Laboratory assay of entomopathogenic nematodes against the elm leaf beetle, Xanthogaleruca luteola Müller (Col.: Chrysomelidae)

Zeinolabedin Fard N., Abbasipour H., Saeedizadeh A., Karimi J. (2020): Laboratory assay of entomopathogenic nematodes against the elm leaf beetle, Xanthogaleruca luteola Müller (Col.: Chrysomelidae). J. For. Sci., 66: 524–531.

download PDF

The susceptibility of the elm leaf beetle, Xanthogaleruca luteola Müller (Col.: Chrysomelidae), 3rd instar larvae and adults to the entomopathogenic nematodes Steinernema feltiae, S. carpocapsae, and Heterorhabditis bacteriophora was determined in laboratory assays. Larval mortality was assessed at 0, 16, 32, 48, 64, 80, 96, and 112 h after exposure of the larvae and adults to six concentrations (0, 100, 200, 300, 400, and 500) of infective juveniles (IJs) per mL. The median lethal concentration (LC50) values for each nematode species against the larvae and adults of X. luteola were 167.59 and 6.73 IJ·mL–1 for S. feltiae, 218.23 and 204.09 IJ·mL–1 for S. carpocapsae, and 338.66 and 70.29 IJ·mL–1 for H. bacteriophora, respectively. Also, the median lethal time (LT50) values for each nematode species against the larvae and adults of X. luteola were 44.51 and 22.23 h for S. feltiae, 50.78 and 36.17 h for S. carpocapsae, and 67.64 and 34.71 h for H. bacteriophora, respectively. The S. feltiae nematode was the most effective species in controlling the larvae and adults of the elm leaf beetle, X. luteola. Based on these and other results, the research could be expanded on the prospects of using entomopathogenic nematodes, especially S. feltiae, in managing of the elm leaf beetle, X. luteola.

Azarnia S., Abbasipour H., Saeedizadeh A., Askarianzadeh A. (2018): Laboratory assay of entomopathogenic nematodes against clearwing moth (Lepidoptera: Sesiidae) Larvae. Journal of Entomological Science, 53: 62–69.
Bayramoglu Z., Demir I., Inan C., Demirbag Z. (2018): Efficacy of native entomopathogenic nematodes from Turkey against the alder leaf beetle, Agelastica alni L. (Coleoptera: Chrysomelidae), under laboratory conditions. Egyptian Journal of Biological Pest Control, 28: 17.
Bélair G., Fourniery D., Auphinais N. (2003): Efficacy of steinernematid nematodes against three insect pests of crucifers in Quebec. Journal of Nematology, 35: 259–265.
Chiffelle I., Huerta A., Celis M., Araya J. (2013): Proximal analysis and insecticidal effects of extracts from pepper tree (Schinus molle) leaves on elm leaf beetle (Xanthogaleruca luteola) larvae. Industrial Crop Products, 43: 523−528.
Ebrahimi L., Niknam G.H., Lewis E.E. (2011): Lethal and sublethal effects of Iranian isolates of Steinernema feltiae and Heterorhabditis bacteriophora on the Colorado potato beetle, Leptinotarsa decemlineata. BioControl, 56: 781–788.
Huerta A., Chiffelle I., Puga K., Azúa F., Araya J. (2011): Life cycle of Xanthogaleruca luteola Mull (Col.: Chrysomelidae) in Santiago, Chile, and sex phenotype differentiation of adults. Boletín de sanidad Vegetal Plagas, 37: 57−64.
Kaya H.K., Hara A.H., Reardon R.C. (1981): Laboratory and field evaluation of Neoaplectana carpocapsae (Rhabditida: Steinernematidae) against the elm leaf beetle (Coleoptera: Chrysomelidae) and the western spruce budworm (Lepidoptera: Tortricidae). Canadian Entomologist, 113: 787−793.
Kaya H.K., Stock S.P. (1997): Techniques in insect nematology. In: Lacey L.A. (ed.): Manual of Techniques in Insect Pathology. San Diego, Academic Press : 281–324.
Morris E.E., Grewal P.S. (2011): Susceptibility of the Adult Japanese Beetle, Popillia japonica to Entomopathogenic Nematodes. Journal of Nematology, 43: 196–200.
Shapiro-Ilan D.I., Cottrell T.E. (2006): Susceptibility of the lesser peach tree borer (Lepidoptera: Sesiidae) to entomopathogenic nematodes under laboratory conditions. Environmental Entomology, 35: 358–365.
Sharghi H., Eivazian Kary N., Mohammadi D. (2019): Susceptibility of Polyphylla olivieri and Xanthogaleruca luteola to an entomopathogenic nematode, Heterorhabditis bacteriophora. In: Proceedings of 3rd Iranian International Congress of Entomology, University of Tabriz, Tabriz, Iran, Aug 17–19, 2019: 180.
Shekari M., Jalali Sendi J., Etebari K., Zibaee A., Shadparvar A. (2008): Effects of Artemisia annua L. (Asteracea) on nutri-tional physiology and enzyme activities of elm leaf beetle, Xanthogaleruca luteola Mull. (Coleoptera: Chrysomellidae). Pesticide Biochemistry and Physiology, 91: 66–74.
Sheykhnejad H., Ghadamyari M., Koppenhöfer A.M., Karimi J. (2014): Interactions between entomopathogenic nematodes and imidacloprid for rose sawfly control. Biocontrol Science and Technology, 24: 1481–1486.
SPSS (2006): SPSS base 15.0 user’s guide. SPSS, Chicago, IL.
Thurston G.S. (1998): Biological control of elm leaf beetle. Journal of Arboriculture, 24: 154–159.
Tomalak M. (2004): Infectivity of entomopathogenic nematodes to soil-dwelling developmental stages of the tree leaf beetles, Altica quercetorum and Agelastica alni. Entomologia Experimentalis et Applicata, 110: 125–133.
Trdan S., Vidrih M., Andjus L., Laznik Z. (2009): Activity of four entomopathogenic nematode species against different developmental of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae). Helminthologia, 46: 14–20.
Trdan S., Vidrih M., Valic N., Laznik Z. (2008): Impact of entomopathogenic nematodes on adults of Phyllotreta spp. (Coleoptera: Chrysomelidae) under laboratory conditions. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 58: 169–175.
Triggiani O., Tarasco E. (2007): Applying entomopathogenic nematode to Xanthogaleruca luteola (Coleoptera: Chrysomelidae) infested foliage. Redia XC: 29–31.
Zolfagharian M., Saeedizadeh A., Abbasipour H. (2016): Efficacy of two entomopathogenic nematode species as potential biocontrol agents against the diamondback moth, Plutella xylostella (L.). Journal of Biological Control, 30: 78–83.
Zolfagharian M., Saeedizadeh A., Abbasipour H., Joyandeh A., Ahmadian Yazdi A. (2015): Efficacy of entomopathogenic nematode, Steinernema carpocapsae against the diamondback moth, Plutella xylostella (L.) in laboratory condition. Archives of Phytopathology and Plant Protection, 48: 393–399.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti