-602
Lazreg F., Belabid L., Sanchez J., Gallego E., Bayaa B. (2014): Pathogenicity of Fusarium spp. associated with diseases of Aleppo pine seedlings in Algerian forest nurseries. Journal of Forest Science, 60: 115–120.
Lazreg F., Belabid L., Sanchez J., Gallego E., Garrido-Cardenas J. A., Elhaitoum A. (2013): First Report of Globisporangium ultimum Causing Pythium Damping-Off on Aleppo Pine in Algeria, Africa, and the Mediterranean Region. Plant Disease, 97, 1111-1111
Maisuria K.M., Patel S.T. (2009): Seed germinability, root and shoot length and vigor index of soybean as influenced by rhizosphere fungi. Journal of Agricultural Sciences, 22: 1120–1122.
Menzies J G, Ehret D L, Koch C, Hall J W, Seifert K A, Bissett J, Barr D JS (2005): Fungi associated with roots of cucumber grown in different greenhouse root substrates. Canadian Journal of Botany, 83, 80-92
Mezali M. (2003): Rapport sur le secteur forestier en Algérie. Available at:
Pahlavani S., Razavi E., Kavusi F., Hasanpoor M. (2009): Influence of temperature and genotype on Pythium damping-off in safflower. Journal of Plant Breeding and Crop Science, 1: 1–7.
Pankhurst C (1995): Influence of tillage and crop rotation on the epidemiology of Pythium infections of wheat in a red-brown earth of South Australia. Soil Biology and Biochemistry, 27, 1065-1073
Plaats-Niterink A., Vander J. (1981): Monograph on the Genus Pythium. Studies in Mycology. Baarn, Centraalbureau voor Schimmelcultures: 242.
Quezel P. (1986): Les pins du groupe «Halepensis»: Ecologie, végétation, écophysiologie. Options Méditerranéennes, Series B, 86: 11–25.
Ravishankar R.V., Mamatha T. (2005): Seedling diseases of some important forest trees and their management. In: Sutherland J.R., Procházková Z. (eds): Proceedings of IUFRO Meeting on Diseases and Insects in Forest Nurseries, Uherské Hradiště, Sept 10–15, 2005: 51–63.
Rosso M. Luciana, Rupe John C., Chen Pengyin, Mozzoni Leandro A. (2008): Inheritance and Genetic Mapping of Resistance to Damping-Off Caused by in ‘Archer’ Soybean. Crop Science, 48, 2215-
Song Weitang, Zhou Ligang, Yang Chengzong, Cao Xiaodong, Zhang Liqun, Liu Xili (2004): Tomato Fusarium wilt and its chemical control strategies in a hydroponic system. Crop Protection, 23, 243-247
Sutherland J., Dennis J. (1992): Pythium water mould in British Columbia forest nurseries. Seed Seedling Extension Topics, 5: 9–10.
Teymoori S., Shahri M.H., Rahnama K., Afzali H. (2012): Identification and pathogenicity of Pythium species on Cantaloupe in Khorasan Razavi province of Iran. Journal of Crop Protection, 1: 239–247.
Weber Roland W. S., Sulzer Frank-Ludger, Haarhaus Michael (2004): Pythium undulatum, cause of root rot of Abies procera Christmas trees and Pseudotsuga menziesii in Northern Germany. Mycological Progress, 3, 179-188
Weiland J.E. (2014): Understanding the pathology of Douglas-fir seedlings in Pacific Northwest nurseries. In: Wilkinson K.M., Haase D.L., Pinto J.R. (eds): RMRS-P-72: National Proceedings: Forest and Conservation Nursery Associations, Lafayette, July 22–25, 2013: 38–40.
Weiland Jerry E., Beck Bryan R., Davis Anne (2013): Pathogenicity and Virulence of Pythium Species Obtained from Forest Nursery Soils on Douglas-Fir Seedlings. Plant Disease, 97, 744-748
Zhang B. Q., Yang X. B. (2000): Pathogenicity of Pythium Populations from Corn-Soybean Rotation Fields. Plant Disease, 84, 94-99
download PDF

© 2020 Czech Academy of Agricultural Sciences