Control of Acanthoscelides obtectus with Trichoderma harzianum applied alone or in combination with diatomaceous earth on a stored common bean

https://doi.org/10.17221/104/2019-PPSCitation:Abdelrahim Gad H., Said Mohamed Al-Anany M., mohamed Sameer W., Said Mohamed Al-Anany F. (2020): Control of Acanthoscelides obtectus with Trichoderma harzianum applied alone or in combination with diatomaceous earth on a stored common bean. Plant Protect. Sci., 56: 107-115.
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A laboratory assay was designed to determine the insecticidal efficacy of Trichoderma harzianum Rifai and diatomaceous earth (DE) against Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae). The fungus T. harzianum was applied at 0.0, 3.3 × 106, 6.6 × 106 and 2.1 × 107 spores/kg of the common bean individually and mixed with 0, 200, 400 and 800 ppm of DE. The mortality counts were made after 1, 2, 4 and 7 days. All of the dead adults were removed after each count, and the vials were kept for the next 60 days to assess the emergence of any progeny. The highest mortality (93.88%) was achieved after 7 days using 800 ppm of DE and 2.1 × 107 spores/kg of T. harzianum and it suppressed emergence of the second generation after 60 days of treatment. These results concluded that DE can be used successfully along with T. harzianum against A. obtectus in stored common beans.

References:
Abbott W. (1925): A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265–267. https://doi.org/10.1093/jee/18.2.265a
 
Akbar W., Lord J.C., Nechols J.R., Howard R.W. (2004): Diatomaceous earth increases the efficacy of Beauveria bassiana against Tribolium castaneum larvae and increases conidia attachment. Journal of Economic Entomology, 97: 273–280. https://doi.org/10.1603/0022-0493-97.2.273
 
Akbar W., Lord J.C., Nechols J.R., Loughin T.M. (2005): Efficacy of Beauveria bassiana for red flour beetle when applied with plant essential oils or mineral oil and organosilicone carriers. Journal of Economic Entomology, 98: 683–688. https://doi.org/10.1603/0022-0493-98.3.683
 
Ashraf M.A., Momein H.E. (2007): Entomopathogenic fungi as biopesticides against the Egyptian cotton leafworm, Spodoptera littoralis between biocontrol promise and immune–limitation. Journal of the Egyptian Society of Toxicology, 37: 39–51.
 
Athanassiou C.G., Steenberg T. (2007): Insecticidal effect of Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreaes) in combination with three diatomaceous earth formulations against Sitophilus granarius (L.) (Coleoptera: Curculionidae). Biological Control, 40: 411–416. https://doi.org/10.1016/j.biocontrol.2006.12.001
 
Athanassiou C.G., Kavallieratos N.G., Vayias B.J., Tsakiri J.B., Mikeli N.H., Meletsis C.M., Tomanovic Z. (2008): Persistence and efficacy of Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes) and diatomaceous earth against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) on wheat and maize. Crop Protection, 27: 1303–1311. https://doi.org/10.1016/j.cropro.2008.03.004
 
Batta Y.A. (2004): Control of rice weevil (Sitophilus oryzae L. Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. Crop Protection, 23: 103–108. https://doi.org/10.1016/j.cropro.2003.07.001
 
Daglish G.J. (2008): Impact of resistance on the efficacy of binary combinations of spinosad, chlorpyrifos-methyl and s-methoprene against five stored-grain beetles. Journal of Stored Products Research, 44: 71–76. https://doi.org/10.1016/j.jspr.2007.06.003
 
Daglish G.J., Hall E.A., Zorzetto M.J., Lambkin T.M., Erbacher J.M. (1993): Evaluation of protectants for control of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae) in navy beans (Phaseolus vulgaris (L.). Journal of Stored Products Research, 29: 215–219. https://doi.org/10.1016/0022-474X(93)90003-M
 
Dal Bello G., Fusé C.B., Pedrini N., Padín S.B. (2017): Insecticidal efficacy of Beauveria bassiana, diatomaceous earth and fenitrothion against Rhyzopertha dominica and Tribolium castaneum on stored wheat. International Journal of Pest Management, 64: 279–286. https://doi.org/10.1080/09670874.2017.1397300
 
Dal Bello G., Padin S., Juarez P., Pedrini N., De Giusto M. (2006): Biocontrol of Acanthoscelides obtectus and Sitophilus oryzae with diatomaceous earth and Beauveria bassiana on stored grains. Biocontrol Science and Technology, 16: 215–220. https://doi.org/10.1080/09583150500336010
 
Dusan L., Korunic Z. (2018): Diatom Nanotechnology: Progress and Emerging Applications. Nanoscience & Nanotechnology Series. 1st Ed. London, Royal Society of Chemistry: 219–247.
 
Ebeling W. (1971): Sorptive dusts for pest control. Annual Review of Entomology, 16: 123–158. https://doi.org/10.1146/annurev.en.16.010171.001011
 
Fields P., Korunic Z. (2000): The effect of grain moisture content and temperature on the efficacy of diatomaceous earths from different geographical locations against stored-product beetles. Journal of Economic Entomology, 36: 1–13.
 
Fiorentino N., Ventorino V., Woo S.L., Pepe O., De Rosa A., Gioia L., Rouphael Y. (2018): Trichoderma-based biostimulants modulate rhizosphere microbial populations and improve N uptake efficiency, yield, and nutritional quality of leafy vegetables. Frontiers in Plant Science, 9: 743–757. https://doi.org/10.3389/fpls.2018.00743
 
Gad H.A. (2019): First report on the susceptibility of certain dry Egyptian common bean (Phaseolus vulgaris L.) (Fabaceae) varieties to infestation by Acanthoscelides obtectus (SAY, 1831) (Coleoptera: Chrysomelidae: Bruchinae). Polish Journal of Entomology, 88: 149–161. https://doi.org/10.2478/pjen-2019-0012
 
Gad A.H., Abied M. K. (2019): Effect of some legumes on the biological parameters of the Acanthoscelides obtectus Say. Egyptian Academic Journal of Biological Sciences, 12: 85–93. https://doi.org/10.21608/eajbsa.2019.36231
 
Golob P. (1997): Current status and future perspective for inert dusts for control of stored product insects. Journal of Stored Products Research, 33: 69–79. https://doi.org/10.1016/S0022-474X(96)00031-8
 
Kavallieratos N., Athanassiou C., Michalaki M., Batta Y., Rigatos H., Pashalidou F., Balotis G., Tomanovic Z., Vayias B. (2006): Effect of the combined use of Metarhizium anisopliae (Metschinkoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species. Crop Protection, 25: 1087–1094. https://doi.org/10.1016/j.cropro.2006.02.009
 
Korunic Z. (1998): Diatomaceous earths, a group of natural insecticides. Journal of Stored Products Research, 34: 87–97. https://doi.org/10.1016/S0022-474X(97)00039-8
 
Korunic Z., Fields P.G., Kovacs M.I.P., Noll J.S., Lukow O.M., Demianyk C.J., Shibley K.J. (1996): The effect of diatomaceous earth on grain quality. Postharvest Biology and Technology, 9: 373–387. https://doi.org/10.1016/S0925-5214(96)00038-5
 
Ling Z., Zhanggui Q., Korunic Z. (2000): Field and laboratory experiments with Protect-It, an enhanced diatomaceous earth in People’s Republic of China. In: Zuxun J., Quan L., Yongsheng L., Xianchang T., Lianghua G. (eds): Proceedings of the Seventh International Working Conference for Stored-Product Protection, Chengdu, Oct 14–19, 1999: 745–757
 
Regnault-Roger C., Vincent C., Arnason J.T. (2012): Essential oils in insect control: low-risk products in a high-stakes world. Annual Review of Entomology, 57: 405–424. https://doi.org/10.1146/annurev-ento-120710-100554
 
Riasat T., Wakil W., Ashfaq M., Sahi S. (2011): Effect of Beauveria bassiana mixed with diatomaceous earth on mortality, mycosis and sporulation of Rhyzopertha dominica on stored wheat. Phytoparasitica, 39: 325–331. https://doi.org/10.1007/s12600-011-0164-6
 
Rizwan M., Atta B., Rizwan M., Sabir A.M., Shah Z.U., Hussain M. (2019): Effect of the entomopathogenic fungus, Beauveria bassiana, combined with diatomaceous earth on the red flour beetle, Tribolium castaneum (Herbst) (Tenebrionidae: Coleoptera). Egyptian Journal of Biological Pest Control, 29: 27–32. https://doi.org/10.1186/s41938-019-0131-y
 
Rodríguez-González Á., Mayo S., González-López Ó., Reinoso B., Gutierrez S., Casquero P.A. (2017): Inhibitory activity of Beauveria bassiana and Trichoderma spp. on the insect pests Xylotrechus arvicola (Coleoptera: Cerambycidae) and Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae). Environmental Monitoring and Assessment, 189: 12–20. https://doi.org/10.1007/s10661-016-5719-z
 
Rodríguez-González Á., Casquero P.A., Suárez-Villanueva V., Carro-Huerga G., Álvarez-García S., Mayo-Prieto S., Lorenzana A., Cardoza R.E., Gutiérrez S. (2018): Effect of trichodiene production by Trichoderma harzianum on Acanthoscelides obtectus. Journal of Stored Products Research, 77: 231–239. https://doi.org/10.1016/j.jspr.2018.05.001
 
Rodríguez-González A., Casquero P.A., Cardoza R.E., Gutiérrez S. (2019): Effect of trichodiene synthase encoding gene expression in Trichoderma strains on their effectiveness in the control of Acanthoscelides obtectus. Journal of Stored Products Research, 83: 275–280. https://doi.org/10.1016/j.jspr.2019.07.006
 
Thakur D.R. (2012): Taxonomy, distribution and pest status of Indian biotypes of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae): A new record. Pakistan Journal of Zoology, 44: 189–195.
 
Vassilakos T.N., Athanassiou C.G., Kavallieratos N.G., Vayias B.J. (2006): Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat. Biological Control, 38: 270–281. https://doi.org/10.1016/j.biocontrol.2006.03.009
 
Vilca Mallqui K.S., Oliveira E.E., Guedes R.N.C. (2013): Competition between the bean weevils Acanthoscelides obtectus and Zabrotes subfasciatus in common beans. Journal of Stored Products Research, 55: 32–35. https://doi.org/10.1016/j.jspr.2013.07.004
 
Wakil W., Ashfaq M., Ghazanfar M. U., Riasat T. (2010): Susceptibility of stored product insects to enhanced diatomaceous earth. Journal of Stored Products Research, 46: 248–249. https://doi.org/10.1016/j.jspr.2010.05.001
 
Wakil W., Ghazanfar M. U. (2010): Entomopathogenic fungus as a biological control agent against Rhyzopertha dominica F. (Coleoptera: Bostrychidae) on stored wheat. Archives of Phytopathology and Plant Protection, 43: 1236–1242. https://doi.org/10.1080/03235400802404718
 
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