Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates
A. Eichmeier, M. Baránek, M. Pidrahttps://doi.org/10.17221/10/2010-PPSCitation:Eichmeier A., Baránek M., Pidra M. (2010): Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates. Plant Protect. Sci., 46: 145-148.
The genetic diversity of Grapevine fanleaf virus (GFLV) was evaluated in 4 isolates sampled from naturally infected grapevines from South Moravia (Czech Republic) and 2 Italian isolates from Bari (Italy). Conserved regions within sequences in databases were found and new primers corresponding to these regions were designed and tested for RT-PCR amplification of the CP codifying region. After sequencing of obtained amplicons the similarity of isolates was analysed via alignments of sequences and by means of dendrogramsKeywords:
Grapevine fanleaf virus; grapevine; coat protein; sequence