Determination of effects of desiccation and frost stresses on the physiological quality of Norway spruce (Picea abies [L.] Karst.) seedlings by measurement of electrolyte leakage from the root system

https://doi.org/10.17221/11928-JFSCitation:Sarvaš M. (2002): Determination of effects of desiccation and frost stresses on the physiological quality of Norway spruce (Picea abies [L.] Karst.) seedlings by measurement of electrolyte leakage from the root system. J. For. Sci., 48: 366-371.
download PDF

Two-year, bare-rooted seedlings of Norway spruce (Picea abies [L.] Karst.) were used in this study. The seedlings were exposed to artificial desiccation and frost stress after spring lifting. After exposure to these factors, electrolyte leakage from the root system was measured. The results showed that artificial desiccation significantly affected the rate of electrolyte leakage. The effect of desiccation was confirmed by the survival of plants and height increment (100% survival and 49% height increment of control seedlings in contrast with plants after 6 hours of desiccation: 9% survival and 2% height increment). On the other hand, frost stress did not affect the rate of electrolyte leakage (the rate of REL did not correlate with the intensity of stress treatment).

References:
Anonymous, 1997. Správa o lesnom hospodárstve v Slovenskej republike (Green Report).
 
Binder Wolfgang D., Fielder Peter (1995): Heat damage in boxed white spruce (Picea glauca [Moench.] Voss) seedlings: Its pre-planting detection and effect on field performance. New Forests, 9, 237-259  https://doi.org/10.1007/BF00035490
 
Burdett A.N., Simpson D.G., Thompson, C.F., 1983. Root development and plantation establishment success. Pl. and Soil, 71: 103–110.
 
Calme S., Bigras F. J., Margolis H. A., Hebert C. (1994): Frost tolerance and bud dormancy of container-grown yellow birch, red oak and sugar maple seedlings. Tree Physiology, 14, 1313-1325  https://doi.org/10.1093/treephys/14.12.1313
 
Colombo Stephen J. (2008): Timing of cold temperature exposure affects root and shoot frost hardiness of Picea Mariana container seedlings. Scandinavian Journal of Forest Research, 9, 52-59  https://doi.org/10.1080/02827589409382812
 
Colombo S.J., 1997. Frost hardening spruce container stock for overwintering in Ontario. New Forests, 13: 449–467. https://doi.org/10.1023/A:1006571300025
 
Garriou Didier, Girard Sabine, Guehl Jean-Marc, G�n�r� Beno�t (2000): Effect of desiccation during cold storage on planting stock quality and field performance in forest species. Annals of Forest Science, 57, 101-111  https://doi.org/10.1051/forest:2000162
 
Folk R.S., Grossnickle C.S., 1997. Stock quality assessment: Still an important component of operational reforestation programs. In: National Proc.: Forest and Conservations Nursery Associations, Bemidji, 11–14 August, 1997: 120–124.
 
Keates S.E., 1990. Assessing cold hardiness in conifers:
 
a problem analysis and discussion paper. Forestry Canada, B.C. Minist. For. FRDA Rep. No. 106.
 
Kerr G., Harper Ch., 1994. Assessing the quality of broadleaved nursery stock. Arboriculture Research and Information Note 126/94.
 
Lassheikki M., Puttonen P., Räsänen P. K. (2008): Planting performance potential of Pinus sylvestris seedlings as evaluated by root growth capacity and triphenyl tetrazolium chloride reduction methods. Scandinavian Journal of Forest Research, 6, 91-104  https://doi.org/10.1080/02827589109382652
 
Lindqvist H., 1998. Effect of lifting date and time of storage on survival and die-back in four deciduous species. J. Environ. Hort., 16: 195–201.
 
LindstrÖm A., 1986. Freezing temperatures in the root zone-
 
effects on growth of containerized Pinus sylvestris and Picea abies seedlings. Scand. J. For. Res., 6: 91–104.
 
Martincová J., NÁROVCOVÁ J., 2001. Metody hodnocení kvality sadebního materiálu. In: Jurásek A., Novák J., Slodičák M. (eds.), 50 let pěstebního výzkumu v Opočně, 12–13 září 2001: 205–208.
 
McEvoy C., McKay H. (2012): ROOT FROST HARDINESS OF AMENITY BROADLEAVED SEEDLINGS. Arboricultural Journal, 21, 231-244  https://doi.org/10.1080/03071375.1997.9747169
 
McEvoy C., McKAY H.M., 1997b. Sensitivity of broadleaved trees to desiccation and rough handling between lifting and transplanting. Arboriculture Research and Information Note 139/97.
 
McKay H.M., 1991. Electrolyte Leakage: A Rapid Index of Plant Vitality. For Comm. Res. Div., Res. Inf. Note 210.
 
McKay H.M. (1992): Electrolyte leakage from fine roots of conifer seedlings: a rapid index of plant vitality following cold storage. Canadian Journal of Forest Research, 22, 1371-1377  https://doi.org/10.1139/x92-182
 
McKay H.M. (1993): Tolerance of conifer fine roots to cold storage. Canadian Journal of Forest Research, 23, 337-342  https://doi.org/10.1139/x93-049
 
McKay H. M. (2008): Frost hardiness and cold‐storage tolerance of the root system of Picea sitchensis, Pseudotsuga menziesii, Larix kaempferi and Pinus sylvestris bare‐root seedlings. Scandinavian Journal of Forest Research, 9, 203-213  https://doi.org/10.1080/02827589409382832
 
McKay H.M., 1997. A review of the effect of stresses between lifting and planting on nursery stock quality and performance. New Forests, 13: 369–399. https://doi.org/10.1023/A:1006563130976
 
McKay H. (1998): Root electrolyte leakage and root growth potential as indicators of spruce and larch establishment. Silva Fennica, 32, -  https://doi.org/10.14214/sf.684
 
McKay H. M., Mason W. L. (1991): Physiological indicators of tolerance to cold storage in Sitka spruce and Douglas-fir seedlings. Canadian Journal of Forest Research, 21, 890-901  https://doi.org/10.1139/x91-124
 
McKay H.M., White M.S., 1997. Fine root electrolyte leakage and moisture content: indices of Sitka spruce and Douglas-fir seedlings performance after desiccation. New Forests, 13: 139–162. https://doi.org/10.1023/A:1006571805960
 
MURRAY M. B., CAPE J. N., FOWLER D. (1989): Quantification of frost damage in plant tissues by rates of electrolyte leakage. New Phytologist, 113, 307-311  https://doi.org/10.1111/j.1469-8137.1989.tb02408.x
 
Nicoll Bruce C., Redfern Derek B., McKay Helen M. (1996): Autumn frost damage: clonal variation in Sitka spruce. Forest Ecology and Management, 80, 107-112  https://doi.org/10.1016/0378-1127(95)03635-0
 
Ritchie G.A., Dunlap J.R., 1980. Root growth potential: its development and expression in forest tree seedlings. N.Z. J. For. Sci., 10: 218–248.
 
Sarvaš M., 1998. Vplyv mrazu a sucha na stratu elektrolytu
 
z hlavného koreňa a následnú ujatosť duba. In: Zbor. ref.
 
z medzinár. ved. konf. Lesy a lesnícky výskum pre tretie tisícročie, Zvolen, 11–14 Október: 125–130.
 
Sarvaš M., 1999. Možnosti použitia merania straty elektrolytu na zistenie kvality sadbového materiálu. J. For. Sci., 45: 131–138.
 
Schüte G., Sarvaš m., 1999. Determination of regrowth potential of planting stock using REL-technique for oak (Quercus robur L.) seedlings. Forstarchiv, 70: 133–138.
 
Simpson D.G., Ritchie G.A., 1997. Does RGP predict field performance? A Debate. New Forests, 13: 253–277. https://doi.org/10.1023/A:1006542526433
 
Smit-Spinks B., Swanson B. T., Markhart III A. H. (1985): The effect of photoperiod and thermoperiod on cold acclimation and growth of Pinussylvestris. Canadian Journal of Forest Research, 15, 453-460  https://doi.org/10.1139/x85-072
 
TABBUSH PAUL M. (1987): Effect of Desiccation on Water Status and Forest Performance of Bare-rooted Sitka Spruce and Douglas Fir Transplants. Forestry, 60, 31-43  https://doi.org/10.1093/forestry/60.1.31
 
Tinus R.W., 1996. Cold hardiness testing to time lifting and packing of container stock: A case history. Tree Planters’ Notes, 47: 62–67.
 
download PDF

© 2020 Czech Academy of Agricultural Sciences