Responses of rice to Rhizoctonia solani and its toxic metabolite in relation to expression of Osmyb4 transcription factor
Singh Pooja, Subramanian Babuhttps://doi.org/10.17221/107/2015-PPSCitation:Pooja S., Babu S. (2017): Responses of rice to Rhizoctonia solani and its toxic metabolite in relation to expression of Osmyb4 transcription factor. Plant Protect. Sci., 53: 208-215.
The reaction of IR 50, TRY 3, and IR 36 cultivars of rice to R. solani challenge, the causal agent of sheath blight, and its toxic metabolite was studied. Differential response of these cultivars to the pathogen and/or toxin inoculation was observed in detached leaf sheaths and greenhouse-grown plants. The observations were based on disease scoring, electrolyte leakage, and also microscopic views of infection cushions. The Osmyb4 gene expression was studied in the tissues from all these experiments and a correlation between the level of expression and disease response of the varieties was found at least in some experiments. The mechanisms regulated by Osmyb4 might have a lower but significant contribution to the tolerance of rice cultivars to sheath blight.Keywords:
disease; Oryza sativa; resistance; Rhizoctonia; sheath blight; transcription factorReferences:
Baldoni E., Genga A., Medici A., Coraggio I., Locatelli F. (2013): The OsMyb4 gene family: stress response and transcriptional auto-regulation mechanisms. Biologia Plantarum, 57, 691-700 https://doi.org/10.1007/s10535-013-0331-3Bernardes-de-Assis Joana, Storari Michelangelo, Zala Marcello, Wang Wenxiang, Jiang Daohong, ShiDong Li, Jin Meisong, McDonald Bruce A., Ceresini Paulo C. (2009): Genetic Structure of Populations of the Rice-Infecting Pathogen Rhizoctonia solani AG-1 IA from China. Phytopathology, 99, 1090-1099 https://doi.org/10.1094/PHYTO-99-9-1090Choppakatla V., Melouk H.A., Hunger R.M. (2006): Infection cushion formation by Rhizoctonia spp. on peanut and wheat root systems. Phytopathology, 96: S24.Docimo T., Mattana M., Fasano R., Consonni R., de Tommasi N., Coraggio I., Leone A. (2013): Ectopic expression of the Osmyb4 rice gene enhances synthesis of hydroxycinnamic acid derivatives in tobacco and clary sage. Biologia Plantarum, 57, 179-183 https://doi.org/10.1007/s10535-012-0257-1Groth D.E. (2008): Effects of cultivar resistance and single fungicide application on rice sheath blight, yield, and quality. Crop Protection, 27, 1125-1130 https://doi.org/10.1016/j.cropro.2008.01.010Khush G.S., Virk P.S. (2005): IR Varieties and their Impact. Los Banos (Philippines), International Rice Research Institute. Available at http://books.irri.org/9712202062_content.pdfLaura Marina, Consonni Roberto, Locatelli Franca, Fumagalli Elisabetta, Allavena Andrea, Coraggio Immacolata, Mattana Monica (2010): Metabolic response to cold and freezing of Osteospermum ecklonis overexpressing Osmyb4. Plant Physiology and Biochemistry, 48, 764-771 https://doi.org/10.1016/j.plaphy.2010.06.003Mattana Monica, Biazzi Elisa, Consonni Roberto, Locatelli Franca, Vannini Candida, Provera Stefano, Coraggio Immacolata (2005): Overexpression of Osmyb4 enhances compatible solute accumulation and increases stress tolerance of Arabidopsis thaliana. Physiologia Plantarum, 125, 212-223 https://doi.org/10.1111/j.1399-3054.2005.00551.xNandakumar R., Babu S., Radjacommare R., Raguchander T., Samiyappan R. (2002): Pseudomonas fluorescens mediated antifungal activity against R. solani, sheath blight pathogen. Phytopathologia Mediterrania, 41: 109–119.PARK MYOUNG-RYOUL, YUN KIL-YOUNG, MOHANTY BIJAYALAXMI, HERATH VENURA, XU FUYU, WIJAYA EDWARD, BAJIC VLADIMIR B., YUN SONG-JOONG, DE LOS REYES BENILDO G. (2010): Supra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle development. Plant, Cell & Environment, 33, 2209-2230 https://doi.org/10.1111/j.1365-3040.2010.02221.xPasquali Gemma, Biricolti Stefano, Locatelli Franca, Baldoni Elena, Mattana Monica (2008): Osmyb4 expression improves adaptive responses to drought and cold stress in transgenic apples. Plant Cell Reports, 27, 1677-1686 https://doi.org/10.1007/s00299-008-0587-9Pooja Singh, Sweta Kumari, Mohanapriya A., Sudandiradoss C., Siva Ramamoorthy, Gothandam Kodiveri M., Babu Subramanian (2015): Homotypic clustering of OsMYB4 binding site motifs in promoters of the rice genome and cellular-level implications on sheath blight disease resistance. Gene, 561, 209-218 https://doi.org/10.1016/j.gene.2015.02.031Singh Pooja, Siva Ramamoorthy, Gothandam Kodiveri M., Babu Subramanian (2013): Naturally Existing Levels of Osmyb 4 Gene Expression in Rice Cultivars Correlate with their Reaction to Fungal and Bacterial Pathogens. Journal of Phytopathology, 161, 730-734 https://doi.org/10.1111/jph.12114Soltész Alexandra, Vágújfalvi Attila, Rizza Fulvia, Kerepesi Ildikó, Galiba Gábor, Cattivelli Luigi, Coraggio Immacolata, Crosatti Cristina (2012): The rice Osmyb4 gene enhances tolerance to frost and improves germination under unfavourable conditions in transgenic barley plants. Journal of Applied Genetics, 53, 133-143 https://doi.org/10.1007/s13353-011-0081-xSriram S, Raguchander T, Babu S, Nandakumar R, Shanmugam V, Vidhyasekaran P, Balasubramanian P, Samiyappan. R (2000): Inactivation of phytotoxin produced by the rice sheath blight pathogen Rhizoctonia solani. Canadian Journal of Microbiology, 46, 520-524 https://doi.org/10.1139/w00-018Su’udi Mukhamad, Park Jong-Mi, Kang Woo-Ri, Hwang Duk-Ju, Kim Soonok, Ahn Il-Pyung (2013): Quantification of rice sheath blight progression caused by Rhizoctonia solani. Journal of Microbiology, 51, 380-388 https://doi.org/10.1007/s12275-013-3274-7Vannini Candida, Locatelli Franca, Bracale Marcella, Magnani Enrico, Marsoni Milena, Osnato Michela, Mattana Monica, Baldoni Elena, Coraggio Immacolata (2004): Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants. The Plant Journal, 37, 115-127 https://doi.org/10.1046/j.1365-313X.2003.01938.xVannini Candida, Iriti Marcello, Bracale Marcella, Locatelli Franca, Faoro Franco, Croce Paolo, Pirona Raul, Di Maro Antimo, Coraggio Immacolata, Genga Annamaria (2006): The ectopic expression of the rice Osmyb4 gene in Arabidopsis increases tolerance to abiotic, environmental and biotic stresses. Physiological and Molecular Plant Pathology, 69, 26-42 https://doi.org/10.1016/j.pmpp.2006.12.005Vannini Candida, Campa Manuela, Iriti Marcello, Genga Annamaria, Faoro Franco, Carravieri Sara, Rotino Giuseppe L., Rossoni Mara, Spinardi Anna, Bracale Marcella (2007): Evaluation of transgenic tomato plants ectopically expressing the rice Osmyb4 gene. Plant Science, 173, 231-239 https://doi.org/10.1016/j.plantsci.2007.05.007Vidhyasekaran P., Ponmalar T. Ruby, Samiyappan R., Velazhahan R., Vimala R., Ramanathan A., Paranidharan V., Muthukrishnan S. (1997): Host-Specific Toxin Production by Rhizoctonia solani , the Rice Sheath Blight Pathogen. Phytopathology, 87, 1258-1263 https://doi.org/10.1094/PHYTO.19220.127.116.118Zheng Aiping, Lin Runmao, Zhang Danhua, Qin Peigang, Xu Lizhi, Ai Peng, Ding Lei, Wang Yanran, Chen Yao, Liu Yao, Sun Zhigang, Feng Haitao, Liang Xiaoxing, Fu Rongtao, Tang Changqing, Li Qiao, Zhang Jing, Xie Zelin, Deng Qiming, Li Shuangcheng, Wang Shiquan, Zhu Jun, Wang Lingxia, Liu Huainian, Li Ping (2013): The evolution and pathogenic mechanisms of the rice sheath blight pathogen. Nature Communications, 4, 1424- https://doi.org/10.1038/ncomms2427