Long-term fertilization effects were evaluated including NPK, Ca and N applications at the lower altitudes. The studied localities are in the territory of Training Forest Enterprise in Kostelec nad Černými lesy, at an altitude of 300–500 m a.s.l., sites of beech-oak-fir types. Fertilizers were used in 1965–1967, the evaluation of soil condition was done in 1967 (before fertilization) and 2002. After 25–35 years, only low effects of fertilization are detectable in the humus forms and complex soil profile – surface humus accumulation, soil chemistry, as well as nutrient contents. Complex fertilization was reflected in higher site fertility in general, N-fertilization only in the progress of acidification. During the period 1967–2002, a strong general trend of acidification is obvious caused by both acid deposition and Norway spruce monoculture-based forestry.