Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites

https://doi.org/10.17221/88/2016-JFSCitation:Cvrčková H., Máchová P., Poláková L., Trčková O. (2017): Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites. J. For. Sci., 63: 53-61.
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Fagus sylvatica Linnaeus (European beech), the ecologically and economically most important broadleaved tree species in the Czech Republic, was strongly reduced in the past. Today there are efforts to increase the proportion of beech to ensure optimal forest tree species composition. When extensively reintroducing beech, it is important to acquire more detailed knowledge of genetic diversity. Thirteen important beech populations in different stands in the territory of the Czech Republic were genotyped using 12 polymorphic nuclear microsatellite markers. The genotypic data from adult trees imply genetic differences between the populations. The estimated genetic diversity expressed as Shannon’s information index ranged from 1.73 to 1.92. Thirteen beech populations showed excess homozygotes, as indicated by positive fixation index (F) values (F = 0.005–0.115). The pairwise FST values indicated low genetic differentiation between the 13 Czech beech populations, because they were greater than zero, that means they confirmed the presence of population structuring in Czech European beech. Not significant linear correlations were observed between genetic and geographic distances of the 13 beech populations studied on the basis of microsatellite markers. Twelve microsatellite markers were verified as highly polymorphic and suitable for genotyping of European beech populations.
References:
Asuka Y., Tani N., Tsumura Y., Tomaru N. (2004): Development and characterization of microsatellite markers for Fagus crenata Blume. Molecular Ecology Notes, 4, 101-103 https://doi.org/10.1046/j.1471-8286.2003.00583.x
 
Buiteveld J., Vendramin G.G., Leonardi S., Kamer K., Geburek T. (2007): Genetic diversity and differentiation in European beech (Fagus sylvatica L.) stands varying in management history. Forest Ecology and Management, 247, 98-106 https://doi.org/10.1016/j.foreco.2007.04.018
 
Čáp J., Novotný P., Cvrčková H., Máchová P., Fulín M., Frýdl J., Dostál J., Buriánek V., Beran F., Lefnar R., Poláková L., Malá J. (2015): Genetická charakterizace významných regionálních populací smrku ztepilého v České republice. Specializovaná mapa s odborným obsahem. Jíloviště-Strnady, VÚLHM: 32.
 
Chybicki Igor J., Trojankiewicz Magdalena, Oleksa Andrzej, Dzialuk Artur, Burczyk Jaroslaw (2009): Isolation-by-distance within naturally established populations of European beech ( Fagus sylvatica ). Botany, 87, 791-798 https://doi.org/10.1139/B09-049
 
Comps B., Gömöry D., Letouzy J., Thiébaut B., Petit R.J. (2001): Diverging trends between heterozygosity and allelic richness during postglacial colonization in the European beech. Genetics, 157: 389–397.
 
Dounavi A., Koutsias N., Ziehe M., Hattemer H. H. (2010): Spatial patterns and genetic structures within beech populations (Fagus sylvatica L.) of forked and non-forked individuals. European Journal of Forest Research, 129, 1191-1202 https://doi.org/10.1007/s10342-010-0409-9
 
Fulín M., Čáp J., Cvrčková H., Novotný P., Máchová P., Dostál J., Frýdl J., Beran F. (2016): Genetická charakterizace významných regionálních populací jedle bělokoré v České republice. Specializovaná mapa s odborným obsahem. Jíloviště-Strnady, VÚLHM: 42.
 
Gömöry Dušan, Hynek Vladimír, Paule Ladislav (1998): Delineation of seed zones for European beech (Fagus sylvatica L.) in the Czech Republic based on isozyme gene markers. Annales des Sciences Forestières, 55, 425-436 https://doi.org/10.1051/forest:19980403
 
Hasenkamp N., Ziegenhagen B., Mengel C., Schulze L., Schmitt H.-P., Liepelt S. (2011): Towards a DNA marker assisted seed source identification: a pilot study in European beech (Fagus sylvatica L.). European Journal of Forest Research, 130, 513-519 https://doi.org/10.1007/s10342-010-0439-3
 
Hintze J.L. (2012): NCSS 8. Kaysville, NCSS: 2823.
 
JUMP ALISTAIR S., PEÑUELAS JOSEP (2007): Extensive spatial genetic structure revealed by AFLP but not SSR molecular markers in the wind-pollinated tree, Fagus sylvatica. Molecular Ecology, 16, 925-936 https://doi.org/10.1111/j.1365-294X.2006.03203.x
 
KALINOWSKI STEVEN T., TAPER MARK L., MARSHALL TRISTAN C. (2007): Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16, 1099-1106 https://doi.org/10.1111/j.1365-294X.2007.03089.x
 
Konnert M., Ziehe M., Trober U., Mauer W., Janßen A., Sander T., Hussendörfer E., Hertel H. (2000): Genetische Variation der Buch (Fagus sylvatica L.) in Deutschland: Gemeinsame Auswertung genetischer Inventuren über verschiedene Bundesländer. Forts und Holz, 55: 403–408.
 
LEFÈVRE S., WAGNER S., PETIT R. J., De LAFONTAINE G. (2012): Multiplexed microsatellite markers for genetic studies of beech. Molecular Ecology Resources, 12, 484-491 https://doi.org/10.1111/j.1755-0998.2011.03094.x
 
Meloun M., Militký J. (2011): Statistical Data Analysis, A Practical Guide with 1250 Exercises and Answer Key on CD. New Delhi, Woodhead Publishing India: 1600.
 
Ministry of Agriculture of the Czech Republic (2015): Information on forests and forestry in the Czech Republic by 2014. Available at http://eagri.cz/public/web/file/433136/ZZ2014AJ_16112015.pdf (accessed June 27, 2016).
 
Nei Masatoshi (1972): Genetic Distance between Populations. The American Naturalist, 106, 283- https://doi.org/10.1086/282771
 
ODDOU-MURATORIO SYLVIE, KLEIN ETIENNE K., VENDRAMIN GIOVANNI G., FADY BRUNO (2011): Spatial vs. temporal effects on demographic and genetic structures: the roles of dispersal, masting and differential mortality on patterns of recruitment in Fagus sylvatica. Molecular Ecology, 20, 1997-2010 https://doi.org/10.1111/j.1365-294X.2011.05039.x
 
Pastorelli R., Smulders M. J. M., VAN���T Westende W. P. C., Vosman B., Giannini R., Vettori C., Vendramin G. G. (2003): Characterization of microsatellite markers in Fagus sylvatica L. and Fagus orientalis Lipsky. Molecular Ecology Notes, 3, 76-78 https://doi.org/10.1046/j.1471-8286.2003.00355.x
 
Peakall R., Smouse P. E. (): GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics, 28, 2537-2539 https://doi.org/10.1093/bioinformatics/bts460
 
Piotti A, Leonardi S, Buiteveld J, Geburek T, Gerber S, Kramer K, Vettori C, Vendramin G G (): Comparison of pollen gene flow among four European beech (Fagus sylvatica L.) populations characterized by different management regimes. Heredity, 108, 322-331 https://doi.org/10.1038/hdy.2011.77
 
Pluess Andrea R., Määttänen Kirsti (2013): Characterization of eighteen novel microsatellite markers and multiplex PCR protocol for Fagus sylvatica. Conservation Genetics Resources, 5, 311-314 https://doi.org/10.1007/s12686-012-9791-6
 
Scalfi M., Troggio M., Piovani P., Leonardi S., Magnaschi G., Vendramin G. G., Menozzi P. (2004): A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech ( Fagus sylvatica L.). TAG Theoretical and Applied Genetics, 108, 433-441 https://doi.org/10.1007/s00122-003-1461-3
 
Seifert S., Vornam B., Finkeldey R. (2012): DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.). European Journal of Forest Research, 131, 1761-1770 https://doi.org/10.1007/s10342-012-0630-9
 
Svoboda P. (1955): Lesní dřeviny a jejich porosty. Část II. Prague, Státní zemědělské nakladatelství v Praze: 573.
 
Tanaka K., Tsumura Y., Nakamura T. (1999): Development and polymorphism of microsatellite markers for Fagus crenata and the closely related species, F. japonica. TAG Theoretical and Applied Genetics, 99, 11-15 https://doi.org/10.1007/s001220051203
 
Úradníček L., Maděra P., Tichá S., Koblížek J. (2009): Dřeviny České republiky. Kostelec nad Černými lesy, Lesnická práce, s.r.o.: 366.
 
VAN OOSTERHOUT COCK, HUTCHINSON WILLIAM F., WILLS DEREK P. M., SHIPLEY PETER (2004): micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4, 535-538 https://doi.org/10.1111/j.1471-8286.2004.00684.x
 
Vendramin G. G., Anzidei M., Vettori C., Pastorelli R., Paffetti D., Giannini R. (2004): Geographic distribution of chloroplast variation in Italian populations of beech ( Fagus sylvatica L.). TAG Theoretical and Applied Genetics, 109, 1-9 https://doi.org/10.1007/s00122-004-1609-9
 
Vornam Barbara, Decarli Natalia, Gailing Oliver (2004): Spatial Distribution of Genetic Variation in a Natural Beech Stand (Fagus sylvaticaL.) Based on Microsatellite Markers. Conservation Genetics, 5, 561-570 https://doi.org/10.1023/B:COGE.0000041025.82917.ac
 
Wright Sewall (1965): The Interpretation of Population Structure by F-Statistics with Special Regard to Systems of Mating. Evolution, 19, 395- https://doi.org/10.2307/2406450
 
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